TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000074_2.0 # Protein # Selenoprotein I (SelI) # Human (Homo sapiens) (397 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472269591|gb|KB715098.1| Leptonychotes weddellii isolate WS11... 147 1e-33 gi|472269696|gb|KB714993.1| Leptonychotes weddellii isolate WS11... 64 1e-08 gi|472269593|gb|KB715096.1| Leptonychotes weddellii isolate WS11... 62 5e-08 gi|472269247|gb|KB715410.1| Leptonychotes weddellii isolate WS11... 37 2.3 gi|472269082|gb|KB715575.1| Leptonychotes weddellii isolate WS11... 35 8.7 >gi|472269591|gb|KB715098.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00392, whole genome shotgun sequence Length = 1373514 Score = 147 bits (371), Expect = 1e-33 Identities = 80/130 (61%), Positives = 86/130 (66%) Frame = +3 Query: 62 VFNFLLMAYFDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLG 121 +F F+ + + DF + G P W V FVA DGVDGKQARRTNSSTPLG Sbjct: 1149870 LFCFMFLQFIKEDFPHTGTGVDLFP---W*CV----FVA---DGVDGKQARRTNSSTPLG 1150019 Query: 122 ELFDHGLDSWSCVYFVVTVYSIFGRGSTGXXXXXXXXXXXXXXXXXILSHWEKYNTGILF 181 ELFDHGLDSWSCVYFVVTVYSIFGRG +G ILSHWEKYNTGILF Sbjct: 1150020 ELFDHGLDSWSCVYFVVTVYSIFGRGPSGVSVFVLYLLLWVVLFSFILSHWEKYNTGILF 1150199 Query: 182 LPWGYDISQV 191 LPWGYDISQV Sbjct: 1150200 LPWGYDISQV 1150229 Score = 122 bits (305), Expect = 5e-26 Identities = 59/72 (81%), Positives = 63/72 (87%) Frame = +3 Query: 242 FFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAFA 301 FFRSYKNNTLK NS+YEAMVP FSPCLLFILSTAWIL SPSDILELHPRVFYFMVGTAFA Sbjct: 1158162 FFRSYKNNTLKHNSIYEAMVPFFSPCLLFILSTAWILQSPSDILELHPRVFYFMVGTAFA 1158341 Query: 302 NSTCQLIVCQMS 313 N T + ++S Sbjct: 1158342 NITVRFFSFKIS 1158377 Score = 80.9 bits (198), Expect = 1e-13 Identities = 44/67 (65%), Positives = 48/67 (71%) Frame = +2 Query: 192 TISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTL 251 TISFVYIVTA+VGVEAWYEPFLFNFLYRDLFTAMIIG L V +Y N TL Sbjct: 1151360 TISFVYIVTAIVGVEAWYEPFLFNFLYRDLFTAMIIGKKLHV----------EAYFNITL 1151509 Query: 252 KLNSVYE 258 L V++ Sbjct: 1151510 LLKPVFD 1151530 Score = 79.0 bits (193), Expect = 5e-13 Identities = 36/36 (100%), Positives = 36/36 (100%) Frame = +3 Query: 43 VFPTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYAS 78 VFPTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYAS Sbjct: 1143594 VFPTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYAS 1143701 Score = 65.1 bits (157), Expect = 8e-09 Identities = 34/71 (47%), Positives = 36/71 (50%) Frame = +3 Query: 302 NSTCQLIVCQMSSTRCPTLNWXXXXXXXXXXXXXXXXASYVESIXXXXXXXXXXXXHIHY 361 N CQLIVCQMSSTRCPTLNW SYVESI HIHY Sbjct: 1159404 NFKCQLIVCQMSSTRCPTLNWFLVPLFLVVLVVNLGVPSYVESILLYTLTAVFTLAHIHY 1159583 Query: 362 GVRVVKQLSSH 372 GV+VV L + Sbjct: 1159584 GVQVVSTLEQN 1159616 Score = 62.8 bits (151), Expect = 4e-08 Identities = 31/36 (86%), Positives = 31/36 (86%) Frame = +2 Query: 362 GVRVVKQLSSHFQIYPFSLRKPNSDXLGMEEKNIGL 397 G VKQLSSHFQIYPFSLRKPNSD LGMEEKN GL Sbjct: 1161800 GFPQVKQLSSHFQIYPFSLRKPNSD*LGMEEKNSGL 1161907 Score = 59.3 bits (142), Expect = 4e-07 Identities = 28/43 (65%), Positives = 33/43 (76%) Frame = +2 Query: 75 FYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSS 117 F +APG+KHVPDWVWIVVGILNF+AYTL GK+ T+ S Sbjct: 1145852 FSFAAPGYKHVPDWVWIVVGILNFIAYTL----GKKMANTHVS 1145968 Score = 55.1 bits (131), Expect = 8e-06 Identities = 23/25 (92%), Positives = 25/25 (100%) Frame = +1 Query: 19 KYSAVDTNPLSLYVMHPFWNTIVKV 43 +YSAVDTNPLSLY+MHPFWNTIVKV Sbjct: 1143091 QYSAVDTNPLSLYIMHPFWNTIVKV 1143165 Score = 43.9 bits (102), Expect = 0.019 Identities = 19/19 (100%), Positives = 19/19 (100%) Frame = +2 Query: 1 MAGYEYVSPEQLAGFDKYK 19 MAGYEYVSPEQLAGFDKYK Sbjct: 1125869 MAGYEYVSPEQLAGFDKYK 1125925 Score = 41.6 bits (96), Expect = 0.093 Identities = 17/19 (89%), Positives = 19/19 (100%) Frame = +3 Query: 226 IIGCALCVTLPMSLLNFFR 244 ++GCALCVTLPMSLLNFFR Sbjct: 1156740 LLGCALCVTLPMSLLNFFR 1156796 >gi|472269696|gb|KB714993.