TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000054_2.0 # Protein # SECIS binding protein 2 (SBP2) # Human (Homo sapiens) (854 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472267807|gb|KB716850.1| Leptonychotes weddellii isolate WS11... 127 5e-27 gi|472267499|gb|KB717158.1| Leptonychotes weddellii isolate WS11... 112 2e-22 gi|472269286|gb|KB715371.1| Leptonychotes weddellii isolate WS11... 104 2e-20 gi|472267563|gb|KB717094.1| Leptonychotes weddellii isolate WS11... 37 8.1 gi|472269699|gb|KB714990.1| Leptonychotes weddellii isolate WS11... 37 8.1 >gi|472267807|gb|KB716850.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02144, whole genome shotgun sequence Length = 429869 Score = 127 bits (318), Expect = 5e-27 Identities = 63/74 (85%), Positives = 64/74 (86%) Frame = +1 Query: 632 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVXXXXXXXXXXC 691 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREV C Sbjct: 338611 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKC 338790 Query: 692 VIISPNCEKIQSKG 705 +IISPNCEKIQSKG Sbjct: 338791 IIISPNCEKIQSKG 338832 Score = 107 bits (268), Expect = 3e-21 Identities = 52/52 (100%), Positives = 52/52 (100%) Frame = +1 Query: 705 GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 756 GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ Sbjct: 344176 GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 344331 Score = 97.8 bits (242), Expect = 3e-18 Identities = 53/98 (54%), Positives = 63/98 (64%), Gaps = 1/98 (1%) Frame = +3 Query: 728 ALNRKALGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAARQAYKTMLENVQQELVGE 787 AL+ A G + +P V QDQFH+MVELT+AARQAYK+MLENV++EL GE Sbjct: 344391 ALSLGAAGWKAGRRIPWGRVSPVRSLLPQDQFHRMVELTMAARQAYKSMLENVRRELAGE 344570 Query: 788 PRPQAPPSLPTQGPSCPAEDGPPA-LKEKEEPHYIEIW 824 P P AP S P QGP A+DGPPA EEP+Y E W Sbjct: 344571 PGPPAPASPPVQGPGGSAQDGPPAPAPGSEEPYYSECW 344684 Score = 85.5 bits (210), Expect = 2e-14 Identities = 38/48 (79%), Positives = 45/48 (93%) Frame = +3 Query: 488 ECASGERGRRMSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKI 535 + ASG++G +SQ+KTPHNPLDSSAPLMKKGKQRE+PKAKKPTSLKK+ Sbjct: 336516 DTASGKKGHHLSQVKTPHNPLDSSAPLMKKGKQREVPKAKKPTSLKKV 336659 Score = 62.0 bits (149), Expect = 2e-07 Identities = 36/50 (72%), Positives = 38/50 (76%), Gaps = 1/50 (2%) Frame = +3 Query: 534 KIILKERQERKQ-RLQENAVSPAFTSDDTQDGESGGDDQFPEQAELSGPE 582 KIILKERQERKQ RLQENAVSPA SD DGESGGD+ E A+ SG E Sbjct: 337224 KIILKERQERKQQRLQENAVSPALASDAVPDGESGGDEAL-EPADPSGAE 337370 Score = 54.7 bits (130), Expect = 3e-05 Identities = 35/95 (36%), Positives = 45/95 (47%), Gaps = 3/95 (3%) Frame = +1 Query: 540 RQERKQRLQENAVSPAF---TSDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDK 596 R+ R L+ V PA+ D G +GG + P S P +E + Sbjct: 337858 REVRSGLLRPQPVCPAWGRLCPDSLLRGVAGGPEASP--------------CSAPMLEGR 337995 Query: 597 SEEPPGTELQRDTEASHLAPNHTTFPKIHSRRFRD 631 SEE P ELQ++ E HL PN PKIHSRRFR+ Sbjct: 337996 SEELPRAELQKEVEGCHLVPNGAGCPKIHSRRFRE 338100 >gi|472267499|gb|KB717158.