TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000064_2.0 # Protein # Selenophosphate synthetase 2 (SPS2) # Human (Homo sapiens) (483 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472269029|gb|KB715628.1| Leptonychotes weddellii isolate WS11... 744 0.0 gi|472269736|gb|KB714953.1| Leptonychotes weddellii isolate WS11... 167 4e-68 gi|472269855|gb|KB714834.1| Leptonychotes weddellii isolate WS11... 124 2e-26 gi|472269647|gb|KB715042.1| Leptonychotes weddellii isolate WS11... 37 3.0 gi|472269897|gb|KB714792.1| Leptonychotes weddellii isolate WS11... 37 3.0 gi|472268182|gb|KB716475.1| Leptonychotes weddellii isolate WS11... 37 3.9 gi|472268794|gb|KB715863.1| Leptonychotes weddellii isolate WS11... 36 5.1 gi|472266711|gb|KB717946.1| Leptonychotes weddellii isolate WS11... 36 6.7 >gi|472269029|gb|KB715628.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00922, whole genome shotgun sequence Length = 925757 Score = 744 bits (1921), Expect = 0.0 Identities = 383/461 (83%), Positives = 392/461 (85%), Gaps = 3/461 (0%) Frame = +1 Query: 26 LTLGRSFSNYRPFEPQALGLSPSWRLTGFSGMKGUGCKVPQEALLKLLAGLTRPDVRPPL 85 L+LGR FS+YRPFEPQALGLSPSWRLTGFSGMKG GCKVPQE LLKLLAGLTRPDVRPPL Sbjct: 469714 LSLGRGFSSYRPFEPQALGLSPSWRLTGFSGMKG*GCKVPQETLLKLLAGLTRPDVRPPL 469893 Query: 86 GRGL--VGGQEEASQEAGLPAGAGPSPTFPALGIGMDSCVIPLRHGGLSLVQTTDFFYPL 143 G GL VGG EEA+QE GLPAGA P+ TFPALGIGMDSCVIPLRHGGLSLVQTTDFFYPL Sbjct: 469894 GLGLGLVGGHEEAAQEGGLPAGAEPNATFPALGIGMDSCVIPLRHGGLSLVQTTDFFYPL 470073 Query: 144 VEDPYMMGRIACANVLSDLYAMGITECDNXXXXXXXXXXXXXXXREKVTPLMVKGFRDXX 203 VEDPYMMGRIACANVLSDLYAMGITECDN REK+TPLMVKGFRD Sbjct: 470074 VEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMTEEEREKITPLMVKGFRDAA 470253 Query: 204 XXXXXXXXXXXXXXNPWIIIGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAH 263 NPWII+GGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAH Sbjct: 470254 EEGGTAVTGGQTVVNPWIIVGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAH 470433 Query: 264 QWLDNPERWNKVKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGIL 323 QWLDNPERWNK+ MVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGIL Sbjct: 470434 QWLDNPERWNKINMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGIL 470613 Query: 324 GHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASGRFGLLQGTSAETSGGLLICLPREQA 383 GHSQNLAKQQRNEVSFVIHNLPIIAKMAA+SKASGRFGLLQGTSAETSGGLLICLPREQA Sbjct: 470614 GHSQNLAKQQRNEVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETSGGLLICLPREQA 470793 Query: 384 ARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATAAVLXXXXXXXX 443 ARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATAA L Sbjct: 470794 ARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATAAALAPDNSSAS 470973 Query: 444 XXXXXXXXRTEVVWTLEPLSTITDGSQELIV-RNFQRRLPA 483 EVVWTLEPL TI SQ I+ RNFQRRL A Sbjct: 470974 SEPSS*DGIAEVVWTLEPLYTIISDSQNFILRRNFQRRLSA 471096 >gi|472269736|gb|KB714953.