Command line: [exonerate -m p2g --showtargetgff -q ./Gpx5.fa -t Gpx5.carpeta/genomic.fa] Hostname: [ws116052] C4 Alignment: ------------ Query: SPP00000036_2.0 # Protein # Glutathione peroxidase 5 (GPx5) # Human (Homo sapiens) Target: gi|472269128|gb|KB715529.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00823, whole genome shotgun sequence Model: protein2genome:local Raw score: 953 Query range: 0 -> 221 Target range: 517325 -> 525078 1 : MetThrThrGlnLeuArgValValHisLeuLeuProLeuLeuLeuAlaCysPheValG : 20 ||||||.!!|||||||||!.! !:!!|||:!!||||||||||||!.! !!|||..!| MetThrAlaGlnLeuArgAlaSerTyrLeuValProLeuLeuLeuValSerPheThrG 517326 : ATGACTGCACAGTTAAGGGCCTCTTACCTTGTTCCTCTTCTGCTAGTCAGCTTTACAC : 517383 21 : lnThrSerProLysGlnGluLysMetLys >>>> Target Intron 1 >>>> : 30 |||||!:! !!|||! |||||||||||| 2810 bp lnThrAsnAlaLysProGluLysMetLys++ ++ 517384 : AGACAAATGCCAAGCCTGAGAAGATGAAGgt.........................ag : 520221 31 : MetAspCysHisLysAspGluLysGlyThrIleTyrAspTyrGluAlaIleAlaLeuA : 49 |||||||||:!!||||||! !|||||||||||||||||||||||||||:!!.!!|||| MetAspCysTyrLysAspValLysGlyThrIleTyrAspTyrGluAlaLeuThrLeuA 520222 : ATGGATTGCTACAAAGATGTGAAAGGCACAATCTATGACTATGAAGCCCTCACTCTGA : 520280 50 : snLysAsnGluTyrValSerPheLysGlnTyrValGlyLysHisIleLeuPheValAs : 68 || !!!.|||:!!:!!..!||||||!:!|||!.!||||||! !:!!||||||||||| snGlyLysGluHisIleGlnPheLysArgTyrAlaGlyLysProValLeuPheValAs 520281 : ATGGAAAGGAGCACATTCAGTTCAAGCGGTATGCGGGCAAGCCCGTCCTCTTTGTCAA : 520337 69 : nValAlaThrTyrCysGlyLeuThrAlaGlnTyrPro{G} >>>> Target Intr : 81 |||||||||||||||||||||||||||||||||||||{|} 2797 bp nValAlaThrTyrCysGlyLeuThrAlaGlnTyrPro{G}++ 520338 : CGTGGCCACCTACTGTGGCCTGACAGCTCAGTATCCT{G}gt................ : 520379 82 : on 2 >>>> {lu}LeuAsnAlaLeuGlnGluGluLeuLysProTyrGlyLeuValV : 96 {||}||||||||||||||||||||||||||||||! |||||||||| ++{lu}LeuAsnAlaLeuGlnGluGluLeuLysProLeuGlyLeuValV 520380 : .........ag{AA}CTGAATGCACTTCAGGAGGAGCTGAAGCCCTTGGGCCTGGTTG : 523218 97 : alLeuGlyPheProCysAsnGlnPheGlyLysGlnGluProGlyAspAsnLysGluIl : 115 ||||||||||||||||||||||||||||||||||||||||||||!!:|||..!||||| alLeuGlyPheProCysAsnGlnPheGlyLysGlnGluProGlyGluAsnSerGluIl 523219 : TGTTGGGCTTTCCCTGCAACCAGTTCGGAAAGCAGGAACCAGGAGAGAACTCAGAGAT : 523275 116 : eLeuProGlyLeu{Ly} >>>> Target Intron 3 >>>> {s}TyrValArg : 123 |||||||||||||{||} 415 bp {|}||||||||| eLeuProGlyLeu{Ly}++ ++{s}TyrValArg 523276 : TCTTCCGGGACTG{AA}gt.........................ag{G}TATGTCCGT : 523714 124 : ProGlyGlyGlyPheValProSerPheGlnLeuPheGluLysGlyAspValAsnGlyG : 143 |||||| !!|||!:!||||||!:!|||||||||||||||||||||||||||||||||| ProGlyArgGlyTyrValProAsnPheGlnLeuPheGluLysGlyAspValAsnGlyG 523715 : CCAGGGAGAGGATACGTACCCAACTTCCAGCTCTTTGAGAAAGGGGATGTGAATGGCG : 523774 144 : luLysGluGlnLysValPheSerPheLeuLys >>>> Target Intron 4 >>> : 154 ||||||||||||||||||||!!!||||||||| 1068 bp luLysGluGlnLysValPheThrPheLeuLys++ 523775 : AGAAGGAACAGAAGGTCTTCACCTTCTTGAAGgt........................ : 523809 155 : > HisSerCysProHisProSerGluIleLeuGlyThrPheLysSerIleSerTrpA : 172 ! !