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02452, whole genome shotgun sequence Length = 366617 Score = 112 bits (279), Expect = 2e-22 Identities = 53/87 (60%), Positives = 64/87 (73%) Frame = +2 Query: 57 PPVTEQKIYTEDMAFGASTFPPQYLSSEITLHPYAYSPYTLDSTQNVYSVPGSQYLYNQP 116 P + QK+ TED+AFGAS F PQYLSSE+ LHP YSPY ++ +Q+ VPGSQY +P Sbjct: 34637 PVLFRQKLCTEDVAFGASPFLPQYLSSELALHPCRYSPYPVECSQSGCPVPGSQYACGRP 34816 Query: 117 SCYRGFQTVKHRNENTCPLPQEMKALF 143 S YRGFQTVK R E CP+PQ+ KALF Sbjct: 34817 SAYRGFQTVKPRGEQMCPVPQDSKALF 34897 Score = 107 bits (268), Expect = 3e-21 Identities = 55/92 (59%), Positives = 71/92 (77%), Gaps = 2/92 (2%) Frame = +2 Query: 178 SHHLSIYAE-NSLKSDGYHKRTDRKSRIIAKNVSTSKPEFEFTTLDFPELQGAENN-MSE 235 SH+ ++ +S+ +DGY KRTDRKS I+ K+ S SKPEFEFT LDFPELQG E++ +SE Sbjct: 36365 SHNTYLFTPFDSVNTDGYPKRTDRKSGIVEKSGSASKPEFEFTRLDFPELQGPEHSEISE 36544 Query: 236 IQKQPKWGPVHSVSTDISLLREVVKPAAVLSK 267 +KQ KWGP+ S S D+SLLREVVKPA V ++ Sbjct: 36545 TEKQSKWGPLRSTSADLSLLREVVKPAVVTTE 36640 Score = 102 bits (254), Expect = 1e-19 Identities = 55/70 (78%), Positives = 60/70 (85%) Frame = +2 Query: 294 ELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPSYNK 353 ELSWTPMGYVVRQTLSTE SAAPKNVTSMINL+T+ASSAD K+VS+ E LSSDPSY K Sbjct: 41357 ELSWTPMGYVVRQTLSTEQSAAPKNVTSMINLETVASSADSKSVSMSPPEVLSSDPSY-K 41533 Query: 354 EKHIIHPTQK 363 EKH IH +K Sbjct: 41534 EKH-IHSAKK 41560 Score = 79.7 bits (195), Expect = 8e-13 Identities = 39/50 (78%), Positives = 41/50 (82%) Frame = +3 Query: 12 EGIKLSADVKPFVPRFAGLNVAWLESSEACVFPSSAATYYPFVQEPPVTE 61 +G KLSADVKPFVP+FAGL VAW ESSEA VFPS AA YYP VQE PV E Sbjct: 30072 QGFKLSADVKPFVPKFAGLGVAWSESSEARVFPSCAAAYYPCVQELPVPE 30221 Score = 59.3 bits (142), Expect = 1e-06 Identities = 28/34 (82%), Positives = 31/34 (91%) Frame = +1 Query: 435 DNFKNNVKKSQLPVQLDLGGMLTALEKKQHSQHA 468 +NFKN+ KKSQ+PVQLDLGGML ALEKKQHSQ A Sbjct: 46735 NNFKNSGKKSQIPVQLDLGGMLAALEKKQHSQSA 46836 Score = 56.6 bits (135), Expect = 8e-06 Identities = 26/34 (76%), Positives = 30/34 (88%) Frame = +3 Query: 405 DAEEFPNLAVASERRDRIETPKFQSKQQPQDNFK 438 DAEEFPNLAVASERRDR+ +P FQS+QQPQ F+ Sbjct: 45375 DAEEFPNLAVASERRDRVASPNFQSQQQPQVIFR 45476 Score = 50.8 bits (120), Expect = 4e-04 Identities = 26/50 (52%), Positives = 37/50 (74%) Frame = +2 Query: 156 QQKFDSERADGTISSEIKSARGSHHLSIYAENSLKSDGYHKRTDRKSRII 205 +QKFDS++ADG++SS+IKS RGSH +SI+A+ +LK DR S +I Sbjct: 35138 EQKFDSKKADGSLSSDIKSGRGSHPVSIHADANLKPG----ENDRLSDVI 35275 Score = 47.0 bits (110), Expect = 0.006 Identities = 25/42 (59%), Positives = 26/42 (61%) Frame = +3 Query: 363 KSKASQGSDLEQNEAXXXXXXXXXXXXXXYEVLTVQEPPRIE 404 +SKASQG D EQNEA YEVLTVQEPPRIE Sbjct: 44034 ESKASQGGDPEQNEASRKHKKKKEKSKSKYEVLTVQEPPRIE 44159 Score = 40.4 bits (93), Expect = 0.56 Identities = 24/60 (40%), Positives = 30/60 (50%), Gaps = 1/60 (1%) Frame = +2 Query: 796 LPTQG-PSCPAEDGPPALKEKEEPHYIEIWKKHLEAYSGCXXXXXXXXXXXXXQMMNLNL 854 LP++G P CP +L +EIW++HLEAYS C QMMNLNL Sbjct: 78620 LPSRGCPDCPRV----SLSCATLSFLVEIWRRHLEAYSRCALELEDSLEASTSQMMNLNL 78787 >gi|472269286|gb|KB715371.