Command line: [exonerate -m p2g --showtargetgff -q ./SBP2.fa -t SBP2.carpeta/genomic.fa] Hostname: [ws101071] C4 Alignment: ------------ Query: SPP00000054_2.0 # Protein # SECIS binding protein 2 (SBP2) # Human (Homo sapiens) Target: gi|472267807|gb|KB716850.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02144, whole genome shotgun sequence Model: protein2genome:local Raw score: 1309 Query range: 473 -> 840 Target range: 336473 -> 365899 474 : ProValValValSerValGlyAlaValProValLeuSerLysGluCysAlaSerGlyG : 493 |||:!! !|||:!!||||||||||||||||||||||||!:!!!: !|||||||||: ProLeuArgValAlaValGlyAlaValProValLeuSerArgAspThrAlaSerGlyL 336474 : CCCCTCCGTGTCGCAGTCGGGGCGGTGCCGGTCCTCTCCAGAGACACCGCGTCGGGGA : 336531 494 : luArgGlyArgArgMetSerGlnMetLysThrProHisAsnProLeuAspSerSerAl : 512 !!!:!|||!.!!.!:!!||||||:!!|||||||||||||||||||||||||||||||| ysLysGlyHisHisLeuSerGlnValLysThrProHisAsnProLeuAspSerSerAl 336532 : AGAAGGGCCACCACTTGAGCCAGGTGAAGACCCCGCACAACCCCTTGGACTCCAGCGC : 336588 513 : aProLeuMetLysLysGlyLysGlnArgGluIleProLysAlaLysLysProThrSer : 531 |||||||||||||||||||||||||||||||:!!|||||||||||||||||||||||| aProLeuMetLysLysGlyLysGlnArgGluValProLysAlaLysLysProThrSer 336589 : CCCACTGATGAAGAAGGGGAAGCAGAGGGAGGTCCCCAAGGCCAAGAAGCCAACCTCG : 336645 532 : LeuLysLys >>>> Target Intron 1 >>>> IleIleLeuLysGluArgGl : 541 ||||||||| 570 bp |||||||||||||||||||| LeuLysLys++ ++IleIleLeuLysGluArgGl 336646 : TTGAAGAAGgt.........................agATTATTCTGAAAGAACGGCA : 337245 542 : nGluArgLys<->GlnArgLeuGlnGluAsnAlaValSerProAlaPheThrSerAsp : 559 |||||||||| |||||||||||||||||||||||||||||||||.!!.!!|||||| nGluArgLysGlnGlnArgLeuGlnGluAsnAlaValSerProAlaLeuAlaSerAsp 337246 : GGAGAGAAAGCAGCAGCGTCTCCAAGAAAATGCCGTGAGCCCAGCTCTCGCCAGTGAT : 337302 560 : AspThrGlnAspGlyGluSerGlyGlyAspAspGlnPheProGluGlnAlaGluLeu{ : 579 ! !..!! !|||||||||||||||||||||!!: .!! ! ! ! ! ! !{ AlaValProAspGlyGluSerGlyGlyAspGluAlaLeuGluProAlaAspProSer{ 337303 : GCTGTACCGGATGGTGAGAGCGGTGGTGACGAGGCCCTCGAGCCGGCCGACCCCTCA{ : 337362 580 : S} >>>> Target Intron 2 >>>> {er}GlyProGluGlyMetAspGluLe : 587 .} 586 bp {!!}|||||||||!.! ! G}++ ++{ly}GlyProGluAlaSerPro----- 337363 : G}gt.........................ag{GA}GGGCCGGAGGCGTCGCCG----- : 337969 588 : uIleSerThrProSerValGluAspLysSerGluGluProProGlyThrGluLeuGln : 606 !|||.!!|||! !:!!|||! !!:!|||||||||! !||| !!.!!||||||||| -CysSerAlaProMetLeuGluGlyArgSerGluGluLeuProArgAlaGluLeuGln 337970 : -TGCTCTGCGCCCATGCTGGAGGGCAGGTCAGAAGAGCTGCCGAGAGCGGAGCTCCAG : 338023 607 : ArgAspThrGluAlaSerHisLeuAlaProAsnHisThrThrPheProLysIleHisS : 626 !:!!!:..!|||!.!!!!||||||!.!|||||| !.!! !! !||||||||||||| LysGluValGluGlyCysHisLeuValProAsnGlyAlaGlyCysProLysIleHisS 338024 : AAGGAGGTAGAGGGCTGCCACCTCGTTCCCAACGGCGCTGGCTGCCCCAAGATCCACA : 338083 627 : erArgArgPheArg{As} >>>> Target Intron 3 >>>> {p}TyrCysSe : 634 ||||||||||||||{||} 510 bp {|}|||||||| erArgArgPheArg{As}++ ++{p}TyrCysSe 338084 : GCCGGAGATTCCGG{GA}gt.........................ag{T}TACTGCAG : 338617 635 : rGlnMetLeuSerLysGluValAspAlaCysValThrAspLeuLeuLysGluLeuVal : 653 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| rGlnMetLeuSerLysGluValAspAlaCysValThrAspLeuLeuLysGluLeuVal 338618 : CCAGATGCTGAGTAAGGAGGTGGACGCCTGTGTGACGGATCTGCTGAAGGAGCTGGTG : 338674 654 : ArgPheGlnAspArgMetTyrGlnLysAspProValLysAlaLysThrLysArgArgL : 673 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ArgPheGlnAspArgMetTyrGlnLysAspProValLysAlaLysThrLysArgArgL 338675 : CGCTTCCAAGACCGCATGTACCAGAAAGATCCAGTCAAGGCTAAGACCAAACGCCGAC : 338734 674 : euValLeuGlyLeuArgGluValLeuLysHisLeuLysLeuLysLysLeuLysCysVa : 692 |||||||||||||||||||||||||||||||||||||||||!:!