TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000052_2.0 # Protein # Phosphoseryl-tRNA kinase (PSTK) # Human (Homo sapiens) (348 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472269467|gb|KB715222.1| Leptonychotes weddellii isolate WS11... 186 1e-45 gi|472269965|gb|KB714724.1| Leptonychotes weddellii isolate WS11... 37 2.4 gi|472267279|gb|KB717378.1| Leptonychotes weddellii isolate WS11... 35 9.3 gi|472269833|gb|KB714856.1| Leptonychotes weddellii isolate WS11... 35 9.3 >gi|472269467|gb|KB715222.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00516, whole genome shotgun sequence Length = 1136313 Score = 186 bits (473), Expect = 1e-45 Identities = 89/97 (91%), Positives = 92/97 (94%) Frame = +3 Query: 73 PSQWKLLRQELLKYLEYFLMAVINGCQMSVPPNRTEAMWEDFITCLKDQDLIFSAAFEAQ 132 PSQWKLLRQELLKYLEYFLMAVINGCQMSVPPNRT A+WEDFI+CLKDQDLI SAA E Q Sbjct: 829575 PSQWKLLRQELLKYLEYFLMAVINGCQMSVPPNRTAALWEDFISCLKDQDLISSAALETQ 829754 Query: 133 SCYLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARK 169 SCYLLTK AVSRPLFL+LDDNFYYQSMRYEVYQLARK Sbjct: 829755 SCYLLTKIAVSRPLFLILDDNFYYQSMRYEVYQLARK 829865 Score = 116 bits (290), Expect = 2e-24 Identities = 55/74 (74%), Positives = 63/74 (85%) Frame = +3 Query: 1 MKTAENIRGTGSDGPRKRGLCVLCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVMP 60 MKTAE+ RG S+GPR+ GLC+LCGLPAAGKST ARAL+HRL+QE+GWA+GVV YDDVMP Sbjct: 827355 MKTAEDARGARSEGPREIGLCLLCGLPAAGKSTLARALSHRLRQERGWAVGVVTYDDVMP 827534 Query: 61 DAFLAGARARPAPS 74 DAFL A ARP S Sbjct: 827535 DAFLEEASARPLVS 827576 Score = 115 bits (289), Expect = 3e-24 Identities = 54/65 (83%), Positives = 56/65 (86%) Frame = +1 Query: 171 SLGFCQLFLDCPLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPA 230 SLGFCQLFLDC LETCLQRNGQR Q LP ETIHLM RK+EKPNP KNAWEHNSLTI S Sbjct: 830107 SLGFCQLFLDCSLETCLQRNGQRCQPLPAETIHLMARKIEKPNPGKNAWEHNSLTIQSTP 830286 Query: 231 CASEA 235 C+SEA Sbjct: 830287 CSSEA 830301 Score = 58.2 bits (139), Expect = 8e-07 Identities = 26/32 (81%), Positives = 30/32 (93%) Frame = +3 Query: 262 DTDRIICSTNILHKTDQTLRRIVSQTMKEAKG 293 ++DRIICS N+LH+ DQTLRRIVSQTMKEAKG Sbjct: 833634 ESDRIICSANVLHQADQTLRRIVSQTMKEAKG 833729 Score = 47.8 bits (112), Expect = 0.001 Identities = 22/26 (84%), Positives = 25/26 (96%) Frame = +3 Query: 236 SLEVTDLLLTALENPVKYAEDNMEQK 261 SL++TDLLLTALENPVKY EDN+EQK Sbjct: 833061 SLKLTDLLLTALENPVKYIEDNVEQK 833138 >gi|472269965|gb|KB714724.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00018, whole genome shotgun sequence Length = 4741398 Score = 36.6 bits (83), Expect = 2.4 Identities = 21/57 (36%), Positives = 27/57 (47%) Frame = -3 Query: 181 CPLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPACASEASL 237 CP TC++ G P L P+ +H P NA + L IPS AC S+A L Sbjct: 721150 CPEPTCMRHIGADPDLLAPQRLHKAW-----PITWNNASMYEVLNIPSMACVSDACL 720995 >gi|472267279|gb|KB717378.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02672, whole genome shotgun sequence Length = 357217 Score = 34.7 bits (78), Expect = 9.3 Identities = 16/55 (29%), Positives = 24/55 (43%) Frame = -3 Query: 181 CPLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPACASEA 235 CP + GQ LPP+++H R+L P+P + E T+ C A Sbjct: 189803 CPCRLPTRPTGQHSACLPPQSVHAPARRLAAPSPAQALREA*GRTLDGETCPPHA 189639 >gi|472269833|gb|KB714856.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00150, whole genome shotgun sequence Length = 2437523 Score = 34.7 bits (78), Expect = 9.3 Identities = 20/51 (39%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Frame = -2 Query: 200 ETIHLMGRK-LEKPNPEKNAWEHNSLTIPSPACASEASLEVTDLLLTALEN 249 ET+HL+G + K NP++N HN P P E EV DLL+ + +N Sbjct: 50992 ETLHLVGSSHIRKGNPQRNPGIHNQRRRPRPLATLEK--EVKDLLILSNQN 50846 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.321 0.134 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,492,425,301 Number of Sequences: 16710 Number of extensions: 24518261 Number of successful extensions: 113320 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 993 Number of HSP's successfully gapped in prelim test: 831 Number of HSP's that attempted gapping in prelim test: 101265 Number of HSP's gapped (non-prelim): 33519 length of query: 348 length of database: 1,052,295,386 effective HSP length: 133 effective length of query: 215 effective length of database: 1,050,072,956 effective search space: 225765685540 effective search space used: 225765685540 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 78 (34.7 bits)