TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000026_2.0 # Protein # Glutathione peroxidase 4 (GPx4) #
Human (Homo sapiens)
(197 letters)
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno
me.fa
16,710 sequences; 3,156,886,159 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|472268710|gb|KB715947.1| Leptonychotes weddellii isolate WS11... 183 2e-59
gi|472269405|gb|KB715284.1| Leptonychotes weddellii isolate WS11... 132 8e-30
gi|472268596|gb|KB716061.1| Leptonychotes weddellii isolate WS11... 95 9e-28
gi|472269737|gb|KB714952.1| Leptonychotes weddellii isolate WS11... 81 3e-14
gi|472269606|gb|KB715083.1| Leptonychotes weddellii isolate WS11... 70 5e-11
gi|472268977|gb|KB715680.1| Leptonychotes weddellii isolate WS11... 61 4e-08
gi|472264638|gb|KB720019.1| Leptonychotes weddellii isolate WS11... 60 9e-08
gi|472268783|gb|KB715874.1| Leptonychotes weddellii isolate WS11... 36 2e-05
gi|472269128|gb|KB715529.1| Leptonychotes weddellii isolate WS11... 46 0.001
gi|472269970|gb|KB714719.1| Leptonychotes weddellii isolate WS11... 45 0.003
gi|472268639|gb|KB716018.1| Leptonychotes weddellii isolate WS11... 44 0.004
gi|472269781|gb|KB714908.1| Leptonychotes weddellii isolate WS11... 41 0.033
gi|472268341|gb|KB716316.1| Leptonychotes weddellii isolate WS11... 33 6.9
>gi|472268710|gb|KB715947.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold01241, whole genome
shotgun sequence
Length = 781753
Score = 183 bits (465), Expect(2) = 2e-59
Identities = 92/138 (66%), Positives = 98/138 (71%), Gaps = 38/138 (27%)
Frame = +2
Query: 59 YRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQ---------- 108
+RGFVCIVTNVASQ GKTEVNYTQLVDLHAR+AECGLRILAFPCNQFG+Q
Sbjct: 571937 FRGFVCIVTNVASQ*GKTEVNYTQLVDLHARFAECGLRILAFPCNQFGRQVRGWLCPWLC 572116
Query: 109 ----------------------------EPGSNEEIKEFAAGYNVKFDMFSKICVNGDDA 140
EPGSN EIKEFAAGYNVKFDM+SKICVNGDDA
Sbjct: 572117 PRVSRCGERGPTGAQAPPHSPPHPCLPQEPGSNAEIKEFAAGYNVKFDMYSKICVNGDDA 572296
Query: 141 HPLWKWMKIQPKGKGILG 158
HPLWKWMK+QPKG+GILG
Sbjct: 572297 HPLWKWMKVQPKGRGILG 572350
Score = 69.3 bits (168), Expect(2) = 2e-59
Identities = 30/32 (93%), Positives = 31/32 (96%)
Frame = +3
Query: 29 CASRDDWRCARSMHEFSAKDIDGHMVNLDKYR 60
CASRDDWRCA+SMHEFSAKDIDG MVNLDKYR
Sbjct: 571764 CASRDDWRCAQSMHEFSAKDIDGSMVNLDKYR 571859
Score = 48.9 bits (115), Expect = 2e-04
Identities = 21/22 (95%), Positives = 22/22 (100%)
Frame = +2
Query: 167 KFLIDKNGCVVKRYGPMEEPLV 188
+FLIDKNGCVVKRYGPMEEPLV
Sbjct: 572738 QFLIDKNGCVVKRYGPMEEPLV 572803
>gi|472269405|gb|KB715284.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00578, whole genome
shotgun sequence
Length = 1301906
Score = 132 bits (333), Expect = 8e-30
Identities = 74/149 (49%), Positives = 90/149 (60%)
Frame = -3
Query: 28 MCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLH 87
+C S + W CA SMHE SA+DI G ++NLDK+ GFVCI+TNVASQ K T L
Sbjct: 744123 VCTSWEVWHCAHSMHELSARDIKGSIINLDKHWGFVCIMTNVASQ*DKQM*--TILG*PA 743950
Query: 88 ARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWM 147
R + FP +P EFAAGYN+K M + +C NGD+ H LWKW+
