TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000079_2.0 # Protein # Selenoprotein N (SelN) # Human
(Homo sapiens)
(590 letters)
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno
me.fa
16,710 sequences; 3,156,886,159 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|472267212|gb|KB717445.1| Leptonychotes weddellii isolate WS11... 139 7e-31
gi|472265724|gb|KB718933.1| Leptonychotes weddellii isolate WS11... 36 6.7
>gi|472267212|gb|KB717445.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold02739, whole genome
shotgun sequence
Length = 322709
Score = 139 bits (349), Expect = 7e-31
Identities = 65/72 (90%), Positives = 68/72 (94%), Gaps = 1/72 (1%)
Frame = -2
Query: 180 VSRLALSGLRNWTAAASPSAVFATRHFQPFLPPPGQ-ELGEPWWIIPSELSMFTGYLSNN 238
VSRLALSGLRNWT AASPSAVFA RHF+PFLPP G+ ELGEPWWIIPSELS+FTGYLSNN
Sbjct: 176977 VSRLALSGLRNWTTAASPSAVFAARHFRPFLPPQGRVELGEPWWIIPSELSVFTGYLSNN 176798
Query: 239 RFYPPPPKGKEV 250
RFYPPPPKGKEV
Sbjct: 176797 RFYPPPPKGKEV 176762
Score = 127 bits (319), Expect = 2e-27
Identities = 61/67 (91%), Positives = 64/67 (95%)
Frame = -2
Query: 362 IPQMELEATGPSVPSVILDEDGSMIDSHLPSGEPLQFVFEEIKWQQELSWEEAARRLEVA 421
+ QMELEA GPSVPS+ILDEDG+MIDSH PSGEPLQFVFEEIKWQQELSWEEAARRLEVA
Sbjct: 173728 LAQMELEALGPSVPSMILDEDGNMIDSHPPSGEPLQFVFEEIKWQQELSWEEAARRLEVA 173549
Query: 422 MYPFKKV 428
MYPFKKV
Sbjct: 173548 MYPFKKV 173528
Score = 111 bits (278), Expect = 1e-22
Identities = 52/56 (92%), Positives = 56/56 (100%)
Frame = -3
Query: 535 VHHINANYFLDITSVKPEEIESNLFSFSSTFEDPSTATYMQFLKEGLRRGLPLLQP 590
VHHINANYFLDITS+KPE+IE+N+FSFSSTFEDPSTATYMQFLKEGLRRGLPLLQP
Sbjct: 170400 VHHINANYFLDITSMKPEDIENNVFSFSSTFEDPSTATYMQFLKEGLRRGLPLLQP 170233
Score = 103 bits (256), Expect = 4e-20
Identities = 45/47 (95%), Positives = 46/47 (97%)
Frame = -3
Query: 291 RIHAEFQLSEPPDFPFWFSPAQFTGHIILSKDATHVRDFRLFVPNHR 337
RIHAEFQLSEPPDFPFWFSP QFTGHIILSKDATH+RDFRLFVPNHR
Sbjct: 175911 RIHAEFQLSEPPDFPFWFSPGQFTGHIILSKDATHIRDFRLFVPNHR 175771
Score = 94.4 bits (233), Expect = 2e-17
Identities = 51/74 (68%), Positives = 53/74 (71%), Gaps = 5/74 (6%)
Frame = -3
Query: 243 PPPKGKEVIIHRLLSMFHPRPFVKTRFAPQGAVACLTAISDFYYTVMFRI----HAEFQL 298
P P +VIIHRLLSMFHPRPFVKTRFAPQGAVACLTAISDFYYTVMFR H + L
Sbjct: 176595 PLPAPSQVIIHRLLSMFHPRPFVKTRFAPQGAVACLTAISDFYYTVMFRCVVLRHPPWPL 176416
Query: 299 SEPPD-FPFWFSPA 311
S D W PA
Sbjct: 176415 SRAGDPREAWKGPA 176374
Score = 73.2 bits (178), Expect = 5e-11
Identities = 34/46 (73%), Positives = 38/46 (82%)
Frame = -1
Query: 424 PFKKVSYLPFTEAFDRAKAENKLVHSILLWGALDDQSCXGSGRTLR 469
P+ +V+YLPF EAFD AKA+NKLVHSILLWGALDDQSC G G R
Sbjct: 173183 PWPQVTYLPFIEAFDHAKAKNKLVHSILLWGALDDQSC*GEGDPAR 173046
Score = 68.2 bits (165), Expect = 2e-09
Identities = 32/37 (86%), Positives = 34/37 (91%)
Frame = -2
Query: 66 KTLGTDGLFLFSSLDTDGDMYISPEEFKPIAEKLTGS 102
K LGT+GLFLFSSLD D DMYISPEEFKPIAEKLTG+
Sbjct: 182776 KALGTEGLFLFSSLDADRDMYISPEEFKPIAEKLTGT 182666
Score = 62.0 bits (149), Expect = 1e-07
Identities = 29/42 (69%), Positives = 32/42 (76%)
Frame = -2