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00247, whole genome shotgun sequence Length = 1787195 Score = 167 bits (422), Expect(2) = 4e-68 Identities = 77/110 (70%), Positives = 95/110 (86%) Frame = -3 Query: 221 IIIGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKVKMVVS 280 +++GGVAT +CQP+EF+M D+ V GD+LVL K LGTQ+AV HQWLD PE+WNK+K VV+ Sbjct: 588036 VVLGGVATTLCQPSEFLMLDNVVPGDMLVLMKTLGTQMAVAVHQWLDIPEKWNKIKPVVT 587857 Query: 281 REEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLA 330 +E+V+LAYQEAM NMA LNRTA+GLMH F+AH ATDIT FGILGH+QNLA Sbjct: 587856 QEDVKLAYQEAMMNMAKLNRTASGLMHMFSAHTATDITSFGILGHAQNLA 587707 Score = 117 bits (293), Expect(2) = 4e-68 Identities = 53/87 (60%), Positives = 64/87 (73%) Frame = -2 Query: 115 LGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNXX 174 LGIGMD+CVIPLRHG L LVQT D+ YP++ DPY+MGRIACANVLSDLY MG+ ECDN Sbjct: 588352 LGIGMDTCVIPLRHGSLCLVQTMDYIYPIIGDPYVMGRIACANVLSDLYTMGVMECDNML 588173 Query: 175 XXXXXXXXXXXXXREKVTPLMVKGFRD 201 R+KV PL+++GF+D Sbjct: 588172 MLFGISNKMTDRERDKVMPLIIQGFKD 588092 >gi|472269855|gb|KB714834.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00128, whole genome shotgun sequence Length = 2242837 Score = 124 bits (310), Expect = 2e-26 Identities = 62/72 (86%), Positives = 66/72 (91%), Gaps = 1/72 (1%) Frame = -1 Query: 302 AAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASGR-F 360 AAGLMHTFNAHAATDITGFGILGH+QNLAKQQRNEVSFVIHNLP++AKMAAVSKA G F Sbjct: 875617 AAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMF 875438 Query: 361 GLLQGTSAETSG 372 GL+ GT ETSG Sbjct: 875437 GLMHGTCPETSG 875402 Score = 114 bits (285), Expect = 1e-23 Identities = 54/63 (85%), Positives = 58/63 (92%) Frame = -3 Query: 372 GGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGAT 431 GGLLICLPREQAARFC+EIKS KYGEGHQAWI+GIVEKGNRTARIIDKPR+IEV P+ AT Sbjct: 873830 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVAT 873651 Query: 432 AAV 434 V Sbjct: 873650 QNV 873642 Score = 78.6 bits (192), Expect = 9e-13 Identities = 43/81 (53%), Positives = 51/81 (62%) Frame = -2 Query: 70 LKLLAGLTRPDVRPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALGIGMDSCVIPLRHG 129 + LLAGL+ P P + L+ E P G TF GIGMD+CVIPLRHG Sbjct: 894423 IALLAGLSAP*ASCP--KLLLFATLMV*NEMAFPFGFWTLTTFVFAGIGMDTCVIPLRHG 894250 Query: 130 GLSLVQTTDFFYPLVEDPYMM 150 GLSLVQTTD+ YP+V+DPYMM Sbjct: 894249 GLSLVQTTDYIYPIVDDPYMM 894187 Score = 60.5 bits (145), Expect = 3e-07 Identities = 26/36 (72%), Positives = 32/36 (88%) Frame = -3 Query: 269 PERWNKVKMVVSREEVELAYQEAMFNMATLNRTAAG 304 PE+WNK+K+VV++E+VELAYQEAM NMA LNRT G Sbjct: 884480 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTGIG 884373 Score = 60.1 bits (144), Expect = 3e-07 Identities = 26/51 (50%), Positives = 33/51 (64%) Frame = -3 Query: 188 REKVTPLMVKGFRDXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQPNEFIM 238 R+KV PL+++GF+D NPWI++GGVAT VCQPNEFIM Sbjct: 890219 RDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 890067 Score = 56.2 bits (134), Expect = 5e-06 Identities = 26/32 (81%), Positives = 27/32 (84%) Frame = -3 Query: 236 FIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLD 267 F PD+AV GDVLVLTKPLGTQVAV HQWLD Sbjct: 886592 FFRPDNAVPGDVLVLTKPLGTQVAVAVHQWLD 886497 Score = 51.6 bits (122), Expect = 1e-04 Identities = 22/26 (84%), Positives = 23/26 (88%) Frame = -2 Query: 147 PYMMGRIACANVLSDLYAMGITECDN 172 P GRIACANVLSDLYAMG+TECDN Sbjct: 891216 PLFQGRIACANVLSDLYAMGVTECDN 891139 Score = 48.1 bits (113), Expect = 0.001 Identities = 23/47 (48%), Positives = 30/47 (63%) Frame = -3 Query: 30 RSFSNYRPFEPQALGLSPSWRLTGFSGMKGUGCKVPQEALLKLLAGL 76 R+ S F P++ L S+RLT F+ +KG GCKVPQ+ L KLL L Sbjct: 899525 RTMSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESL 899385 >gi|472269647|gb|KB715042.