Sbjct: 743949 CRIS*LIFTDPGFPLQVVQGAKPRDYCRDYEFAAGYNIKLCMCN-VCENGDNDHLLWKWI 743773
Query: 148 KIQPKGKGILGNAIKWNFTKFLIDKNGCV 176
K+QPKG+ IL N KWNFTKF IDKN V
Sbjct: 743772 KVQPKGRRILENTTKWNFTKFFIDKNSSV 743686
>gi|472268596|gb|KB716061.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold01355, whole genome
shotgun sequence
Length = 634616
Score = 95.1 bits (235), Expect(3) = 9e-28
Identities = 49/96 (51%), Positives = 62/96 (64%)
Frame = +1
Query: 75 KTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKIC 134
+T+ N T+L+D A YAE G LAFPC+ +Q+PGSN ++ + A G VKF SKI
Sbjct: 593641 QTDTNCTKLLDPQAPYAESGSWSLAFPCDHPERQQPGSNVQMGQSADGSKVKFHEHSKI* 593820
Query: 135 VNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLI 170
V + HPLWK M+ QPKG + GNAIKW FTK I
Sbjct: 593821 VTREVTHPLWKGMEAQPKGWSMQGNAIKWTFTKIHI 593928
Score = 43.9 bits (102), Expect(3) = 9e-28
Identities = 25/49 (51%), Positives = 29/49 (59%)
Frame = +2
Query: 27 TMCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQXGK 75
TM S D CA +H+FSAKDI G M NL + + CIVTN Q GK
Sbjct: 593504 TM*VSSVDGHCAHPVHQFSAKDIGGDMFNL--HNLYFCIVTNRP*QRGK 593644
Score = 28.1 bits (61), Expect(3) = 9e-28
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +3
Query: 175 CVVKRYGPMEEPLVIEKDLP 194
CVV R P E PLVI+K+LP
Sbjct: 593940 CVVTR*CPTEGPLVIKKELP 593999
>gi|472269737|gb|KB714952.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00246, whole genome
shotgun sequence
Length = 1829979
Score = 81.3 bits (199), Expect = 3e-14
Identities = 41/86 (47%), Positives = 54/86 (62%), Gaps = 1/86 (1%)
Frame = +2
Query: 63 VCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-A 121
V +V NVAS+ G T+ +Y L L +LAFPCNQFG+QEP +N+EI+ FA
Sbjct: 1334972 VSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDTNKEIESFARR 1335151
Query: 122 GYNVKFDMFSKICVNGDDAHPLWKWM 147
Y V F MFSK+ V G AHP +K++
Sbjct: 1335152 TYGVSFPMFSKVAVTGTGAHPAFKYL 1335229
>gi|472269606|gb|KB715083.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00377, whole genome
shotgun sequence
Length = 1354867
Score = 70.5 bits (171), Expect = 5e-11
Identities = 35/86 (40%), Positives = 51/86 (59%), Gaps = 1/86 (1%)
Frame = +3
Query: 63 VCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAA- 121
V +V NVAS T+ NY L +LH + +LAFPCNQFG+ EP ++E+ FA
Sbjct: 621921 VALVVNVASDCQLTDRNYLALQELHKEFGPFHFSVLAFPCNQFGESEPRPSKEVVSFARN 622100
Query: 122 GYNVKFDMFSKICVNGDDAHPLWKWM 147
Y V F +F KI + G +A P ++++
Sbjct: 622101 NYGVTFPIFHKIKILGSEADPAFRFL 622178
>gi|472268977|gb|KB715680.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00974, whole genome
shotgun sequence
Length = 858563
Score = 60.8 bits (146), Expect = 4e-08
Identities = 31/75 (41%), Positives = 46/75 (61%)
Frame = +1
Query: 38 ARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRI 97
A+S ++ SA +DG V+ + +RG ++ NVAS G T ++TQL +L R+ L +
Sbjct: 331960 AKSFYDLSATSLDGEKVDFNTFRGRAVLIENVASL*GTTSRDFTQLNELQCRFPR-RLVV 332136
Query: 98 LAFPCNQFGKQEPGS 112
L FPCNQFG Q G+
Sbjct: 332137 LGFPCNQFGHQVRGA 332181
Score = 37.7 bits (86), Expect = 0.37
Identities = 40/145 (27%), Positives = 57/145 (39%), Gaps = 29/145 (20%)
Frame = +2
