Command line: [exonerate -m p2g --showtargetgff -q ./PSTK.fa -t PSTK.carpeta/genomic.fa] Hostname: [ws101071] C4 Alignment: ------------ Query: SPP00000052_2.0 # Protein # Phosphoseryl-tRNA kinase (PSTK) # Human (Homo sapiens) Target: gi|472269467|gb|KB715222.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00516, whole genome shotgun sequence Model: protein2genome:local Raw score: 1258 Query range: 0 -> 293 Target range: 827354 -> 833729 1 : MetLysThrAlaGluAsnIleArgGlyThrGlySerAspGlyProArgLysArgGlyL : 20 |||||||||||||||:!! !||||||.!! !!|||!!:|||||||||:!!! !|||| MetLysThrAlaGluAspAlaArgGlyAlaArgSerGluGlyProArgGluIleGlyL 827355 : ATGAAGACCGCCGAGGACGCCAGAGGGGCGCGCAGCGAGGGACCGCGGGAGATAGGCC : 827412 21 : euCysValLeuCysGlyLeuProAlaAlaGlyLysSerThrPheAlaArgAlaLeuAl : 39 |||||:!!|||||||||||||||||||||||||||||||||!!.||||||||||||:: euCysLeuLeuCysGlyLeuProAlaAlaGlyLysSerThrLeuAlaArgAlaLeuSe 827413 : TCTGCCTTCTCTGCGGCCTCCCGGCAGCAGGAAAATCGACCTTGGCGCGGGCCCTCAG : 827469 40 : aHisArgLeuGlnGlnGluGlnGlyTrpAlaIleGlyValValAlaTyrAspAspVal : 58 !|||||||||!:!||||||!:!|||||||||:!!|||||||||.!!|||||||||||| rHisArgLeuArgGlnGluArgGlyTrpAlaValGlyValValThrTyrAspAspVal 827470 : CCACCGGCTACGGCAGGAGCGGGGCTGGGCCGTCGGCGTCGTCACCTATGATGACGTC : 827526 59 : MetProAspAlaPheLeuAlaGlyAlaArgAlaArgProAla >>>> Target In : 73 ||||||||||||||||||! !! !|||!!!||||||||| ! 2004 MetProAspAlaPheLeuGluGluAlaSerAlaArgProLeu++ 827527 : ATGCCGGACGCGTTCCTGGAGGAGGCGAGCGCGCGGCCATTGgt.............. : 827573 74 : tron 1 >>>> ProSerGlnTrpLysLeuLeuArgGlnGluLeuLeuLysTyrLeu : 87 bp ||||||||||||||||||||||||||||||||||||||||||||| ++ProSerGlnTrpLysLeuLeuArgGlnGluLeuLeuLysTyrLeu 827574 : ...........agCCATCCCAGTGGAAATTGCTTCGACAGGAACTGTTGAAATACCTA : 829617 88 : GluTyrPheLeuMetAlaValIleAsnGlyCysGlnMetSerValProProAsnArgT : 107 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GluTyrPheLeuMetAlaValIleAsnGlyCysGlnMetSerValProProAsnArgT 829618 : GAATACTTCTTGATGGCTGTCATTAACGGGTGTCAGATGTCTGTCCCACCCAACAGGA : 829677 108 : hrGluAlaMetTrpGluAspPheIleThrCysLeuLysAspGlnAspLeuIlePheSe : 126 ||! !|||:!!|||||||||||||||!:!||||||||||||||||||||||||! !|| hrAlaAlaLeuTrpGluAspPheIleSerCysLeuLysAspGlnAspLeuIleSerSe 829678 : CTGCAGCCCTGTGGGAGGATTTTATAAGCTGCTTAAAGGATCAAGATCTGATATCTTC : 829734 127 : rAlaAlaPheGluAlaGlnSerCysTyrLeuLeuThrLysThrAlaValSerArgPro : 145 |||||||.!.|||.!!||||||||||||||||||||||||! !||||||||||||||| rAlaAlaLeuGluThrGlnSerCysTyrLeuLeuThrLysIleAlaValSerArgPro 829735 : TGCGGCGCTGGAGACCCAGTCTTGCTACCTCTTAACGAAGATTGCTGTTTCTAGACCT : 829791 146 : LeuPheLeuValLeuAspAspAsnPheTyrTyrGlnSerMetArgTyrGluValTyrG : 165 |||||||||:!!