||||||||||||:! euValLeuGlyLeuArgGluValLeuLysHisLeuLysLeuArgLysLeuLysCysIl 338735 : TCGTGCTGGGGCTGCGGGAGGTCCTCAAGCATCTGAAGCTCAGGAAACTCAAGTGCAT : 338791 693 : lIleIleSerProAsnCysGluLysIleGlnSerLys{G} >>>> Target Intr : 705 !||||||||||||||||||||||||||||||||||||{|} 5346 bp eIleIleSerProAsnCysGluLysIleGlnSerLys{G}++ 338792 : CATCATCTCTCCCAACTGTGAGAAGATCCAGTCGAAA{G}gt................ : 338833 706 : on 4 >>>> {ly}GlyLeuAspAspThrLeuHisThrIleIleAspTyrAlaCysG : 720 {||}||||||||||||||||||||||||||||||||||||||||||| ++{ly}GlyLeuAspAspThrLeuHisThrIleIleAspTyrAlaCysG 338834 : .........ag{GT}GGGCTGGACGACACGCTGCACACCATCATCGATTACGCCTGTG : 344221 721 : luGlnAsnIleProPheValPheAlaLeuAsnArgLysAlaLeuGlyArgSerLeuAs : 739 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| luGlnAsnIleProPheValPheAlaLeuAsnArgLysAlaLeuGlyArgSerLeuAs 344222 : AGCAGAATATTCCCTTCGTGTTCGCGCTCAACCGCAAAGCTCTGGGGCGCAGTTTGAA : 344278 740 : nLysAlaValProValSerValValGlyIlePheSerTyrAspGlyAlaGln >>>> : 757 |||||||||||||||||||||||||||||||||||||||||||||||||||| nLysAlaValProValSerValValGlyIlePheSerTyrAspGlyAlaGln++ 344279 : CAAGGCCGTTCCCGTCAGCGTGGTGGGCATCTTCAGCTACGACGGGGCCCAGgt.... : 344334 758 : Target Intron 5 >>>> AspGlnPheHisLysMetValGluLeuThrValAl : 768 146 bp ||||||||||||!:!|||||||||||||||:!!|| ++AspGlnPheHisArgMetValGluLeuThrMetAl 344335 : .....................agGACCAGTTCCACAGGATGGTCGAGCTGACGATGGC : 344511 769 : aAlaArgGlnAlaTyrLysThrMetLeuGluAsnValGlnGlnGluLeuValGlyGlu : 787 |||||||||||||||||||!:!|||||||||||||||!::!:!||||||!.!|||||| aAlaArgGlnAlaTyrLysSerMetLeuGluAsnValArgArgGluLeuAlaGlyGlu 344512 : CGCCCGGCAGGCGTACAAGAGCATGCTGGAGAACGTGCGCCGGGAGCTGGCTGGGGAG : 344568 788 : ProArgProGlnAlaProProSerLeuProThrGlnGlyProSerCysProAlaGluA : 807 ||| !!|||! !|||||| !!|||! !|||..!|||||||||.!! !! !!|||:!!| ProGlyProProAlaProAlaSerProProValGlnGlyProGlyGlySerAlaGlnA 344569 : CCTGGGCCCCCGGCTCCGGCCAGCCCGCCCGTGCAGGGCCCCGGCGGCTCGGCGCAGG : 344628 808 : spGlyProProAla<->LeuLysGluLysGluGluProHisTyrIleGluIleTrpLy : 825 |||||||||||||| ! ! !!..|||||||||:!!|||! !||| !||| spGlyProProAlaProAlaProGlySerGluGluProTyrTyrSerGluCysTrpGl 344629 : ACGGCCCCCCTGCCCCTGCACCCGGGAGCGAAGAGCCTTACTACAGTGAGTGCTGGGG : 344685 826 : s{L} >>>> Target Intron 6 >>>> {ys}HisLeuGluAlaTyrSerGly : 833 !{ } 21167 bp { !}|||:!!|||.!! !!:!!! ! y{G}+- ++{ly}HisValGluThrAspAlaVal 344686 : A{G}gg.........................ag{GA}CACGTAGAAACTGATGCAGTC : 365876 834 : CysThrLeuGluLeuGluGlu : 840 ||||||||||||:!!|||:!! CysThrLeuGluValGluLys 365877 : TGCACTTTGGAGGTGGAAAAG : 365899 vulgar: SPP00000054_2.0 473 840 . gi|472267807|gb|KB716850.1| 336473 365899 + 1309 M 61 183 5 0 2 I 0 566 3 0 2 M 10 30 G 0 3 M 34 102 S 0 1 5 0 2 I 0 582 3 0 2 S 1 2 M 6 18 G 2 0 M 43 129 S 0 2 5 0 2 I 0 506 3 0 2 S 1 1 M 73 219 S 0 1 5 0 2 I 0 5342 3 0 2 S 1 2 M 51 153 5 0 2 I 0 142 3 0 2 M 55 165 G 0 3 M 14 42 S 0 1 5 0 2 I 0 21163 3 0 2 S 1 2 M 14 42 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472267807|gb|KB716850.1| exonerate:protein2genome:local gene 336474 365899 1309 + . gene_id 1 ; sequence SPP00000054_2.0 ; gene_orientation + gi|472267807|gb|KB716850.1| exonerate:protein2genome:local cds 336474 336656 . + . gi|472267807|gb|KB716850.1| exonerate:protein2genome:local exon 336474 336656 . + . insertions 0 ; deletions 0 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice5 