TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000065_2.0 # Protein # Selenocysteine synthase (SecS) #
Human (Homo sapiens)
(501 letters)
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno
me.fa
16,710 sequences; 3,156,886,159 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|472268114|gb|KB716543.1| Leptonychotes weddellii isolate WS11... 164 1e-38
gi|472269949|gb|KB714740.1| Leptonychotes weddellii isolate WS11... 35 9.2
>gi|472268114|gb|KB716543.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold01837, whole genome
shotgun sequence
Length = 523432
Score = 164 bits (416), Expect = 1e-38
Identities = 81/101 (80%), Positives = 92/101 (91%), Gaps = 4/101 (3%)
Frame = +1
Query: 404 RVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFIKRLDRCLKAVRK 463
RVVPLGS+QTVSG+TFRGFMSH N+YPCAYLNAA+AIGMK QDVDLF+KRLD+CLK VRK
Sbjct: 376207 RVVPLGSVQTVSGHTFRGFMSHANHYPCAYLNAAAAIGMKTQDVDLFMKRLDKCLKTVRK 376386
Query: 464 ERSKESD----DNYDKTEDVDIEEMALKLDNVLLDTYQDAS 500
E+ KES+ DNYD T+DVDIEEMALKLDNVLLDTYQ++S
Sbjct: 376387 EQKKESNASGVDNYDTTKDVDIEEMALKLDNVLLDTYQNSS 376509
Score = 114 bits (285), Expect = 2e-23
Identities = 53/59 (89%), Positives = 56/59 (94%)
Frame = +2
Query: 34 LLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCGVGEREGRVASALVARRHYRFI 92
L +GKCPE+GWDESTLELFLHELAIMDSNNFLGNCGVGEREGRVASALVARRHYR+I
Sbjct: 340163 LFFFQGKCPEDGWDESTLELFLHELAIMDSNNFLGNCGVGEREGRVASALVARRHYRYI 340339
Score = 111 bits (277), Expect = 1e-22
Identities = 52/54 (96%), Positives = 52/54 (96%)
Frame = +2
Query: 130 GVHTVANCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAG 183
GV TV NCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAG
Sbjct: 343343 GVPTVTNCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAG 343504
Score = 104 bits (259), Expect = 2e-20
Identities = 49/52 (94%), Positives = 51/52 (98%)
Frame = +1
Query: 183 GFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDR 234
GFEPVVIENVLEGDELRTDLKAVEAKV+ELGPD ILC+HSTTSCFAPRVPDR
Sbjct: 346579 GFEPVVIENVLEGDELRTDLKAVEAKVRELGPDSILCVHSTTSCFAPRVPDR 346734
Score = 91.7 bits (226), Expect = 1e-16
Identities = 44/45 (97%), Positives = 45/45 (100%)
Frame = +3
Query: 269 GARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGR 313
GARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPG+
Sbjct: 362217 GARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGK 362351
Score = 82.0 bits (201), Expect = 9e-14
Identities = 42/52 (80%), Positives = 47/52 (90%)
Frame = +1
Query: 83 LVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAGVHTV 134
L+A RFIHGIGRSGDISAVQPKAAGSSLLN+ITNSLVLDIIKLAG++ +
Sbjct: 342883 LLAHFLLRFIHGIGRSGDISAVQPKAAGSSLLNRITNSLVLDIIKLAGMYYI 343038
Score = 79.0 bits (193), Expect = 7e-13
Identities = 38/38 (100%), Positives = 38/38 (100%)
Frame = +1
Query: 1 MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEK 38
MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEK
Sbjct: 339256 MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEK 339369
Score = 73.2 bits (178), Expect = 4e-11
Identities = 34/35 (97%), Positives = 34/35 (97%)
Frame = +3
Query: 234 RLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQ 268
RLEELAVICANY IPHIVNNAYGVQSSKCMHLIQQ
Sbjct: 349611 RLEELAVICANYGIPHIVNNAYGVQSSKCMHLIQQ 349715
Score = 63.5 bits (153), Expect = 3e-08
Identities = 32/36 (88%), Positives = 33/36 (91%)
Frame = +1
Query: 312 GRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSY 347
GRASASPSLDVLITLLSLGSNGYKKLLKERK F +
Sbjct: 362479 GRASASPSLDVLITLLSLGSNGYKKLLKERKVSFPF 362586
Score = 62.0 bits (149), Expect = 9e-08
Identities = 28/32 (87%), Positives = 31/32 (96%)
Frame = +2
Query: 342 KEMFSYLSNQIKKLSEAYNERLLHTPHNPISL 373
+EMFSYLS+Q+KKLSE YNERLLHTPHNPISL
Sbjct: 372413 QEMFSYLSSQLKKLSENYNERLLHTPHNPISL 372508
Score = 55.8 bits (133), Expect = 7e-06
Identities = 26/31 (83%), Positives = 30/31 (96%)
Frame = +2
Query: 374 AMTLKTLDEHRDKAVTQLGSMLFTRQVSGAR 404
A+TLKTL EH+D+A+TQLGSMLFTRQVSGAR
Sbjct: 374708 AVTLKTLGEHQDRAITQLGSMLFTRQVSGAR 374800
>gi|472269949|gb|KB714740.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00034, whole genome
shotgun sequence
Length = 3714458
Score = 35.4 bits (80), Expect = 9.2
Identities = 21/76 (27%), Positives = 38/76 (50%)
Frame = -1
Query: 12 LVSPAYVRQGCEARRSHEHLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCG 71
++ + V+ GC+ R+ ++ LI + + P GWD+ + + E I GN G
Sbjct: 619382 IIFSSCVQHGCQRRQINDGLIL----RWELPTRGWDKDPSSITVMEQGIQVDQEAHGNRG 619215
Query: 72 VGEREGRVASALVARR 87
VG + R + +V+RR
Sbjct: 619214 VGGQCVRASGFVVSRR 619167
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge
nome.fa
Posted date: Oct 18, 2013 6:11 PM
Number of letters in database: 3,087,506,849
Number of sequences in database: 5788
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge
nome.fa.01
Posted date: Oct 18, 2013 6:11 PM
Number of letters in database: 69,379,310
Number of sequences in database: 10,922
Lambda K H
0.320 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,754,471,613
Number of Sequences: 16710
Number of extensions: 25802222
Number of successful extensions: 109793
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 470
Number of HSP's successfully gapped in prelim test: 465
Number of HSP's that attempted gapping in prelim test: 102788
Number of HSP's gapped (non-prelim): 21460
length of query: 501
length of database: 1,052,295,386
effective HSP length: 137
effective length of query: 364
effective length of database: 1,050,006,116
effective search space: 382202226224
effective search space used: 382202226224
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 80 (35.4 bits)