Command line: [exonerate -m p2g --showtargetgff -q ./Gpx4.fa -t Gpx4.carpeta/genomic.fa] Hostname: [ws116052] C4 Alignment: ------------ Query: SPP00000026_2.0 # Protein # Glutathione peroxidase 4 (GPx4) # Human (Homo sapiens) Target: gi|472268710|gb|KB715947.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01241, whole genome shotgun sequence Model: protein2genome:local Raw score: 961 Query range: 0 -> 196 Target range: 570735 -> 572906 1 : MetSerLeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeuA : 20 |||!:!|||.!!||||||! !||||||||||||||||||||||||||||||||||||| MetAsnLeuSerArgLeuTyrArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeuA 570736 : ATGAACCTCAGCCGCCTGTACCGCTTGCTGAAGCCTGCGCTGCTGTGCGGGGCCCTGG : 570793 21 : laAlaProGlyLeuAlaGlyThrMet >>>> Target Intron 1 >>>> Cys : 29 |||||||||||||||||.!!|||||| 944 bp ||| laAlaProGlyLeuAlaSerThrMet++ ++Cys 570794 : CCGCGCCGGGTCTGGCCAGCACCATGgt.........................agTGC : 571764 30 : AlaSerArgAspAspTrpArgCysAlaArgSerMetHisGluPheSerAlaLysAspI : 49 |||||||||||||||||||||||||||!:!|||||||||||||||||||||||||||| AlaSerArgAspAspTrpArgCysAlaGlnSerMetHisGluPheSerAlaLysAspI 571765 : GCGTCCCGAGACGACTGGCGCTGTGCTCAGTCTATGCACGAGTTCTCCGCCAAGGACA : 571824 50 : leAspGlyHisMetValAsnLeuAspLysTyr{Ar} >>>> Target Intron 2 : 60 |||||||| !|||||||||||||||||||||{||} 83 bp leAspGlySerMetValAsnLeuAspLysTyr{Ar}++ 571825 : TCGATGGAAGCATGGTTAACCTGGACAAGTAC{CG}gt.................... : 571861 61 : >>>> {g}GlyPheValCysIleValThrAsnValAlaSerGlnUnkGlyLysThr : 76 {|}|||||||||||||||||||||||||||||||||||| ||||||||| ++{g}GlyPheValCysIleValThrAsnValAlaSerGln***GlyLysThr 571862 : .....ag{G}GGCTTCGTGTGCATCGTCACCAACGTGGCCTCGCAATGAGGCAAAACA : 571988 77 : GluValAsnTyrThrGlnLeuValAspLeuHisAlaArgTyrAlaGluCysGlyLeuA : 96 |||||||||||||||||||||||||||||||||||||||!:!|||||||||||||||| GluValAsnTyrThrGlnLeuValAspLeuHisAlaArgPheAlaGluCysGlyLeuA 571989 : GAAGTAAACTACACTCAGCTTGTCGACCTGCACGCCCGATTCGCTGAGTGCGGTTTAC : 572048 97 : rgIleLeuAlaPheProCysAsnGlnPheGlyLysGln >>>> Target Intron : 109 ||||||||||||||||||||||||||||||||!:!||| 114 bp rgIleLeuAlaPheProCysAsnGlnPheGlyArgGln++ 572049 : GGATCCTGGCCTTCCCTTGCAACCAGTTCGGGAGGCAGgt.................. : 572089 110 : 3 >>>> GluProGlySerAsnGluGluIleLysGluPheAlaAlaGlyTyrAsnV : 125 |||||||||||||||! !||||||||||||||||||||||||||||||| ++GluProGlySerAsnAlaGluIleLysGluPheAlaAlaGlyTyrAsnV 572090 : .......agGAGCCAGGGAGTAATGCGGAGATCAAAGAGTTCGCTGCTGGCTATAATG : 572249 126 : alLysPheAspMetPheSerLysIleCysValAsnGlyAspAspAlaHisProLeuTr : 144 ||||||||||||||!:!||||||||||||||||||||||||||||||||||||||||| alLysPheAspMetTyrSerLysIleCysValAsnGlyAspAspAlaHisProLeuTr 572250 : TCAAATTCGATATGTACAGCAAGATCTGTGTGAACGGAGACGATGCTCACCCGCTGTG : 572306 145 : pLysTrpMetLysIleGlnProLysGlyLysGlyIleLeuGly{As} >>>> Targ : 159 |||||||||||||:!!||||||||||||!:!||||||||||||{||} pLysTrpMetLysValGlnProLysGlyArgGlyIleLeuGly{As}++ 572307 : GAAGTGGATGAAAGTCCAGCCCAAGGGCAGGGGCATCCTGGGA{AA}gt......... : 572355 160 : et Intron 4 >>>> {n}AlaIleLysTrpAsnPheThrLys >>>> Target : 168 117 bp {|}|||||||||||||||||||||||| 2 ++{n}AlaIleLysTrpAsnPheThrLys++ 572356 : ................ag{T}GCCATCAAATGGAATTTCACCAAGgt........... : 572497 169 : Intron 5 >>>> PheLeuIleAspLysAsnGlyCysValValLysArgTyrGly : 181 46 bp |||||||||||||||||||||||||||||||||||||||||| ++PheLeuIleAspLysAsnGlyCysValValLysArgTyrGly 572498 : ..............agTTCCTCATTGACAAGAACGGCTGTGTGGTGAAGCGTTATGGT : 572780 182 : ProMetGluGluProLeu >>>> Target Intron 6 >>>> ValIleGluLy : 191 |||||||||||||||||| 79 bp ||||||||||| ProMetGluGluProLeu++ ++ValIleGluLy 572781 : CCCATGGAAGAGCCCCTGgt.........................agGTAATAGAGAA : 572889 192 : sAspLeuProHisTyr : 196 |||||||||| !