TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000081_2.0 # Protein # Selenoprotein O (SelO) # Human (Homo sapiens) (669 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472269310|gb|KB715347.1| Leptonychotes weddellii isolate WS11... 173 2e-50 gi|472267791|gb|KB716866.1| Leptonychotes weddellii isolate WS11... 73 6e-11 gi|472263686|gb|KB720971.1| Leptonychotes weddellii isolate WS11... 50 5e-04 gi|472269944|gb|KB714745.1| Leptonychotes weddellii isolate WS11... 50 5e-04 >gi|472269310|gb|KB715347.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00641, whole genome shotgun sequence Length = 1161464 Score = 173 bits (438), Expect(2) = 2e-50 Identities = 98/172 (56%), Positives = 104/172 (60%), Gaps = 49/172 (28%) Frame = -2 Query: 501 RQLSMMLMLAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQ 560 RQLSMMLMLAQSNPQLFAL+GTRA + +ELERVEQ SRLEQLS AEL S+N+GHWADWLQ Sbjct: 594721 RQLSMMLMLAQSNPQLFALIGTRANVTKELERVEQHSRLEQLSPAELLSKNRGHWADWLQ 594542 Query: 561 AY-------------------------------------------------RARLDKDLE 571 Y RARL+KD E Sbjct: 594541 EYG*GSRRPGADCSRHPLPGRWLQTTPPKQGTKCRRGGGVAPAGKMNLDDSRARLEKDRE 594362 Query: 572 GAGDAAAWQAEHVRVMHANNPKYVLRNYIAQNAIEAAERGDFSEVRRVLKLL 623 G D AWQAE RVMHANNPKYVLRNYIAQNAIEAAE GDFSE L LL Sbjct: 594361 GVSDKDAWQAERTRVMHANNPKYVLRNYIAQNAIEAAENGDFSEASTRLSLL 594206 Score = 146 bits (368), Expect = 5e-33 Identities = 70/101 (69%), Positives = 78/101 (77%) Frame = -2 Query: 351 PFGFLDRYDPDHVCNASDNTGRYAYSKQPEVCRWNLRKXXXXXXXXXXXXXXXXILAEEF 410 P RYDPDHVCNASDNTGRYAYSKQP+VC+WNL+K ILAEEF Sbjct: 598855 PLAPCGRYDPDHVCNASDNTGRYAYSKQPDVCKWNLQKLAEALEPELPRERGETILAEEF 598676 Query: 411 DAEFQRHYLQKMRRKLGLVQVELEEDGALVSKLLETMHLTG 451 D EF++HYLQKMRRKLGLV+ EL+EDGALV+KLLETMHLTG Sbjct: 598675 DPEFRQHYLQKMRRKLGLVRAELDEDGALVAKLLETMHLTG 598553 Score = 136 bits (342), Expect = 6e-30 Identities = 63/69 (91%), Positives = 69/69 (100%) Frame = -2 Query: 185 RQADGRKVLRSSIREFLCSEAMFHLGVPTTRAGACVTSESTVVRDVFYDGNPKYEQCTVV 244 RQADGRKVLRSSIREFLCSEAMFHLG+PTTRAGACVTS+STV+RDVFYDGNPKYE+CTVV Sbjct: 602020 RQADGRKVLRSSIREFLCSEAMFHLGIPTTRAGACVTSQSTVIRDVFYDGNPKYEKCTVV 601841 Query: 245 LRVASTFIR 253 LR+ASTF+R Sbjct: 601840 LRIASTFLR 601814 Score = 127 bits (318), Expect = 3e-27 Identities = 57/58 (98%), Positives = 57/58 (98%) Frame = -1 Query: 128 ALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTATGERWELQLKGAGPTPFSR 185 ALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTA GERWELQLKGAGPTPFSR Sbjct: 606746 ALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTAAGERWELQLKGAGPTPFSR 606573 Score = 118 bits (296), Expect = 1e-24 Identities = 57/62 (91%), Positives = 59/62 (95%) Frame = -2 Query: 253 RFGSFEIFKSADEHTGRAGPSVGRNDIRVQLLDYVISSFYPEIQAAHASDSVQRNAAFFR 312 RFGSFEIFKSADEHTGR GPSVGRNDIRVQ+LDYVIS+FYPEIQAAHA D VQRNAAFFR Sbjct: 600199 RFGSFEIFKSADEHTGREGPSVGRNDIRVQMLDYVISTFYPEIQAAHADDRVQRNAAFFR 600020 Query: 313 EV 314 EV Sbjct: 600019 EV 600014 Score = 97.1 bits (240), Expect = 4e-18 Identities = 43/45 (95%), Positives = 45/45 (100%) Frame = -3 Query: 313 EVTRRTARMVAEWQCVGFCHGVLNTDNMSILGLTIDYGPFGFLDR 357 +VTRRTARMVAEWQCVGFCHGVLNTDNMSI+GLTIDYGPFGFLDR Sbjct: 599391 QVTRRTARMVAEWQCVGFCHGVLNTDNMSIVGLTIDYGPFGFLDR 599257 Score = 86.7 bits (213), Expect = 5e-15 Identities = 44/57 (77%), Positives = 46/57 (80%) Frame = -2 Query: 445 ETMHLTGADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDPR 501 E + GADFTNTFYLLSSFPV ES GL EFLA L QCASLEEL+LAFRPQMDPR Sbjct: 595696 EQRRVPGADFTNTFYLLSSFPVGPESLGLPEFLAALTAQCASLEELKLAFRPQMDPR 595526 Score = 52.8 bits (125), Expect(2) = 2e-50 Identities = 31/58 (53%), Positives = 32/58 (55%), Gaps = 3/58 (5%) Frame = -3 Query: 615 EVRRVLKLLETPYHCXXXXXXXXXXXXXXXXXXRQ---RSYSSKPPLWAAELCVTXSS 669 +VRRVLKLLETPYH SYSSKPPLWAAELCVT SS Sbjct: 594129 QVRRVLKLLETPYHRDGEATEDLEATEPEGAGGADSGGHSYSSKPPLWAAELCVT*SS 593956 >gi|472267791|gb|KB716866.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02160, whole genome shotgun sequence Length = 521749 Score = 73.2 bits (178), Expect = 6e-11 Identities = 50/131 (38%), Positives = 58/131 (44%), Gaps = 59/131 (45%) Frame = +1 Query: 145 FGQFAGQLGDGAAMYLGEV-----------------------------CTATGERWELQL 175 FG +AGQLGDG A LG C GE+WELQL Sbjct: 386635 FGIWAGQLGDGRAHLLGIYMNRYHMFSGIIAFLLNINTDLLILRFLCCC*RQGEKWELQL 386814 Query: 176 KGAGPTPFSRQA------------------------------DGRKVLRSSIREFLCSEA 205 KG+G TP+SR + DGR VLRSS+REFLCSEA Sbjct: 386815 KGSGKTPYSRYSLIFFWSSK*KLILSSLQLKIFLLLFCPRNGDGRAVLRSSVREFLCSEA 386994 Query: 206 MFHLGVPTTRA 216 M LG+PT+RA Sbjct: 386995 MHSLGIPTSRA 387027 Score = 43.1 bits (100), Expect = 0.064 Identities = 21/35 (60%), Positives = 29/35 (82%) Frame = +3 Query: 589 ANNPKYVLRNYIAQNAIEAAERGDFSEVRRVLKLL 623 A NP+YVL+N++A++A+ AER DFSEV R L+LL Sbjct: 391584 AVNPRYVLKNWMAESAVRKAERNDFSEV-RCLRLL 391685 >gi|472263686|gb|KB720971.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold06266, whole genome shotgun sequence Length = 51140 Score = 50.1 bits (118), Expect = 5e-04 Identities = 20/36 (55%), Positives = 25/36 (69%) Frame = -1 Query: 325 WQCVGFCHGVLNTDNMSILGLTIDYGPFGFLDRYDP 360 W GV NTDN S+L +TIDYGPFGF++ Y+P Sbjct: 17510 WNLFVVVSGVCNTDNFSLLSITIDYGPFGFMEAYNP 17403 Score = 43.1 bits (100), Expect = 0.064 Identities = 21/35 (60%), Positives = 29/35 (82%) Frame = -2 Query: 589 ANNPKYVLRNYIAQNAIEAAERGDFSEVRRVLKLL 623 A NP+YVL+N++A++A+ AER DFSEV R L+LL Sbjct: 2782 AVNPRYVLKNWMAESAVRKAERNDFSEV-RCLRLL 2681 >gi|472269944|gb|KB714745.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00039, whole genome shotgun sequence Length = 4111496 Score = 50.1 bits (118), Expect = 5e-04 Identities = 29/50 (58%), Positives = 32/50 (64%) Frame = +1 Query: 451 GADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDP 500 GAD TNTFYLL S ES GL R+ Q ASLEELRL F+ Q+DP Sbjct: 184771 GADITNTFYLLISLSAVPESLGLTLSSQRI*LQGASLEELRLPFQSQVDP 184920 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.322 0.135 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 2,185,172,449 Number of Sequences: 16710 Number of extensions: 30139429 Number of successful extensions: 145757 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 347 Number of HSP's successfully gapped in prelim test: 277 Number of HSP's that attempted gapping in prelim test: 140376 Number of HSP's gapped (non-prelim): 17440 length of query: 669 length of database: 1,052,295,386 effective HSP length: 139 effective length of query: 530 effective length of database: 1,049,972,696 effective search space: 556485528880 effective search space used: 556485528880 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 81 (35.8 bits)