TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000079_2.0 # Protein # Selenoprotein N (SelN) # Walrus (Odobenus rosmarus) (504 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472267212|gb|KB717445.1| Leptonychotes weddellii isolate WS11... 153 2e-35 gi|472269546|gb|KB715143.1| Leptonychotes weddellii isolate WS11... 35 9.3 >gi|472267212|gb|KB717445.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02739, whole genome shotgun sequence Length = 322709 Score = 153 bits (387), Expect = 2e-35 Identities = 71/72 (98%), Positives = 72/72 (100%) Frame = -2 Query: 93 VSRLALSGLRNWTTAASPSAVFAARHFRPFLPPRGRVELGEPWWIIPSELSVFTGYLSNN 152 VSRLALSGLRNWTTAASPSAVFAARHFRPFLPP+GRVELGEPWWIIPSELSVFTGYLSNN Sbjct: 176977 VSRLALSGLRNWTTAASPSAVFAARHFRPFLPPQGRVELGEPWWIIPSELSVFTGYLSNN 176798 Query: 153 RFYPPPPKGKEV 164 RFYPPPPKGKEV Sbjct: 176797 RFYPPPPKGKEV 176762 Score = 131 bits (330), Expect = 1e-28 Identities = 63/67 (94%), Positives = 65/67 (97%) Frame = -2 Query: 276 IPQMELEALGPSVPSMILDEDGNMINSRPPSGEPLQFVFEEIKWQQELSWEEAARRLEVA 335 + QMELEALGPSVPSMILDEDGNMI+S PPSGEPLQFVFEEIKWQQELSWEEAARRLEVA Sbjct: 173728 LAQMELEALGPSVPSMILDEDGNMIDSHPPSGEPLQFVFEEIKWQQELSWEEAARRLEVA 173549 Query: 336 MYPFKKV 342 MYPFKKV Sbjct: 173548 MYPFKKV 173528 Score = 115 bits (289), Expect = 5e-24 Identities = 55/56 (98%), Positives = 56/56 (100%) Frame = -3 Query: 449 VHHINANHFLDITSMKPEDIENNVFSFSSTFEDPSTATYMQFLKEGLRRGLPLLQP 504 VHHINAN+FLDITSMKPEDIENNVFSFSSTFEDPSTATYMQFLKEGLRRGLPLLQP Sbjct: 170400 VHHINANYFLDITSMKPEDIENNVFSFSSTFEDPSTATYMQFLKEGLRRGLPLLQP 170233 Score = 105 bits (263), Expect = 6e-21 Identities = 47/47 (100%), Positives = 47/47 (100%) Frame = -3 Query: 205 RIHAEFQLSEPPDFPFWFSPGQFTGHIILSKDATHIRDFRLFVPNHR 251 RIHAEFQLSEPPDFPFWFSPGQFTGHIILSKDATHIRDFRLFVPNHR Sbjct: 175911 RIHAEFQLSEPPDFPFWFSPGQFTGHIILSKDATHIRDFRLFVPNHR 175771 Score = 97.1 bits (240), Expect = 3e-18 Identities = 50/64 (78%), Positives = 51/64 (79%) Frame = -2 Query: 2 EPLPPKQESALKALGTEGLFLFSSLDTDRDMYISPEEFKPIAEKLTGSAPAGGYEEAELA 61 EPLPPKQESALKALGTEGLFLFSSLD DRDMYISPEEFKPIAEKLTG+ G A Sbjct: 182809 EPLPPKQESALKALGTEGLFLFSSLDADRDMYISPEEFKPIAEKLTGTRRGWGLGRGARA 182630 Query: 62 LDPS 65 PS Sbjct: 182629 SWPS 182618 Score = 94.0 bits (232), Expect = 2e-17 Identities = 48/65 (73%), Positives = 50/65 (76%), Gaps = 4/65 (6%) Frame = -3 Query: 157 PPPKGKEVIIHRLLSMFHPRPFVKTRFAPQGAVACLTAISDFYYTVMFRI----HAEFQL 212 P P +VIIHRLLSMFHPRPFVKTRFAPQGAVACLTAISDFYYTVMFR H + L Sbjct: 176595 PLPAPSQVIIHRLLSMFHPRPFVKTRFAPQGAVACLTAISDFYYTVMFRCVVLRHPPWPL 176416 Query: 213 SEPPD 217 S D Sbjct: 176415 SRAGD 176401 Score = 91.3 bits (225), Expect = 1e-16 Identities = 46/54 (85%), Positives = 46/54 (85%) Frame = -3 Query: 41 PIAEKLTGSAPAGGYEEAELALDPSEETLTIEARFQPLRPESMTKSKDGFLGVS 94 P L GS PA YEEAELALDPSEETLTIEARFQPLRPESMTKSKDGFLGVS Sbjct: 179748 PATVCLLGSVPAASYEEAELALDPSEETLTIEARFQPLRPESMTKSKDGFLGVS 179587 Score = 81.3 bits (199), Expect = 1e-13 Identities = 38/46 (82%), Positives = 40/46 (86%) Frame = -1 Query: 338 PFKKVTYLPFIEAFDHAKAKNKLVHSILLWGALDDQSCXGSGRTLR 383 P+ +VTYLPFIEAFDHAKAKNKLVHSILLWGALDDQSC G G R Sbjct: 173183 PWPQVTYLPFIEAFDHAKAKNKLVHSILLWGALDDQSC*GEGDPAR 173046 Score = 77.4 bits (189), Expect(2) = 8e-29 Identities = 35/36 (97%), Positives = 36/36 (100%) Frame = -2 Query: 414 QNDQENPSHKKLADLHLEKYSFPVEMMICLPNGTVV 449 QN+QENPSHKKLADLHLEKYSFPVEMMICLPNGTVV Sbjct: 171793 QNNQENPSHKKLADLHLEKYSFPVEMMICLPNGTVV 171686 Score = 75.5 bits (184), Expect(2) = 8e-29 Identities = 38/38 (100%), Positives = 38/38 (100%) Frame = -3 Query: 377 GSGRTLRETVLESSPIIALLNESFISTWSLVRELEDLQ 414 GSGRTLRETVLESSPIIALLNESFISTWSLVRELEDLQ Sbjct: 171990 GSGRTLRETVLESSPIIALLNESFISTWSLVRELEDLQ 171877 Score = 64.7 bits (156), Expect = 1e-08 Identities = 30/42 (71%), Positives = 34/42 (80%) Frame = -2 Query: 247 VPNHRSLNVDMEWLYGAGESSNMEVDIGYIPQMELEALGPSV 288 +P RSLNVDMEWLYGAGESSNMEVDIGYIPQ+ + + V Sbjct: 174187 LPVTRSLNVDMEWLYGAGESSNMEVDIGYIPQVSTQGVPTQV 174062 >gi|472269546|gb|KB715143.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00437, whole genome shotgun sequence Length = 1311660 Score = 35.4 bits (80), Expect = 9.3 Identities = 23/73 (31%), Positives = 37/73 (50%), Gaps = 3/73 (4%) Frame = -1 Query: 286 PSVPSMIL---DEDGNMINSRPPSGEPLQFVFEEIKWQQELSWEEAARRLEVAMYPFKKV 342 P++PS+IL IN PPS F++ + + +S E+ R ++YPF Sbjct: 397167 PNLPSVILLRIQPCPPKINVEPPSTSKYDFIWNRV-FADVIS*EKVTRGKP*SLYPFPMH 396991 Query: 343 TYLPFIEAFDHAK 355 LPF+E+ D A+ Sbjct: 396990 HILPFLESLDTAE 396952 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.319 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 2,085,196,551 Number of Sequences: 16710 Number of extensions: 36366764 Number of successful extensions: 139670 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 644 Number of HSP's successfully gapped in prelim test: 692 Number of HSP's that attempted gapping in prelim test: 128569 Number of HSP's gapped (non-prelim): 33865 length of query: 504 length of database: 1,052,295,386 effective HSP length: 137 effective length of query: 367 effective length of database: 1,050,006,116 effective search space: 385352244572 effective search space used: 385352244572 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 80 (35.4 bits)