Query: 333 VPNHRSLNVDMEWLYGASESSNMEVDIGYIPQMELEATGPSV 374
+P RSLNVDMEWLYGA ESSNMEVDIGYIPQ+ + V
Sbjct: 174187 LPVTRSLNVDMEWLYGAGESSNMEVDIGYIPQVSTQGVPTQV 174062
Score = 60.8 bits (146), Expect = 3e-07
Identities = 29/32 (90%), Positives = 31/32 (96%)
Frame = -3
Query: 463 GSGRTLRETVLESSPILTLLNESFISTWSLVK 494
GSGRTLRETVLESSPI+ LLNESFISTWSLV+
Sbjct: 171990 GSGRTLRETVLESSPIIALLNESFISTWSLVR 171895
Score = 59.7 bits (143), Expect = 6e-07
Identities = 27/29 (93%), Positives = 28/29 (96%)
Frame = -2
Query: 507 SHQKLAGLHLEKYSFPVEMMICLPNGTVV 535
SH+KLA LHLEKYSFPVEMMICLPNGTVV
Sbjct: 171772 SHKKLADLHLEKYSFPVEMMICLPNGTVV 171686
Score = 55.1 bits (131), Expect = 1e-05
Identities = 39/83 (46%), Positives = 43/83 (51%), Gaps = 9/83 (10%)
Frame = -3
Query: 138 PAASCXXXXXXXXXXXXTLTIEARFQPLLPETMTKSKDGFLGVS------RLALSGLRNW 191
PAAS TLTIEARFQPL PE+MTKSKDGFLGVS RL G +
Sbjct: 179718 PAASYEEAELALDPSEETLTIEARFQPLRPESMTKSKDGFLGVSLSGGQWRLPPGGTTDT 179539
Query: 192 TAAASPSA---VFATRHFQPFLP 211
++A V A F FLP
Sbjct: 179538 GSSAHTDP*E*VCALFRFPSFLP 179470
>gi|472265724|gb|KB718933.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold04227, whole genome
shotgun sequence
Length = 169827
Score = 36.2 bits (82), Expect = 6.7
Identities = 23/66 (34%), Positives = 32/66 (48%), Gaps = 5/66 (7%)
Frame = +1
Query: 208 PFLPPPGQELGEPWWIIPSELSM-FTGYLS---NNRFYPPPPKGKEVIIHRLL-SMFHPR 262
P PPP ++G WW + S L F G S +++ PPPP G + H LL S+
Sbjct: 15328 PQCPPPQNQMGRVWWSLTSFLQFNFMGKTSLSCSHQQPPPPPIGVVITSHSLLFSLISAL 15507
Query: 263 PFVKTR 268
KT+
Sbjct: 15508 EMAKTK 15525
Score = 36.2 bits (82), Expect = 6.7
Identities = 23/66 (34%), Positives = 32/66 (48%), Gaps = 5/66 (7%)
Frame = +2
Query: 208 PFLPPPGQELGEPWWIIPSELSM-FTGYLS---NNRFYPPPPKGKEVIIHRLL-SMFHPR 262
P PPP ++G WW + S L F G S +++ PPPP G + H LL S+
Sbjct: 32480 PQCPPPQNQMGRVWWSLTSFLQFNFMGKTSLSCSHQQPPPPPIGVVITSHSLLFSLISAL 32659
Query: 263 PFVKTR 268
KT+
Sbjct: 32660 EMAKTK 32677
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge
nome.fa
Posted date: Oct 18, 2013 6:11 PM
Number of letters in database: 3,087,506,849
Number of sequences in database: 5788
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge
nome.fa.01
Posted date: Oct 18, 2013 6:11 PM
Number of letters in database: 69,379,310
Number of sequences in database: 10,922
Lambda K H
0.320 0.135 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,105,649,044
Number of Sequences: 16710
Number of extensions: 36440403
Number of successful extensions: 148481
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 700
Number of HSP's successfully gapped in prelim test: 734
Number of HSP's that attempted gapping in prelim test: 136543
Number of HSP's gapped (non-prelim): 36273
length of query: 590
length of database: 1,052,295,386
effective HSP length: 138
effective length of query: 452
effective length of database: 1,049,989,406
effective search space: 474595211512
effective search space used: 474595211512
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 81 (35.8 bits)