Command line: [exonerate -m p2g --showtargetgff -q ./SecS.fa -t SecS.carpeta/genomic.fa] Hostname: [ws101071] C4 Alignment: ------------ Query: SPP00000065_2.0 # Protein # Selenocysteine synthase (SecS) # Human (Homo sapiens) Target: gi|472268114|gb|KB716543.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01837, whole genome shotgun sequence Model: protein2genome:local Raw score: 2317 Query range: 0 -> 500 Target range: 339255 -> 376509 1 : MetAsnArgGluSerPheAlaAlaGlyGluArgLeuValSerProAlaTyrValArgG : 20 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MetAsnArgGluSerPheAlaAlaGlyGluArgLeuValSerProAlaTyrValArgG 339256 : ATGAACCGCGAGAGCTTCGCGGCGGGCGAGCGGCTGGTGTCGCCGGCTTATGTGCGAC : 339313 21 : lnGlyCysGluAlaArgArgSerHisGluHisLeuIleArgLeuLeuLeuGluLys : 39 |||||||||||||||||||||||||||||||||||||||||||||||||||||||| lnGlyCysGluAlaArgArgSerHisGluHisLeuIleArgLeuLeuLeuGluLys++ 339314 : AGGGCTGTGAGGCCCGCCGCTCGCATGAGCACCTCATACGGCTGCTTCTGGAGAAGgt : 339370 40 : >>>> Target Intron 1 >>>> GlyLysCysProGluAsnGlyTrpAspGluS : 49 808 bp |||||||||||||||:!!||||||||||||| ++GlyLysCysProGluAspGlyTrpAspGluS 339371 : .........................agGGCAAGTGTCCAGAGGATGGTTGGGATGAAA : 340208 50 : erThrLeuGluLeuPheLeuHisGluLeuAlaIleMetAspSerAsnAsnPheLeuGl : 68 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| erThrLeuGluLeuPheLeuHisGluLeuAlaIleMetAspSerAsnAsnPheLeuGl 340209 : GTACACTTGAACTTTTTTTACATGAACTTGCAATCATGGATAGCAACAATTTCCTGGG : 340265 69 : yAsnCysGlyValGlyGluArgGluGlyArgValAlaSerAlaLeuValAlaArgArg : 87 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| yAsnCysGlyValGlyGluArgGluGlyArgValAlaSerAlaLeuValAlaArgArg 340266 : CAATTGTGGTGTGGGAGAAAGGGAAGGGAGAGTGGCATCTGCCTTAGTTGCTCGGCGT : 340322 88 : HisTyr{Ar} >>>> Target Intron 2 >>>> {g}PheIleHisGlyIleG : 96 ||||||{||} 2573 bp {|}|||||||||||||||| HisTyr{Ar}++ ++{g}PheIleHisGlyIleG 340323 : CATTAC{AG}gt.........................ag{G}TTCATTCATGGAATTG : 342922 97 : lyArgSerGlyAspIleSerAlaValGlnProLysAlaAlaGlySerSerLeuLeuAs : 115 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lyArgSerGlyAspIleSerAlaValGlnProLysAlaAlaGlySerSerLeuLeuAs 342923 : GACGATCGGGTGATATTTCTGCTGTGCAGCCAAAAGCTGCAGGTTCTAGCCTTTTGAA : 342979 116 : nLysIleThrAsnSerLeuValLeuAspIleIleLysLeuAla{G} >>>> Targe : 130 |!:!|||||||||||||||||||||||||||||||||||||||{|} nArgIleThrAsnSerLeuValLeuAspIleIleLysLeuAla{G}++ 342980 : CAGAATTACCAATTCTTTGGTCCTGGACATCATAAAGTTGGCT{G}gt.......... : 343027 131 : t Intron 3 >>>> {ly}ValHisThrValAlaAsnCysPheValValProMetA : 143 319 bp {||}|||! !||||||.!!|||||||||||||||||||||| ++{ly}ValProThrValThrAsnCysPheValValProMetA 343028 : ...............ag{GT}GTCCCTACAGTGACCAACTGCTTTGTAGTTCCCATGG : 343382 144 : laThrGlyMetSerLeuThrLeuCysPheLeuThrLeuArgHisLysArgProLysAl : 162 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| laThrGlyMetSerLeuThrLeuCysPheLeuThrLeuArgHisLysArgProLysAl 343383 : CAACTGGTATGAGTCTAACCTTGTGTTTCTTAACATTACGACACAAAAGACCAAAGGC : 343439 163 : aLysTyrIleIleTrpProArgIleAspGlnLysSerCysPheLysSerMetIleThr : 181 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| aLysTyrIleIleTrpProArgIleAspGlnLysSerCysPheLysSerMetIleThr 343440 : AAAGTATATTATATGGCCGAGAATAGACCAGAAGTCCTGCTTTAAATCCATGATCACT : 343496 182 : Ala{G} >>>> Target Intron 4 >>>> {ly}PheGluProValValIleG : 190 |||{|} 3077 bp {||}||||||||||||||||||| Ala{G}++ ++{ly}PheGluProValValIleG 343497 : GCA{G}gt.........................ag{GT}TTTGAGCCTGTGGTGATAG : 346600 191 : luAsnValLeuGluGlyAspGluLeuArgThrAspLeuLysAlaValGluAlaLysVa : 209 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| luAsnValLeuGluGlyAspGluLeuArgThrAspLeuLysAlaValGluAlaLysVa 346601 : AGAATGTTTTAGAAGGTGACGAGCTGCGCACAGACCTGAAAGCAGTGGAGGCTAAAGT : 346657 210 : lGlnGluLeuGlyProAspCysIleLeuCysIleHisSerThrThrSerCysPheAla : 228 |!:!