Command line: [exonerate -m p2g --showtargetgff -q ./Gpx6.fa -t Gpx6.carpeta/genomic.fa] Hostname: [ws116052] C4 Alignment: ------------ Query: SPP00000038_2.0 # Protein # Glutathione peroxidase 6 (GPx6) # Human (Homo sapiens) Target: gi|472269128|gb|KB715529.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00823, whole genome shotgun sequence Model: protein2genome:local Raw score: 780 Query range: 28 -> 220 Target range: 520219 -> 525075 29 : LysValAspCysAsnLysGlyValThrGlyThrIleTyrGluTyrGlyAlaLeuThrL : 48 :!!:!!|||||| !!|||! !|||! !||||||||||||!!:|||! !|||||||||| GlnMetAspCysTyrLysAspValLysGlyThrIleTyrAspTyrGluAlaLeuThrL 520220 : CAGATGGATTGCTACAAAGATGTGAAAGGCACAATCTATGACTATGAAGCCCTCACTC : 520277 49 : euAsnGlyGluGluTyrIleGlnPheLysGlnPheAlaGlyLysHisValLeuPheVa : 67 ||||||||:!!|||:!!||||||||||||!:!!:!|||||||||! !||||||||||| euAsnGlyLysGluHisIleGlnPheLysArgTyrAlaGlyLysProValLeuPheVa 520278 : TGAATGGAAAGGAGCACATTCAGTTCAAGCGGTATGCGGGCAAGCCCGTCCTCTTTGT : 520334 68 : lAsnValAlaAlaTyrUnkGlyLeuAlaAlaGlnTyrPro{G} >>>> Target I : 81 ||||||||||.!!||| ||||||.!!||||||||||||{|} 2797 lAsnValAlaThrTyrCysGlyLeuThrAlaGlnTyrPro{G}++ 520335 : CAACGTGGCCACCTACTGTGGCCTGACAGCTCAGTATCCT{G}gt............. : 520379 82 : ntron 1 >>>> {lu}LeuAsnAlaLeuGlnGluGluLeuLysAsnPheGlyValI : 95 bp {||}||||||||||||||||||||||||||| !!!.|||:!!: ++{lu}LeuAsnAlaLeuGlnGluGluLeuLysProLeuGlyLeuV 520380 : ............ag{AA}CTGAATGCACTTCAGGAGGAGCTGAAGCCCTTGGGCCTGG : 523215 96 : leValLeuAlaPheProCysAsnGlnPheGlyLysGlnGluProGlyThrAsnSerGl : 114 !!||||||!.!|||||||||||||||||||||||||||||||||||| !|||||||| alValLeuGlyPheProCysAsnGlnPheGlyLysGlnGluProGlyGluAsnSerGl 523216 : TTGTGTTGGGCTTTCCCTGCAACCAGTTCGGAAAGCAGGAACCAGGAGAGAACTCAGA : 523272 115 : uIleLeuLeuGlyLeu{Ly} >>>> Target Intron 2 >>>> {s}TyrVal : 122 |||||||! !||||||{||} 415 bp {|}|||||| uIleLeuProGlyLeu{Ly}++ ++{s}TyrVal 523273 : GATTCTTCCGGGACTG{AA}gt.........................ag{G}TATGTC : 523711 123 : CysProGlySerGlyPheValProSerPheGlnLeuPheGluLysGlyAspValAsnG : 142 !!||||||!!!|||!:!||||||!:!||||||||||||||||||||||||||||||| ArgProGlyArgGlyTyrValProAsnPheGlnLeuPheGluLysGlyAspValAsnG 523712 : CGTCCAGGGAGAGGATACGTACCCAACTTCCAGCTCTTTGAGAAAGGGGATGTGAATG : 523771 143 : lyGluLysGluGlnLysValPheThrPheLeuLys >>>> Target Intron 3 : 154 ||||||||||||||||||||||||||||||||||| 1068 bp lyGluLysGluGlnLysValPheThrPheLeuLys++ 523772 : GCGAGAAGGAACAGAAGGTCTTCACCTTCTTGAAGgt..................... : 523809 155 : >>>> AsnSerCysProProThrSerAspLeuLeuGlySerSerSerGlnLeuPheT : 171 !|||||||||! ! !!|||!!::!!||||||! !! !!!!!!.:!!! !| ++LeuSerCysProHisProSerGluValLeuGlyLeuPheArgHisIleSerT 523810 : ....agCTCTCCTGCCCTCACCCCTCTGAGGTTCTGGGCTTATTCAGACATATATCCT : 524926 172 : rpGluProMetLysValHisAspIleArgTrpAsnPheGluLysPheLeuValGlyPr : 190 ||!!:|||:!:||||||||||||||||||||||||||||||||||||||||||||||| rpAspProValLysValHisAspIleArgTrpAsnPheGluLysPheLeuValGlyPr 524927 : GGGATCCCGTAAAGGTCCACGACATCCGCTGGAACTTTGAGAAGTTCCTGGTGGGGCC : 524983 191 : oAspGlyValProValMetHisTrpPheHisGlnAlaProValSerThrValLysSer : 209 |||||||||||||||||||!.!|||||||||!:!.!!|||:!!||||||||||||||| oAspGlyValProValMetArgTrpPheHisArgThrProIleSerThrValLysSer 524984 : TGATGGAGTCCCTGTCATGCGCTGGTTCCACCGGACTCCCATCAGCACGGTCAAGTCA : 525040 210 : AspIleLeuGluTyrLeuLysGlnPheAsnThr : 220 |||||||||||||||||||||||||||!!.!:! AspIleLeuGluTyrLeuLysGlnPheLysSer 525041 : GACATCCTGGAGTACCTGAAACAGTTCAAAAGT : 525075 vulgar: SPP00000038_2.0 28 220 . gi|472269128|gb|KB715529.1| 520219 525075 + 780 M 52 156 S 0 1 5 0 2 I 0 2793 3 0 2 S 1 2 M 38 114 S 0 2 5 0 2 I 0 411 3 0 2 S 1 1 M 33 99 5 0 2 I 0 1064 3 0 2 M 67 201 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-15 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472269128|gb|KB715529.1| exonerate:protein2genome:local gene 520220 525075 780 + . gene_id 1 ; sequence SPP00000038_2.0 ; gene_orientation + gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 520220 520376 . + . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 520220 520376 . + . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 520377 520378 . + . intron_id 1 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 520377 523173 . + . intron_id 1 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 523172 523173 . + . intron_id 0 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 523174 523291 . + . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 523174 523291 . + . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 523292 523293 . + . intron_id 2 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 523292 523706 . + . intron_id 2 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 523705 523706 . + . intron_id 1 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 523707 523806 . + . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 523707 523806 . + . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 523807 523808 . + . intron_id 3 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 523807 524874 . + . intron_id 3 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 524873 524874 . + . intron_id 2 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 524875 525075 . + . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 524875 525075 . + . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local similarity 520220 525075 780 + . alignment_id 1 ; Query SPP00000038_2.0 ; Align 520220 29 156 ; Align 523176 82 114 ; Align 523708 121 99 ; Align 524875 154 201 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000038_2.0 # Protein # Glutathione peroxidase 6 (GPx6) # Human (Homo sapiens) Target: gi|472269128|gb|KB715529.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00823, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 902 Query range: 28 -> 220 Target range: 492262 -> 485954 29 : LysValAspCysAsnLysGlyValThrGlyThrIleTyrGluTyrGlyAlaLeuThrL : 48 :!!:!!|||||| !!||||||||||||||||||||||||||||||||||||||||||| GlnMetAspCysTyrLysGlyValThrGlyThrIleTyrGluTyrGlyAlaLeuThrL 492262 : CAGATGGATTGCTACAAAGGGGTGACGGGCACCATCTATGAGTACGGAGCCCTCACCC : 492205 49 : euAsnGlyGluGluTyrIleGlnPheLysGlnPheAlaGlyLysHisValLeuPheVa : 67 |||||! !|||||||||||||||||||||!:!!:!||||||||||||||||||||||| euAsnGluGluGluTyrIleGlnPheLysArgTyrAlaGlyLysHisValLeuPheVa 492204 : TCAATGAAGAGGAGTACATTCAGTTCAAGCGGTATGCGGGCAAGCACGTCCTCTTTGT : 492148 68 : lAsnValAlaAlaTyrUnkGlyLeuAlaAlaGlnTyrPro{G} >>>> Target I : 81 ||||||||||.!!||| ||||||.!!||||||||||||{|} 4302 lAsnValAlaThrTyr***GlyLeuThrAlaGlnTyrPro{G}++ 492147 : CAACGTGGCCACCTACTGAGGCCTGACAGCTCAGTATCCT{G}gt............. : 492103 82 : ntron 1 >>>> {lu}LeuAsnAlaLeuGlnGluGluLeuLysAsnPheGlyValI : 95 bp {||}|||||||||||||||!!:||||||||| !! !||||||: ++{lu}LeuAsnAlaLeuGlnAspGluLeuLysProSerGlyValV 492102 : ............ag{AA}CTGAATGCATTGCAGGACGAGCTGAAGCCTTCTGGTGTCG : 487762 96 : leValLeuAlaPheProCysAsnGlnPheGlyLysGlnGluProGlyThrAsnSerGl : 114 !!||||||!.!||||||||||||||||||||||||||||||||||||! !|||||||| alValLeuGlyPheProCysAsnGlnPheGlyLysGlnGluProGlyLysAsnSerGl 487761 : TCGTGCTGGGCTTTCCCTGCAACCAGTTTGGAAAACAAGAACCAGGGAAGAACTCAGA : 487705 115 : uIleLeuLeuGlyLeu{Ly} >>>> Target Intron 2 >>>> {s}TyrVal : 122 |||||||! !