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00287, whole genome shotgun sequence Length = 1616081 Score = 64.3 bits (155), Expect = 1e-08 Identities = 38/97 (39%), Positives = 50/97 (51%) Frame = +3 Query: 47 WLAPNLITFSGFLLVVFNFLLMAYFDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGV 106 WL N LL FN ++ YF P W +++ + F+ +LD + Sbjct: 823116 WLRLNCFQTEASLLS-FNSIIYIYF*------------APYWTYLLCALGLFIYQSLDAI 823256 Query: 107 DGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSI 143 DGKQARRTNS +PLGELFDHG DS S V +S+ Sbjct: 823257 DGKQARRTNSCSPLGELFDHGCDSLSTGKLVKVFFSL 823367 >gi|472269593|gb|KB715096.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00390, whole genome shotgun sequence Length = 1441730 Score = 62.4 bits (150), Expect = 5e-08 Identities = 29/47 (61%), Positives = 34/47 (72%) Frame = +1 Query: 86 PDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWS 132 P W +I F+ +LD +DGKQARRTNSS+PLGELFDHG DS S Sbjct: 1140025 PLWAYIACACGLFIYQSLDAIDGKQARRTNSSSPLGELFDHGCDSLS 1140165 Score = 40.4 bits (93), Expect = 0.21 Identities = 19/67 (28%), Positives = 37/67 (55%) Frame = +2 Query: 7 VSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLLVVFNFL 66 +S QL ++++Y + + L +M +W +V P+W+APNLIT G + + + Sbjct: 1134446 LSRHQLKRLEEHRYQSAGRSLLEP-LMQGYWEWLVGRVPSWIAPNLITIIGLSINICTTV 1134622 Query: 67 LMAYFDP 73 L+ ++ P Sbjct: 1134623 LLVFYCP 1134643 >gi|472269247|gb|KB715410.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00704, whole genome shotgun sequence Length = 1000651 Score = 37.0 bits (84), Expect = 2.3 Identities = 23/68 (33%), Positives = 30/68 (44%), Gaps = 7/68 (10%) Frame = +3 Query: 212 FLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSP----- 266 ++F FLYR L C C +S +FFR Y N L + E +PLF P Sbjct: 426147 YIFKFLYRTLCL-----CPCCFVCFLSCGSFFRDYYQNNAGLIKLIEIHIPLFCPPEEFV 426311 Query: 267 --CLLFIL 272 C L +L Sbjct: 426312 *NCALLVL 426335 >gi|472269082|gb|KB715575.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00869, whole genome shotgun sequence Length = 931946 Score = 35.0 bits (79), Expect = 8.7 Identities = 20/61 (32%), Positives = 35/61 (57%) Frame = -2 Query: 256 VYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAFANSTCQLIVCQMSST 315 +Y+ ++PLF PCL F S++ + WS ++LHP F +V + S+C CQ + + Sbjct: 362137 IYK*ILPLFYPCL*FESSSS-VTWS----MQLHPTFFLLLVSPT-STSSCYCSDCQNNHS 361976 Query: 316 R 316 + Sbjct: 361975 K 361973 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.327 0.141 0.463 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,616,695,279 Number of Sequences: 16710 Number of extensions: 25971643 Number of successful extensions: 183484 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 888 Number of HSP's successfully gapped in prelim test: 1093 Number of HSP's that attempted gapping in prelim test: 156277 Number of HSP's gapped (non-prelim): 67148 length of query: 397 length of database: 1,052,295,386 effective HSP length: 134 effective length of query: 263 effective length of database: 1,050,056,246 effective search space: 276164792698 effective search space used: 276164792698 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 79 (35.0 bits)