TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000077_2.0 # Protein # Selenoprotein M (SelM) # Human (Homo sapiens) (145 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472269525|gb|KB715164.1| Leptonychotes weddellii isolate WS11... 86 2e-26 >gi|472269525|gb|KB715164.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00458, whole genome shotgun sequence Length = 1321538 Score = 85.5 bits (210), Expect(2) = 2e-26 Identities = 42/54 (77%), Positives = 44/54 (81%) Frame = +3 Query: 92 LERIPLSEMTREEINALVQELGFYRKAAPDAQVPPEYVWAPAKPPEETSDHADL 145 L+RIPLSEMTREEIN LVQELGFYRKAAPD VPPEY+ APAKP E D DL Sbjct: 752409 LQRIPLSEMTREEINELVQELGFYRKAAPDEPVPPEYLRAPAKPAEGAPDRPDL 752570 Score = 55.5 bits (132), Expect(2) = 2e-26 Identities = 25/26 (96%), Positives = 25/26 (96%) Frame = +2 Query: 68 HNLVMKHLPGADPELVLLGRRYEELE 93 HNLVMKHLPGADPELVLLG RYEELE Sbjct: 752255 HNLVMKHLPGADPELVLLGHRYEELE 752332 Score = 35.0 bits (79), Expect = 0.89 Identities = 14/17 (82%), Positives = 15/17 (88%) Frame = +3 Query: 27 YRPDWNRLSGLTRARVE 43 +RPDWNRL GL RARVE Sbjct: 750228 FRPDWNRLHGLARARVE 750278 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.317 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 456,119,602 Number of Sequences: 16710 Number of extensions: 5775824 Number of successful extensions: 28268 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 574 Number of HSP's successfully gapped in prelim test: 235 Number of HSP's that attempted gapping in prelim test: 26336 Number of HSP's gapped (non-prelim): 5241 length of query: 145 length of database: 1,052,295,386 effective HSP length: 118 effective length of query: 27 effective length of database: 1,050,323,606 effective search space: 28358737362 effective search space used: 28358737362 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 70 (31.6 bits)