||||||{||} 474 bp {|}|||||| uIleLeuSerGlyLeu{Ly}++ ++{s}TyrVal 487704 : GATCCTTTCTGGGCTC{AA}gt.........................ag{G}TATGTG : 487207 123 : CysProGlySerGlyPheValProSerPheGlnLeuPheGluLysGlyAspValAsnG : 142 !!||||||.!!||||||||||||!:!||||||||||||||||||||||||||||||| ArgProGlyGlyGlyPheValProAsnPheGlnLeuPheGluLysGlyAspValAsnG 487206 : CGTCCAGGTGGTGGCTTTGTCCCCAATTTTCAGCTCTTTGAGAAAGGGGATGTGAATG : 487147 143 : lyGluLysGluGlnLysValPheThrPheLeuLys >>>> Target Intron 3 : 154 ||||||||||||||||||||||||||||||||||| 956 bp lyGluLysGluGlnLysValPheThrPheLeuLys++ 487146 : GAGAAAAAGAACAGAAGGTCTTTACCTTCCTGAAGgt..................... : 487109 155 : >>>> AsnSerCysProProThrSerAspLeuLeuGlySerSerSerGlnLeuPheT : 171 ||||||||||||||||||||||||||||||||||||:!!||||||||||||| ++AsnSerCysProProThrSerAspLeuLeuGlySerAlaSerGlnLeuPheT 487108 : ....agAACTCCTGCCCTCCAACCTCTGATCTTTTGGGCTCAGCAAGCCAACTCTTCT : 486104 172 : rpGluProMetLysValHisAspIleArgTrpAsnPheGluLysPheLeuValGlyPr : 190 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| rpGluProMetLysValHisAspIleArgTrpAsnPheGluLysPheLeuValGlyPr 486103 : GGGAGCCCATGAAGGTCCACGACATCCGCTGGAACTTTGAGAAGTTCCTGGTGGGGCC : 486047 191 : oAspGlyValProValMetHisTrpPheHisGlnAlaProValSerThrValLysSer : 209 |||||||||||||||||||!.!|||||||||:!!|||||||||||||||||||||||| oAspGlyValProValMetArgTrpPheHisLysAlaProValSerThrValLysSer 486046 : TGATGGAGTCCCTGTCATGCGCTGGTTCCACAAGGCTCCCGTCAGCACGGTCAAGTCA : 485990 210 : AspIleLeuGluTyrLeuLysGlnPheAsnThr : 220 |||||||||||||||||||||||||||!!.||| AspIleLeuGluTyrLeuLysGlnPheLysThr 485989 : GACATCTTGGAGTACCTGAAACAGTTCAAAACC : 485955 vulgar: SPP00000038_2.0 28 220 . gi|472269128|gb|KB715529.1| 492262 485954 - 902 M 52 156 S 0 1 5 0 2 I 0 4298 3 0 2 S 1 2 M 38 114 S 0 2 5 0 2 I 0 470 3 0 2 S 1 1 M 33 99 5 0 2 I 0 952 3 0 2 M 67 201 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-15 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472269128|gb|KB715529.1| exonerate:protein2genome:local gene 485955 492262 902 - . gene_id 1 ; sequence SPP00000038_2.0 ; gene_orientation + gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 492106 492262 . - . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 492106 492262 . - . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 492104 492105 . - . intron_id 1 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 487804 492105 . - . intron_id 1 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 487804 487805 . - . intron_id 0 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 487686 487803 . - . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 487686 487803 . - . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 487684 487685 . - . intron_id 2 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 487212 487685 . - . intron_id 2 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 487212 487213 . - . intron_id 1 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 487112 487211 . - . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 487112 487211 . - . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 487110 487111 . - . intron_id 3 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 486156 487111 . - . intron_id 3 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 486156 486157 . - . intron_id 2 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 485955 486155 . - . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 485955 486155 . - . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local similarity 485955 492262 902 - . alignment_id 1 ; Query SPP00000038_2.0 ; Align 492263 29 156 ; Align 487802 82 114 ; Align 487211 121 99 ; Align 486156 154 201 # --- END OF GFF DUMP --- # -- completed exonerate analysis