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00336, whole genome shotgun sequence Length = 1460487 Score = 37.0 bits (84), Expect = 3.0 Identities = 28/91 (30%), Positives = 38/91 (41%), Gaps = 15/91 (16%) Frame = -2 Query: 61 GCKVPQEALLKLLAGLTRPDVRPPLGRGLVG-------------GQEEASQEAGLPA--G 105 G +PQ+ L L T P RP G G G ++ QEA P G Sbjct: 586802 GSFLPQDQLQLLSPSWTMPR-RPDSGHRKTGNRVC*GIWRRPEEGMDQPRQEADAPGLPG 586626 Query: 106 AGPSPTFPALGIGMDSCVIPLRHGGLSLVQT 136 +GP+ T P LG+G++ C+ G L T Sbjct: 586625 SGPAVTMPCLGLGLEPCLFAPSRGRTDLPLT 586533 >gi|472269897|gb|KB714792.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00086, whole genome shotgun sequence Length = 2940345 Score = 37.0 bits (84), Expect = 3.0 Identities = 24/69 (34%), Positives = 38/69 (55%) Frame = +1 Query: 319 GFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASGRFGLLQGTSAETSGGLLICL 378 G G LGH +K +R E S ++++P IAKM AVS + L+ E S G+ +C Sbjct: 2857678 GNGRLGHH---SKFRRYEPSSCLYSVPFIAKMLAVSGGKDQVHCLR----EDSLGITVCR 2857836 Query: 379 PREQAARFC 387 P++++ C Sbjct: 2857837 PKKKSRVLC 2857863 >gi|472268182|gb|KB716475.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01769, whole genome shotgun sequence Length = 562350 Score = 36.6 bits (83), Expect = 3.9 Identities = 27/66 (40%), Positives = 31/66 (46%), Gaps = 3/66 (4%) Frame = +3 Query: 86 GRGLVGGQEEASQEAGLPAGAGPSPTFP---ALGIGMDSCVIPLRHGGLSLVQTTDFFYP 142 GRG VGG+ EAGLPA AGP PT A G G D C RH G+ +P Sbjct: 201714 GRGGVGGR----LEAGLPARAGPHPTSQGPGARGHGADVC----RH*GMPRFSVNRTVFP 201869 Query: 143 LVEDPY 148 + Y Sbjct: 201870 MTRCKY 201887 >gi|472268794|gb|KB715863.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01157, whole genome shotgun sequence Length = 730242 Score = 36.2 bits (82), Expect = 5.1 Identities = 25/59 (42%), Positives = 27/59 (45%), Gaps = 3/59 (5%) Frame = +3 Query: 37 PFEPQALGLSPSWR---LTGFSGMKGUGCKVPQEALLKLLAGLTRPDVRPPLGRGLVGG 92 P+ P A S WR G G G P ALL LLA RP RPPL L+GG Sbjct: 632532 PWAPPARDSSTWWRKCSAVGCGGSAGRSSAPP--ALLHLLAPSARPAARPPLQAALLGG 632702 >gi|472266711|gb|KB717946.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold03240, whole genome shotgun sequence Length = 251378 Score = 35.8 bits (81), Expect = 6.7 Identities = 21/41 (51%), Positives = 23/41 (56%) Frame = -1 Query: 82 RPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALGIGMDSC 122 RPP RGL G E S G P GAG +PT PA G G+ C Sbjct: 112646 RPP-ARGLKGTPNEMSSWEG-PQGAGEAPTLPAGGPGLGGC 112530 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.320 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,516,518,933 Number of Sequences: 16710 Number of extensions: 22468575 Number of successful extensions: 102769 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 658 Number of HSP's successfully gapped in prelim test: 854 Number of HSP's that attempted gapping in prelim test: 93321 Number of HSP's gapped (non-prelim): 27604 length of query: 483 length of database: 1,052,295,386 effective HSP length: 136 effective length of query: 347 effective length of database: 1,050,022,826 effective search space: 364357920622 effective search space used: 364357920622 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 80 (35.4 bits)