|||||||||||||||! !|||||||||:!!|||||||||||||||||||||||| lArgGluLeuGlyProAspSerIleLeuCysValHisSerThrThrSerCysPheAla 346658 : TCGGGAACTTGGGCCTGATTCCATTCTGTGTGTTCATTCCACTACATCCTGTTTTGCT : 346714 229 : ProArgValProAsp{Ar} >>>> Target Intron 5 >>>> {g}LeuGluG : 237 |||||||||||||||{||} 2879 bp {|}||||||| ProArgValProAsp{Ar}++ ++{g}LeuGluG 346715 : CCAAGGGTGCCTGAT{AG}gt.........................ag{A}TTAGAAG : 349620 238 : luLeuAlaValIleCysAlaAsnTyrAspIleProHisIleValAsnAsnAlaTyrGl : 256 ||||||||||||||||||||||||||! !||||||||||||||||||||||||||||| luLeuAlaValIleCysAlaAsnTyrGlyIleProHisIleValAsnAsnAlaTyrGl 349621 : AACTGGCTGTGATTTGTGCTAATTACGGCATTCCCCATATAGTCAACAATGCTTATGG : 349677 257 : yValGlnSerSerLysCysMetHisLeuIleGlnGln >>>> Target Intron : 269 ||||||||||||||||||||||||||||||||||||| 12501 bp yValGlnSerSerLysCysMetHisLeuIleGlnGln++ 349678 : AGTGCAGTCTTCAAAGTGTATGCACCTCATCCAGCAGgt................... : 349718 270 : 6 >>>> GlyAlaArgValGlyArgIleAspAlaPheValGlnSerLeuAspLysAs : 285 |||||||||||||||||||||||||||||||||||||||||||||||||| ++GlyAlaArgValGlyArgIleAspAlaPheValGlnSerLeuAspLysAs 349719 : ......agGGGGCTCGGGTTGGCAGAATAGATGCTTTTGTTCAGAGCTTGGACAAAAA : 362265 286 : nPheMetValProValGlyGlyAlaIleIleAlaGlyPheAsnAspSerPheIleGln : 304 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| nPheMetValProValGlyGlyAlaIleIleAlaGlyPheAsnAspSerPheIleGln 362266 : TTTTATGGTTCCGGTAGGTGGCGCTATAATTGCTGGCTTTAATGATTCCTTCATTCAG : 362322 305 : GluIleSerLysMetTyrPro{G} >>>> Target Intron 7 >>>> {ly}A : 313 |||||||||||||||||||||{|} 133 bp {||}| GluIleSerLysMetTyrPro{G}++ ++{ly}A 362323 : GAAATCAGCAAGATGTATCCA{G}gt.........................ag{GA}A : 362482 314 : rgAlaSerAlaSerProSerLeuAspValLeuIleThrLeuLeuSerLeuGlySerAs : 332 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| rgAlaSerAlaSerProSerLeuAspValLeuIleThrLeuLeuSerLeuGlySerAs 362483 : GAGCTTCAGCTTCACCTTCTTTAGATGTCCTTATTACTTTATTATCACTTGGATCAAA : 362539 333 : nGlyTyrLysLysLeuLeuLysGluArgLys >>>> Target Intron 8 >>>> : 343 ||||||||||||||||||||||||||||||| 9844 bp nGlyTyrLysLysLeuLeuLysGluArgLys++ 362540 : TGGCTATAAGAAGCTATTAAAAGAAAGAAAGgt......................... : 362574 344 : GluMetPheSerTyrLeuSerAsnGlnIleLysLysLeuSerGluAlaTyrAsnGl : 361 |||||||||||||||||||||!:!|||:!!||||||||||||||| !|||||||| ++GluMetPheSerTyrLeuSerSerGlnLeuLysLysLeuSerGluAsnTyrAsnGl 362575 : agGAGATGTTTTCATACTTGTCCAGCCAACTAAAGAAGCTGTCAGAAAACTACAATGA : 372470 362 : uArgLeuLeuHisThrProHisAsnProIleSerLeu{A} >>>> Target Intr : 374 |||||||||||||||||||||||||||||||||||||{|} 2199 bp uArgLeuLeuHisThrProHisAsnProIleSerLeu{A}++ 372471 : AAGGCTGTTGCATACACCTCACAATCCTATATCTTTA{G}gt................ : 372512 375 : on 9 >>>> {la}MetThrLeuLysThrLeuAspGluHisArgAspLysAlaValT : 389 {||}:!!|||||||||||||||! !||||||!:!|||!:!|||:!!| ++{la}ValThrLeuLysThrLeuGlyGluHisGlnAspArgAlaIleT 372513 : .........ag{CT}GTGACACTTAAGACACTAGGTGAACACCAAGACAGAGCCATCA : 374753 390 : hrGlnLeuGlySerMetLeuPheThrArgGlnValSerGlyAla{Ar} >>>> Tar : 404 ||||||||||||||||||||||||||||||||||||||||||||{||} hrGlnLeuGlySerMetLeuPheThrArgGlnValSerGlyAla{Ar}++ 374754 : CTCAGCTTGGCTCAATGCTTTTTACCAGACAGGTTTCTGGAGCC{AG}gt........ : 374802 405 : get Intron 10 >>>> {g}ValValProLeuGlySerMetGlnThrValSerGl : 416 1409 bp {|}||||||||||||||||||:!!|||||||||||||| ++{g}ValValProLeuGlySerValGlnThrValSerGl 374803 : ..................ag{G}GTTGTGCCTCTTGGGTCTGTGCAAACCGTGAGTGG : 376243 417 : yTyrThrPheArgGlyPheMetSerHisThrAsnAsnTyrProCysAlaTyrLeuAsn : 435 |:!!||||||||||||||||||||||||.!!|||:!!||||||||||||||||||||| yHisThrPheArgGlyPheMetSerHisAlaAsnHisTyrProCysAlaTyrLeuAsn 376244 : CCACACTTTCCGAGGCTTTATGTCACATGCAAATCATTACCCTTGCGCTTACCTCAAT : 376300 436 : AlaAlaSerAlaIleGlyMetLysMetGlnAspValAspLeuPheIleLysArgLeuA : 455 ||||||:!!|||||||||||||||! ||||||||||||||||||!!:|||||||||| AlaAlaAlaAlaIleGlyMetLysThrGlnAspValAspLeuPheMetLysArgLeuA 376301 : GCTGCAGCGGCCATCGGAATGAAGACACAGGATGTGGACTTGTTCATGAAGAGACTTG : 376360 456 : spArgCysLeuLysAlaValArgLysGluArgSerLysGluSerAsp<-><-><-><- : 471 ||!:!|||||||||.!!||||||||||||!:!!.!|||||||||:!! spLysCysLeuLysThrValArgLysGluGlnLysLysGluSerAsnAlaSerGlyVa 376361 : ACAAGTGTTTAAAGACAGTAAGGAAAGAACAAAAGAAAGAGAGTAATGCATCTGGAGT : 376417 472 : >AspAsnTyrAspLysThrGluAspValAspIleGluGluMetAlaLeuLysLeuAsp : 489 ||||||||||||! !|||:!!|||||||||||||||||||||||||||||||||||| lAspAsnTyrAspThrThrLysAspValAspIleGluGluMetAlaLeuLysLeuAsp 376418 : TGACAATTACGACACAACTAAAGATGTGGATATTGAAGAAATGGCTTTAAAACTAGAT : 376474 490 : AsnValLeuLeuAspThrTyrGlnAspAlaSer : 500 ||||||||||||||||||||||||:!!:!!||| AsnValLeuLeuAspThrTyrGlnAsnSerSer 376475 : AATGTGCTTCTTGACACGTACCAGAATTCTTCA : 376509 vulgar: SPP00000065_2.0 0 500 . gi|472268114|gb|KB716543.1| 339255 376509 + 2317 M 38 114 5 0 2 I 0 804 3 0 2 M 51 153 S 0 2 5 0 2 I 0 2569 3 0 2 S 1 1 M 39 117 S 0 1 5 0 2 I 0 315 3 0 2 S 1 2 M 52 156 S 0 1 5 0 2 I 0 3073 3 0 2 S 1 2 M 50 150 S 0 2 5 0 2 I 0 2875 3 0 2 S 1 1 M 34 102 5 0 2 I 0 12497 3 0 2 M 43 129 S 0 1 5 0 2 I 0 129 3 0 2 S 1 2 M 30 90 5 0 2 I 0 9840 3 0 2 M 31 93 S 0 1 5 0 2 I 0 2195 3 0 2 S 1 2 M 29 87 S 0 2 5 0 2 I 0 1405 3 0 2 S 1 1 M 66 198 G 0 12 M 30 90 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472268114|gb|KB716543.1| exonerate:protein2genome:local gene 339256 376509 2317 + . gene_id 1 ; sequence SPP00000065_2.0 ; gene_orientation + gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 339256 339369 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 339256 339369 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 339370 339371 . + . intron_id 1 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 339370 340177 . + . intron_id 1 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 340176 340177 . + . intron_id 0 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 340178 340332 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 340178 340332 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 340333 340334 . + . intron_id 2 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 340333 342905 . + . intron_id 2 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 342904 342905 . + . intron_id 1 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 342906 343024 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 342906 343024 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 343025 343026 . + . intron_id 3 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 343025 343343 . + . intron_id 3 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 343342 343343 . + . intron_id 2 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 343344 343502 