|||||||||||||||||||||||||||||||||||||||||||||| LeuPheLeuIleLeuAspAspAsnPheTyrTyrGlnSerMetArgTyrGluValTyrG 829792 : TTGTTTTTGATTTTAGATGACAACTTTTATTATCAAAGTATGAGATACGAAGTCTACC : 829851 166 : lnLeuAlaArgLys{T} >>>> Target Intron 2 >>>> {yr}SerLeuGl : 173 ||||||||||||||{|} 238 bp {||}|||||||| lnLeuAlaArgLys{T}++ ++{yr}SerLeuGl 829852 : AACTGGCTCGGAAA{T}gt.........................ag{AT}TCATTAGG : 830113 174 : yPheCysGlnLeuPheLeuAspCysProLeuGluThrCysLeuGlnArgAsnGlyGln : 192 ||||||||||||||||||||||||| !!|||||||||||||||||||||||||||||| yPheCysGlnLeuPheLeuAspCysSerLeuGluThrCysLeuGlnArgAsnGlyGln 830114 : CTTTTGCCAGCTCTTTTTAGATTGTTCTCTCGAGACCTGTTTACAGAGGAATGGCCAG : 830170 193 : ArgProGlnAlaLeuProProGluThrIleHisLeuMetGlyArgLysLeuGluLysP : 212 ||| !||| !!|||||| !!||||||||||||||||||!.!||||||:!!||||||| ArgCysGlnProLeuProAlaGluThrIleHisLeuMetAlaArgLysIleGluLysP 830171 : AGATGCCAGCCACTGCCTGCCGAGACCATTCACCTGATGGCAAGAAAGATAGAGAAGC : 830230 213 : roAsnProGluLysAsnAlaTrpGluHisAsnSerLeuThrIleProSerProAlaCy : 231 ||||||||! !|||||||||||||||||||||||||||||||||! !||| !! !!|| roAsnProGlyLysAsnAlaTrpGluHisAsnSerLeuThrIleGlnSerThrProCy 830231 : CCAACCCTGGGAAAAATGCTTGGGAACACAACAGCCTCACAATTCAGAGTACCCCGTG : 830287 232 : sAlaSerGluAla{Se} >>>> Target Intron 3 >>>> {r}LeuGluVal : 239 |:!!|||||||||{||} 2759 bp {|}|||:!!:!! sSerSerGluAla{Se}++ ++{r}LeuLysLeu 830288 : CTCTTCTGAGGCC{AG}gt.........................ag{C}CTGAAGTTG : 833070 240 : ThrAspLeuLeuLeuThrAlaLeuGluAsnProValLysTyrAlaGluAspAsnMetG : 259 |||||||||||||||||||||||||||||||||||||||||| !|||||||||:!!| ThrAspLeuLeuLeuThrAlaLeuGluAsnProValLysTyrIleGluAspAsnValG 833071 : ACTGATTTATTGCTTACTGCTTTGGAAAATCCAGTAAAATATATTGAGGACAATGTGG : 833130 260 : luGlnLys >>>> Target Intron 4 >>>> AspThrAspArgIleIleCys : 268 |||||||| 495 bp !!::!!||||||||||||||| luGlnLys++ ++GluSerAspArgIleIleCys 833131 : AACAAAAGgt.........................agGAATCAGACAGAATTATTTGT : 833652 269 : SerThrAsnIleLeuHisLysThrAspGlnThrLeuArgArgIleValSerGlnThrM : 288 |||.!!|||:!!||||||:!!.!!|||||||||||||||||||||||||||||||||| SerAlaAsnValLeuHisGlnAlaAspGlnThrLeuArgArgIleValSerGlnThrM 833653 : TCAGCTAACGTTCTGCATCAAGCTGACCAGACGCTCCGAAGGATTGTCTCTCAGACGA : 833712 289 : etLysGluAlaLysGly : 293 ||||||||||||||||| etLysGluAlaLysGly 833713 : TGAAGGAAGCAAAAGGT : 833729 vulgar: SPP00000052_2.0 0 293 . gi|472269467|gb|KB715222.1| 827354 833729 + 1258 M 72 216 5 0 2 I 0 2000 3 0 2 M 97 291 S 0 1 5 0 2 I 0 234 3 0 2 S 1 2 M 65 195 S 0 2 5 0 2 I 0 2755 3 0 2 S 1 1 M 25 75 5 0 2 I 0 491 3 0 2 M 32 96 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472269467|gb|KB715222.1| exonerate:protein2genome:local gene 827355 833729 1258 + . gene_id 1 ; sequence SPP00000052_2.0 ; gene_orientation + gi|472269467|gb|KB715222.1| exonerate:protein2genome:local cds 827355 827570 . + . gi|472269467|gb|KB715222.1| exonerate:protein2genome:local exon 827355 827570 . + . insertions 0 ; deletions 0 gi|472269467|gb|KB715222.1| exonerate:protein2genome:local splice5 827571 827572 . + . intron_id 1 ; splice_site "GT" gi|472269467|gb|KB715222.1| exonerate:protein2genome:local intron 827571 829574 . + . intron_id 1 gi|472269467|gb|KB715222.1| exonerate:protein2genome:local splice3 829573 829574 . + . intron_id 0 ; splice_site "AG" gi|472269467|gb|KB715222.1| exonerate:protein2genome:local cds 829575 829866 . + . gi|472269467|gb|KB715222.1| exonerate:protein2genome:local exon 829575 829866 . + . insertions 0 ; deletions 0 gi|472269467|gb|KB715222.1| exonerate:protein2genome:local splice5 829867 829868 . + . intron_id 2 ; splice_site "GT" gi|472269467|gb|KB715222.1| exonerate:protein2genome:local intron 829867 830104 . + . intron_id 2 gi|472269467|gb|KB715222.1| exonerate:protein2genome:local splice3 830103 830104 . + . intron_id 1 ; splice_site "AG" gi|472269467|gb|KB715222.1| exonerate:protein2genome:local cds 830105 830303 . + . gi|472269467|gb|KB715222.1| exonerate:protein2genome:local exon 830105 830303 . + . insertions 0 ; deletions 0 gi|472269467|gb|KB715222.1| exonerate:protein2genome:local splice5 830304 830305 . + . intron_id 3 ; splice_site "GT" gi|472269467|gb|KB715222.1| exonerate:protein2genome:local intron 830304 833062 . + . intron_id 3 gi|472269467|gb|KB715222.1| exonerate:protein2genome:local splice3 833061 833062 . + . intron_id 2 ; splice_site "AG" gi|472269467|gb|KB715222.1| exonerate:protein2genome:local cds 833063 833138 . + . gi|472269467|gb|KB715222.1| exonerate:protein2genome:local exon 833063 833138 . + . insertions 0 ; deletions 0 gi|472269467|gb|KB715222.1| exonerate:protein2genome:local splice5 833139 833140 . + . intron_id 4 ; splice_site "GT" gi|472269467|gb|KB715222.1| exonerate:protein2genome:local intron 833139 833633 . + . intron_id 4 gi|472269467|gb|KB715222.1| exonerate:protein2genome:local splice3 833632 833633 . + . intron_id 3 ; splice_site "AG" gi|472269467|gb|KB715222.1| exonerate:protein2genome:local cds 833634 833729 . + . gi|472269467|gb|KB715222.1| exonerate:protein2genome:local exon 833634 833729 . + . insertions 0 ; deletions 0 gi|472269467|gb|KB715222.1| exonerate:protein2genome:local similarity 827355 833729 1258 + . alignment_id 1 ; Query SPP00000052_2.0 ; Align 827355 1 216 ; Align 829575 73 291 ; Align 830107 171 195 ; Align 833064 237 75 ; Align 833634 262 96 # --- END OF GFF DUMP --- # -- completed exonerate analysis