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00665, whole genome shotgun sequence Length = 1170685 Score = 104 bits (260), Expect = 2e-20 Identities = 50/74 (67%), Positives = 59/74 (79%) Frame = -2 Query: 632 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVXXXXXXXXXXC 691 YC+Q+L KE+D CVT LL+ELV FQ+R+YQKDPV+AK +RRLV+GLREV C Sbjct: 76068 YCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKC 75889 Query: 692 VIISPNCEKIQSKG 705 VIISPNCEKIQSKG Sbjct: 75888 VIISPNCEKIQSKG 75847 Score = 74.3 bits (181), Expect = 4e-11 Identities = 38/62 (61%), Positives = 47/62 (75%) Frame = -2 Query: 705 GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQDQFHKMVE 764 GGLD+ L+ +I A EQ IPFVFAL RKALGR +NK VPVSVVGIF+Y GA+ + ++ Sbjct: 75138 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAEVRPKRIHS 74959 Query: 765 LT 766 LT Sbjct: 74958 LT 74953 Score = 39.3 bits (90), Expect = 1.2 Identities = 18/29 (62%), Positives = 23/29 (79%) Frame = -2 Query: 442 KKSQLPVQLDLGGMLTALEKKQHSQHAKQ 470 KK++ PVQLDLG ML ALEK+Q + A+Q Sbjct: 93738 KKNKTPVQLDLGDMLAALEKQQQAMKARQ 93652 Score = 36.6 bits (83), Expect = 8.1 Identities = 16/33 (48%), Positives = 24/33 (72%) Frame = -3 Query: 503 TPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKI 535 T + LD ++ KKGK++EI K K+PT+LKK+ Sbjct: 89795 TSFSSLDITSSKAKKGKEKEIAKLKRPTALKKV 89697 >gi|472267563|gb|KB717094.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02388, whole genome shotgun sequence Length = 402887 Score = 36.6 bits (83), Expect = 8.1 Identities = 16/27 (59%), Positives = 17/27 (62%) Frame = -1 Query: 786 GEPRPQAPPSLPTQGPSCPAEDGPPAL 812 GEP P A P P QGPS PA+ G P L Sbjct: 354395 GEPPPPALPQAPRQGPSLPADSGGPPL 354315 >gi|472269699|gb|KB714990.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00284, whole genome shotgun sequence Length = 1719621 Score = 36.6 bits (83), Expect = 8.1 Identities = 18/51 (35%), Positives = 22/51 (43%) Frame = +1 Query: 44 PSSAATYYPFVQEPPVTEQKIYTEDMAFGASTFPPQYLSSEITLHPYAYSP 94 P YYP++ PP T KI + G STF PQY + H P Sbjct: 148069 PELIHAYYPYLLPPPATSNKIACK*YPLGPSTFCPQYAFCNASSHSSLLPP 148221 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.311 0.128 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 2,735,348,304 Number of Sequences: 16710 Number of extensions: 38486105 Number of successful extensions: 177289 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 932 Number of HSP's successfully gapped in prelim test: 799 Number of HSP's that attempted gapping in prelim test: 158281 Number of HSP's gapped (non-prelim): 52492 length of query: 854 length of database: 1,052,295,386 effective HSP length: 141 effective length of query: 713 effective length of database: 1,049,939,276 effective search space: 748606703788 effective search space used: 748606703788 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 82 (36.2 bits)