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 343344 343502 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 343503 343504 . + . intron_id 4 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 343503 346579 . + . intron_id 4 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 346578 346579 . + . intron_id 3 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 346580 346733 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 346580 346733 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 346734 346735 . + . intron_id 5 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 346734 349612 . + . intron_id 5 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 349611 349612 . + . intron_id 4 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 349613 349715 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 349613 349715 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 349716 349717 . + . intron_id 6 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 349716 362216 . + . intron_id 6 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 362215 362216 . + . intron_id 5 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 362217 362346 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 362217 362346 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 362347 362348 . + . intron_id 7 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 362347 362479 . + . intron_id 7 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 362478 362479 . + . intron_id 6 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 362480 362571 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 362480 362571 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 362572 362573 . + . intron_id 8 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 362572 372415 . + . intron_id 8 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 372414 372415 . + . intron_id 7 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 372416 372509 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 372416 372509 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 372510 372511 . + . intron_id 9 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 372510 374708 . + . intron_id 9 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 374707 374708 . + . intron_id 8 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 374709 374799 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 374709 374799 . + . insertions 0 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice5 374800 374801 . + . intron_id 10 ; splice_site "GT" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local intron 374800 376208 . + . intron_id 10 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local splice3 376207 376208 . + . intron_id 9 ; splice_site "AG" gi|472268114|gb|KB716543.1| exonerate:protein2genome:local cds 376209 376509 . + . gi|472268114|gb|KB716543.1| exonerate:protein2genome:local exon 376209 376509 . + . insertions 12 ; deletions 0 gi|472268114|gb|KB716543.1| exonerate:protein2genome:local similarity 339256 376509 2317 + . alignment_id 1 ; Query SPP00000065_2.0 ; Align 339256 1 114 ; Align 340178 39 153 ; Align 342907 91 117 ; Align 343346 131 156 ; Align 346582 184 150 ; Align 349614 235 102 ; Align 362217 269 129 ; Align 362482 313 90 ; Align 372416 343 93 ; Align 374711 375 87 ; Align 376210 405 198 ; Align 376420 471 90 # --- END OF GFF DUMP --- # -- completed exonerate analysis