||| sAspLeuProCysTyr 572890 : GGACTTGCCCTGCTAC : 572906 vulgar: SPP00000026_2.0 0 196 . gi|472268710|gb|KB715947.1| 570735 572906 + 961 M 28 84 5 0 2 I 0 940 3 0 2 M 31 93 S 0 2 5 0 2 I 0 79 3 0 2 S 1 1 M 48 144 5 0 2 I 0 110 3 0 2 M 50 150 S 0 2 5 0 2 I 0 113 3 0 2 S 1 1 M 8 24 5 0 2 I 0 242 3 0 2 M 20 60 5 0 2 I 0 75 3 0 2 M 9 27 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2013-11-15 ##type DNA # # # seqname source feature start end score strand frame attributes # gi|472268710|gb|KB715947.1| exonerate:protein2genome:local gene 570736 572906 961 + . gene_id 1 ; sequence SPP00000026_2.0 ; gene_orientation + gi|472268710|gb|KB715947.1| exonerate:protein2genome:local cds 570736 570819 . + . gi|472268710|gb|KB715947.1| exonerate:protein2genome:local exon 570736 570819 . + . insertions 0 ; deletions 0 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice5 570820 570821 . + . intron_id 1 ; splice_site "GT" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local intron 570820 571763 . + . intron_id 1 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice3 571762 571763 . + . intron_id 0 ; splice_site "AG" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local cds 571764 571858 . + . gi|472268710|gb|KB715947.1| exonerate:protein2genome:local exon 571764 571858 . + . insertions 0 ; deletions 0 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice5 571859 571860 . + . intron_id 2 ; splice_site "GT" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local intron 571859 571941 . + . intron_id 2 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice3 571940 571941 . + . intron_id 1 ; splice_site "AG" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local cds 571942 572086 . + . gi|472268710|gb|KB715947.1| exonerate:protein2genome:local exon 571942 572086 . + . insertions 0 ; deletions 0 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice5 572087 572088 . + . intron_id 3 ; splice_site "GT" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local intron 572087 572200 . + . intron_id 3 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice3 572199 572200 . + . intron_id 2 ; splice_site "AG" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local cds 572201 572352 . + . gi|472268710|gb|KB715947.1| exonerate:protein2genome:local exon 572201 572352 . + . insertions 0 ; deletions 0 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice5 572353 572354 . + . intron_id 4 ; splice_site "GT" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local intron 572353 572469 . + . intron_id 4 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice3 572468 572469 . + . intron_id 3 ; splice_site "AG" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local cds 572470 572494 . + . gi|472268710|gb|KB715947.1| exonerate:protein2genome:local exon 572470 572494 . + . insertions 0 ; deletions 0 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice5 572495 572496 . + . intron_id 5 ; splice_site "GT" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local intron 572495 572740 . + . intron_id 5 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice3 572739 572740 . + . intron_id 4 ; splice_site "AG" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local cds 572741 572800 . + . gi|472268710|gb|KB715947.1| exonerate:protein2genome:local exon 572741 572800 . + . insertions 0 ; deletions 0 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice5 572801 572802 . + . intron_id 6 ; splice_site "GT" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local intron 572801 572879 . + . intron_id 6 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local splice3 572878 572879 . + . intron_id 5 ; splice_site "AG" gi|472268710|gb|KB715947.1| exonerate:protein2genome:local cds 572880 572906 . + . gi|472268710|gb|KB715947.1| exonerate:protein2genome:local exon 572880 572906 . + . insertions 0 ; deletions 0 gi|472268710|gb|KB715947.1| exonerate:protein2genome:local similarity 570736 572906 961 + . alignment_id 1 ; Query SPP00000026_2.0 ; Align 570736 1 84 ; Align 571764 29 93 ; Align 571943 61 144 ; Align 572201 109 150 ; Align 572471 160 24 ; Align 572741 168 60 ; Align 572880 188 27 # --- END OF GFF DUMP --- # -- completed exonerate analysis