TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000060_2.0 # Protein # Selenophosphate synthetase 1 (SPS1)
# Human (Homo sapiens)
(321 letters)
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno
me.fa
16,710 sequences; 3,156,886,159 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|472269029|gb|KB715628.1| Leptonychotes weddellii isolate WS11... 353 1e-95
gi|472269736|gb|KB714953.1| Leptonychotes weddellii isolate WS11... 194 2e-84
gi|472269855|gb|KB714834.1| Leptonychotes weddellii isolate WS11... 147 7e-34
>gi|472269029|gb|KB715628.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00922, whole genome
shotgun sequence
Length = 925757
Score = 353 bits (905), Expect = 1e-95
Identities = 179/276 (64%), Positives = 209/276 (75%), Gaps = 22/276 (7%)
Frame = +1
Query: 2 STRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESL---------------- 45
S+ F P++ L S+RLT F+ +KG GCKVPQ+ L KLL L
Sbjct: 469735 SSYRPFEPQALGLSPSWRLTGFSGMKG*GCKVPQETLLKLLAGLTRPDVRPPLGLGLGLV 469914
Query: 46 --QENHFQEDEQFLGA----VMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 99
E QE GA P LGIGMD+CVIPLRHGGLSLVQTTD+ YP+V+DPYMM
Sbjct: 469915 GGHEEAAQEGGLPAGAEPNATFPALGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM 470094
Query: 100 GRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSV 159
GRIACANVLSDLYAMG+TECDNMLMLL VS MT+ ER+K+ PL+++GF+DAAEE GT+V
Sbjct: 470095 GRIACANVLSDLYAMGITECDNMLMLLSVSQSMTEEEREKITPLMVKGFRDAAEEGGTAV 470274
Query: 160 TGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIPE 219
TGGQTV+NPWI++GGVAT VCQPNEFIMPD+AV GDVLVLTKPLGTQVAV HQWLD PE
Sbjct: 470275 TGGQTVVNPWIIVGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPE 470454
Query: 220 KWNKIKLVVTQEDVELAYQEAMMNMARLNRTGGLLI 255
+WNKI +VV++E+VELAYQEAM NMA LNRT L+
Sbjct: 470455 RWNKINMVVSREEVELAYQEAMFNMATLNRTAAGLM 470562
Score = 114 bits (285), Expect = 8e-24
Identities = 53/61 (86%), Positives = 58/61 (95%)
Frame = +1
Query: 250 TGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVA 309
+GGLLICLPREQAARFC+EIKS KYGEGHQAWI+GIVEKGNRTARIIDKPR+IEV P+ A
Sbjct: 470755 SGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGA 470934
Query: 310 T 310
T
Sbjct: 470935 T 470937
>gi|472269736|gb|KB714953.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00247, whole genome
shotgun sequence
Length = 1787195
Score = 194 bits (492), Expect(2) = 2e-84
Identities = 93/105 (88%), Positives = 97/105 (92%)
Frame = -2
Query: 64 LGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNML 123
LGIGMDTCVIPLRHG L LVQT DYIYPI+ DPY+MGRIACANVLSDLY MGV ECDNML
Sbjct: 588352 LGIGMDTCVIPLRHGSLCLVQTMDYIYPIIGDPYVMGRIACANVLSDLYTMGVMECDNML 588173
Query: 124 MLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNP 168
ML G+SNKMTDRERDKVMPLIIQGFKDAA+EAGTSVTGGQ VLNP
Sbjct: 588172 MLFGISNKMTDRERDKVMPLIIQGFKDAAKEAGTSVTGGQMVLNP 588038
Score = 143 bits (361), Expect(2) = 2e-84
Identities = 69/86 (80%), Positives = 78/86 (90%)
Frame = -3
Query: 170 IVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVT 229
+VLGGVATT+CQP+EF+M DN VPGD+LVL K LGTQ+AVAVHQWLDIPEKWNKIK VVT
Sbjct: 588036 VVLGGVATTLCQPSEFLMLDNVVPGDMLVLMKTLGTQMAVAVHQWLDIPEKWNKIKPVVT 587857
Query: 230 QEDVELAYQEAMMNMARLNRTGGLLI 255
QEDV+LAYQEAMMNMA+LNRT L+
Sbjct: 587856 QEDVKLAYQEAMMNMAKLNRTASGLM 587779
>gi|472269855|gb|KB714834.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00128, whole genome
shotgun sequence
Length = 2242837
Score = 147 bits (372), Expect = 7e-34
Identities = 71/71 (100%), Positives = 71/71 (100%)
Frame = -3
Query: 251 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVAT 310
GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVAT
Sbjct: 873830 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVAT 873651
Query: 311 QNVNPTPGATS 321
QNVNPTPGATS
Sbjct: 873650 QNVNPTPGATS 873618
Score = 134 bits (337), Expect = 8e-30
Identities = 65/66 (98%), Positives = 66/66 (100%)
Frame = -3
Query: 1 MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAV 60
MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAV
Sbjct: 899519 MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAV 899340
Query: 61 MPRLGI 66
MPRLG+
Sbjct: 899339 MPRLGM 899322
Score = 108 bits (270), Expect = 4e-22
Identities = 52/52 (100%), Positives = 52/52 (100%)
Frame = -3
Query: 136 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 187
ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM
Sbjct: 890222 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 890067
Score = 78.2 bits (191), Expect = 6e-13
Identities = 35/35 (100%), Positives = 35/35 (100%)
Frame = -2
Query: 65 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 99
GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM
Sbjct: 894291 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 894187
Score = 77.8 bits (190), Expect = 8e-13
Identities = 36/40 (90%), Positives = 37/40 (92%)
Frame = -2
Query: 96 PYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDR 135
P GRIACANVLSDLYAMGVTECDNMLMLLG+SNKMTDR
Sbjct: 891216 PLFQGRIACANVLSDLYAMGVTECDNMLMLLGISNKMTDR 891097
Score = 72.0 bits (175), Expect = 5e-11
Identities = 34/34 (100%), Positives = 34/34 (100%)
Frame = -3
Query: 218 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTG 251
PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTG
Sbjct: 884480 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTG 884379
Score = 67.0 bits (162), Expect = 1e-09
Identities = 31/33 (93%), Positives = 31/33 (93%)
Frame = -3
Query: 185 FIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 217
F PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI
Sbjct: 886592 FFRPDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 886494
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge
nome.fa
Posted date: Oct 18, 2013 6:11 PM
Number of letters in database: 3,087,506,849
Number of sequences in database: 5788
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge
nome.fa.01
Posted date: Oct 18, 2013 6:11 PM
Number of letters in database: 69,379,310
Number of sequences in database: 10,922
Lambda K H
0.319 0.136 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,135,332,343
Number of Sequences: 16710
Number of extensions: 16578773
Number of successful extensions: 54829
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 373
Number of HSP's successfully gapped in prelim test: 292
Number of HSP's that attempted gapping in prelim test: 52363
Number of HSP's gapped (non-prelim): 7856
length of query: 321
length of database: 1,052,295,386
effective HSP length: 132
effective length of query: 189
effective length of database: 1,050,089,666
effective search space: 198466946874
effective search space used: 198466946874
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 77 (34.3 bits)