|||||||||||||||||||||:!!|||||| !|||!:! !|||||||||| ++LeuSerCysProHisProSerGluValLeuGlyLeuPheArgHisIleSerTrpA 523810 : .agCTCTCCTGCCCTCACCCCTCTGAGGTTCTGGGCTTATTCAGACATATATCCTGGG : 524929 173 : spProValLysValHisAspIleArgTrpAsnPheGluLysPheLeuValGlyProAs : 191 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| spProValLysValHisAspIleArgTrpAsnPheGluLysPheLeuValGlyProAs 524930 : ATCCCGTAAAGGTCCACGACATCCGCTGGAACTTTGAGAAGTTCCTGGTGGGGCCTGA : 524986 192 : pGlyIleProValMetArgTrpSerHisArgAlaThrValSerSerValLysThrAsp : 210 ||||:!!|||||||||||||||! !||||||.!! !!:!!|||!!!||||||:!!||| pGlyValProValMetArgTrpPheHisArgThrProIleSerThrValLysSerAsp 524987 : TGGAGTCCCTGTCATGCGCTGGTTCCACCGGACTCCCATCAGCACGGTCAAGTCAGAC : 525043 211 : IleLeuAlaTyrLeuLysGlnPheLysThrLys : 221 ||||||! !||||||||||||||||||!:!||| IleLeuGluTyrLeuLysGlnPheLysSerLys 525044 : ATCCTGGAGTACCTGAAACAGTTCAAAAGTAAA : 525078 vulgar: SPP00000036_2.0 0 221 . gi|472269128|gb|KB715529.1| 517325 525078 + 953 M 29 87 5 0 2 I 0 2806 3 0 2 M 51 153 S 0 1 5 0 2 I 0 2793 3 0 2 S 1 2 M 38 114 S 0 2 5 0 2 I 0 411 3 0 2 S 1 1 M 33 99 5 0 2 I 0 1064 3 0 2 M 68 204 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-15 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472269128|gb|KB715529.1| exonerate:protein2genome:local gene 517326 525078 953 + . gene_id 1 ; sequence SPP00000036_2.0 ; gene_orientation + gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 517326 517412 . + . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 517326 517412 . + . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 517413 517414 . + . intron_id 1 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 517413 520222 . + . intron_id 1 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 520221 520222 . + . intron_id 0 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 520223 520376 . + . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 520223 520376 . + . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 520377 520378 . + . intron_id 2 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 520377 523173 . + . intron_id 2 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 523172 523173 . + . intron_id 1 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 523174 523291 . + . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 523174 523291 . + . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 523292 523293 . + . intron_id 3 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 523292 523706 . + . intron_id 3 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 523705 523706 . + . intron_id 2 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 523707 523806 . + . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 523707 523806 . + . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 523807 523808 . + . intron_id 4 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 523807 524874 . + . intron_id 4 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 524873 524874 . + . intron_id 3 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 524875 525078 . + . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 524875 525078 . + . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local similarity 517326 525078 953 + . alignment_id 1 ; Query SPP00000036_2.0 ; Align 517326 1 87 ; Align 520223 30 153 ; Align 523176 82 114 ; Align 523708 121 99 ; Align 524875 154 204 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000036_2.0 # Protein # Glutathione peroxidase 5 (GPx5) # Human (Homo sapiens) Target: gi|472269128|gb|KB715529.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00823, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 818 Query range: 27 -> 221 Target range: 492265 -> 485951 28 : MetLysMetAspCysHisLysAspGluLysGlyThrIleTyrAspTyrGluAlaIleA : 47 .!.:!!|||||||||:!!|||! ! !! !||||||||||||!!:|||! !|||:!!. PheGlnMetAspCysTyrLysGlyValThrGlyThrIleTyrGluTyrGlyAlaLeuT 492265 : TTCCAGATGGATTGCTACAAAGGGGTGACGGGCACCATCTATGAGTACGGAGCCCTCA : 492208 48 : laLeuAsnLysAsnGluTyrValSerPheLysGlnTyrValGlyLysHisIleLeuPh : 66 !!||||||:!!.!.||||||:!!..!||||||!:!|||!.!|||||||||:!!||||| hrLeuAsnGluGluGluTyrIleGlnPheLysArgTyrAlaGlyLysHisValLeuPh 492207 : CCCTCAATGAAGAGGAGTACATTCAGTTCAAGCGGTATGCGGGCAAGCACGTCCTCTT : 492151 67 : eValAsnValAlaThrTyrCysGlyLeuThrAlaGlnTyrPro{G} >>>> Targe : 81 |||||||||||||||||||!! |||||||||||||||||||||{|} 4 eValAsnValAlaThrTyr***GlyLeuThrAlaGlnTyrPro{G}++ 492150 : TGTCAACGTGGCCACCTACTGAGGCCTGACAGCTCAGTATCCT{G}gt.......... : 492103 82 : t Intron 1 >>>> {lu}LeuAsnAlaLeuGlnGluGluLeuLysProTyrGlyL : 94 302 bp {||}|||||||||||||||!!:||||||||||||! !|||: ++{lu}LeuAsnAlaLeuGlnAspGluLeuLysProSerGlyV 492102 : ...............ag{AA}CTGAATGCATTGCAGGACGAGCTGAAGCCTTCTGGTG : 487765 95 : euValValLeuGlyPheProCysAsnGlnPheGlyLysGlnGluProGlyAspAsnLy : 113 !!|||||||||||||||||||||||||||||||||||||||||||||||| ! |||.. alValValLeuGlyPheProCysAsnGlnPheGlyLysGlnGluProGlyLysAsnSe 487764 : TCGTCGTGCTGGGCTTTCCCTGCAACCAGTTTGGAAAACAAGAACCAGGGAAGAACTC : 487708 114 : sGluIleLeuProGlyLeu{Ly} >>>> Target Intron 2 >>>> {s}Tyr : 121 !||||||||| !!||||||{||} 474 bp {|}||| rGluIleLeuSerGlyLeu{Ly}++ ++{s}Tyr 487707 : AGAGATCCTTTCTGGGCTC{AA}gt.........................ag{G}TAT : 487210 122 : ValArgProGlyGlyGlyPheValProSerPheGlnLeuPheGluLysGlyAspValA : 141 |||||||||||||||||||||||||||!:!|||||||||||||||||||||||||||| ValArgProGlyGlyGlyPheValProAsnPheGlnLeuPheGluLysGlyAspValA 487209 : GTGCGTCCAGGTGGTGGCTTTGTCCCCAATTTTCAGCTCTTTGAGAAAGGGGATGTGA : 487150 142 : snGlyGluLysGluGlnLysValPheSerPheLeuLys >>>> Target Intron : 154 ||||||||||||||||||||||||||!!!||||||||| 956 bp snGlyGluLysGluGlnLysValPheThrPheLeuLys++ 487149 : ATGGAGAAAAAGAACAGAAGGTCTTTACCTTCCTGAAGgt.................. : 487109 155 : 3 >>>> HisSerCysProHisProSerGluIleLeuGlyThrPheLysSerIleS : 170 :!!|||||||||! !!|||!!::!!||||||:!! !....!:!!! ++AsnSerCysProProThrSerAspLeuLeuGlySerAlaSerGlnLeuP 487108 : .......agAACTCCTGCCCTCCAACCTCTGATCTTTTGGGCTCAGCAAGCCAACTCT : 486107 171 : erTrpAspProValLysValHisAspIleArgTrpAsnPheGluLysPheLeuValGl : 189 !