336657 336658 . + . intron_id 1 ; splice_site "GT" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local intron 336657 337226 . + . intron_id 1 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice3 337225 337226 . + . intron_id 0 ; splice_site "AG" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local cds 337227 337362 . + . gi|472267807|gb|KB716850.1| exonerate:protein2genome:local exon 337227 337362 . + . insertions 3 ; deletions 0 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice5 337363 337364 . + . intron_id 2 ; splice_site "GT" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local intron 337363 337948 . + . intron_id 2 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice3 337947 337948 . + . intron_id 1 ; splice_site "AG" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local cds 337949 338099 . + . gi|472267807|gb|KB716850.1| exonerate:protein2genome:local exon 337949 338099 . + . insertions 0 ; deletions 2 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice5 338100 338101 . + . intron_id 3 ; splice_site "GT" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local intron 338100 338609 . + . intron_id 3 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice3 338608 338609 . + . intron_id 2 ; splice_site "AG" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local cds 338610 338830 . + . gi|472267807|gb|KB716850.1| exonerate:protein2genome:local exon 338610 338830 . + . insertions 0 ; deletions 0 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice5 338831 338832 . + . intron_id 4 ; splice_site "GT" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local intron 338831 344176 . + . intron_id 4 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice3 344175 344176 . + . intron_id 3 ; splice_site "AG" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local cds 344177 344331 . + . gi|472267807|gb|KB716850.1| exonerate:protein2genome:local exon 344177 344331 . + . insertions 0 ; deletions 0 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice5 344332 344333 . + . intron_id 5 ; splice_site "GT" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local intron 344332 344477 . + . intron_id 5 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice3 344476 344477 . + . intron_id 4 ; splice_site "AG" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local cds 344478 344688 . + . gi|472267807|gb|KB716850.1| exonerate:protein2genome:local exon 344478 344688 . + . insertions 3 ; deletions 0 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice5 344689 344690 . + . intron_id 6 ; splice_site "GG" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local intron 344689 365855 . + . intron_id 6 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local splice3 365854 365855 . + . intron_id 5 ; splice_site "AG" gi|472267807|gb|KB716850.1| exonerate:protein2genome:local cds 365856 365899 . + . gi|472267807|gb|KB716850.1| exonerate:protein2genome:local exon 365856 365899 . + . insertions 0 ; deletions 0 gi|472267807|gb|KB716850.1| exonerate:protein2genome:local similarity 336474 365899 1309 + . alignment_id 1 ; Query SPP00000054_2.0 ; Align 336474 474 183 ; Align 337227 535 30 ; Align 337260 545 102 ; Align 337951 580 18 ; Align 337969 588 129 ; Align 338611 632 219 ; Align 344179 706 153 ; Align 344478 757 165 ; Align 344646 812 42 ; Align 365858 827 42 # --- END OF GFF DUMP --- # -- completed exonerate analysis