|||!!:|||:!!|||||||||||||||||||||||||||||||||||||||||||| heTrpGluProMetLysValHisAspIleArgTrpAsnPheGluLysPheLeuValGl 486106 : TCTGGGAGCCCATGAAGGTCCACGACATCCGCTGGAACTTTGAGAAGTTCCTGGTGGG : 486050 190 : yProAspGlyIleProValMetArgTrpSerHisArgAlaThrValSerSerValLys : 208 ||||||||||:!!|||||||||||||||! !|||!:!||| !!||||||!!!|||||| yProAspGlyValProValMetArgTrpPheHisLysAlaProValSerThrValLys 486049 : GCCTGATGGAGTCCCTGTCATGCGCTGGTTCCACAAGGCTCCCGTCAGCACGGTCAAG : 485993 209 : ThrAspIleLeuAlaTyrLeuLysGlnPheLysThrLys : 221 :!!|||||||||! !|||||||||||||||||||||:!! SerAspIleLeuGluTyrLeuLysGlnPheLysThrGlu 485992 : TCAGACATCTTGGAGTACCTGAAACAGTTCAAAACCGAG : 485952 vulgar: SPP00000036_2.0 27 221 . gi|472269128|gb|KB715529.1| 492265 485951 - 818 M 53 159 S 0 1 5 0 2 I 0 4298 3 0 2 S 1 2 M 38 114 S 0 2 5 0 2 I 0 470 3 0 2 S 1 1 M 33 99 5 0 2 I 0 952 3 0 2 M 68 204 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-15 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472269128|gb|KB715529.1| exonerate:protein2genome:local gene 485952 492265 818 - . gene_id 1 ; sequence SPP00000036_2.0 ; gene_orientation + gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 492106 492265 . - . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 492106 492265 . - . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 492104 492105 . - . intron_id 1 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 487804 492105 . - . intron_id 1 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 487804 487805 . - . intron_id 0 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 487686 487803 . - . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 487686 487803 . - . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 487684 487685 . - . intron_id 2 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 487212 487685 . - . intron_id 2 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 487212 487213 . - . intron_id 1 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 487112 487211 . - . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 487112 487211 . - . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice5 487110 487111 . - . intron_id 3 ; splice_site "GT" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local intron 486156 487111 . - . intron_id 3 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local splice3 486156 486157 . - . intron_id 2 ; splice_site "AG" gi|472269128|gb|KB715529.1| exonerate:protein2genome:local cds 485952 486155 . - . gi|472269128|gb|KB715529.1| exonerate:protein2genome:local exon 485952 486155 . - . insertions 0 ; deletions 0 gi|472269128|gb|KB715529.1| exonerate:protein2genome:local similarity 485952 492265 818 - . alignment_id 1 ; Query SPP00000036_2.0 ; Align 492266 28 159 ; Align 487802 82 114 ; Align 487211 121 99 ; Align 486156 154 204 # --- END OF GFF DUMP --- # -- completed exonerate analysis