Query: 78 VNYTQLVDLHARYAECGLRILAFPCNQ------FGKQEPGSNEEIKEF------AAGYNV 125
V TQ + R E L IL FP F E NEEI G+
Sbjct: 334397 VEATQKRMILGREGERFLWILHFPLPSSMLFVFFLP*ENCQNEEILNSLKYVRPGGGFQP 334576
Query: 126 KFDMFSKICVNGDDAHPLWKWMK------------IQPKGKGILGNAIK-----WNFTKF 168
F + K VNG + HP++ ++K + K I+ + ++ WNF KF
Sbjct: 334577 TFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKFIIWSPVRRSDVAWNFEKF 334756
Query: 169 LIDKNGCVVKRYGPMEEPLVIEKDL 193
LI G +RY + + IE D+
Sbjct: 334757 LIGPEGEPFRRYSRTFQTINIEPDI 334831
>gi|472264638|gb|KB720019.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold05313, whole genome
shotgun sequence
Length = 66686
Score = 59.7 bits (143), Expect = 9e-08
Identities = 37/91 (40%), Positives = 50/91 (54%), Gaps = 9/91 (9%)
Frame = -1
Query: 27 TMCAS--------RDDWRCARSMHEFSAKDI-DGHMVNLDKYRGFVCIVTNVASQXGKTE 77
TMCA+ D RS++ FSA+ + G ++L RG V ++ NVAS G T
Sbjct: 62102 TMCAAPLAAAAAVADAAAAPRSVYAFSARPLAGGEPLSLGSLRGKVLLIENVASL*GTTV 61923
Query: 78 VNYTQLVDLHARYAECGLRILAFPCNQFGKQ 108
+YTQ+ +L R GL +L FPCNQFG Q
Sbjct: 61922 RDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 61830
Score = 48.1 bits (113), Expect = 3e-04
Identities = 36/115 (31%), Positives = 49/115 (42%), Gaps = 23/115 (20%)
Frame = -1
Query: 102 CNQFGKQEPGSNEEIKEF------AAGYNVKFDMFSKICVNGDDAHPLWKWMK------- 148
C+ F QE NEEI G+ F +F K VNG AHPL+ +++
Sbjct: 61628 CSPF-PQENAKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLRESLPAPS 61452
Query: 149 -------IQPK---GKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDL 193
PK + N I WNF KFL+ +G V+RY + IE D+
Sbjct: 61451 DDATALMTDPKFITWSPVCRNDIAWNFEKFLVGPDGVPVRRYSRRFPTINIEPDI 61287
>gi|472268783|gb|KB715874.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold01168, whole genome
shotgun sequence
Length = 709232
Score = 35.8 bits (81), Expect(2) = 2e-05
Identities = 17/24 (70%), Positives = 18/24 (75%)
Frame = +3
Query: 38 ARSMHEFSAKDIDGHMVNLDKYRG 61
A MHEFSAKD +G MVNLDK G
Sbjct: 154650 AHCMHEFSAKDREGCMVNLDKLLG 154721
Score = 35.0 bits (79), Expect(2) = 2e-05
Identities = 15/28 (53%), Positives = 22/28 (78%)
Frame = +1
Query: 62 FVCIVTNVASQXGKTEVNYTQLVDLHAR 89
F+ ++T+ A Q GKT+++ TQLVDLH R
Sbjct: 154735 FMRLITHKAEQ*GKTDISNTQLVDLHVR 154818
>gi|472269128|gb|KB715529.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00823, whole genome
shotgun sequence
Length = 893735
Score = 45.8 bits (107), Expect = 0.001
Identities = 21/36 (58%), Positives = 23/36 (63%)
Frame = +3
Query: 81 TQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEI 116
T+L L GL +L FPCNQFGKQEPG N EI
Sbjct: 523170 TELNALQEELKPLGLVVLGFPCNQFGKQEPGENSEI 523277
Score = 42.7 bits (99), Expect = 0.011
Identities = 17/23 (73%), Positives = 19/23 (82%)
Frame = -2
Query: 94 GLRILAFPCNQFGKQEPGSNEEI 116
G+ +L FPCNQFGKQEPG N EI
Sbjct: 487768 GVVVLGFPCNQFGKQEPGKNSEI 487700
>gi|472269970|gb|KB714719.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00013, whole genome
shotgun sequence
Length = 6116989
Score = 44.7 bits (104), Expect = 0.003
Identities = 20/28 (71%), Positives = 22/28 (78%)
Frame = +1
Query: 158 GNAIKWNFTKFLIDKNGCVVKRYGPMEE 185
G + + FTKFLIDKN CVVKRYGP EE
Sbjct: 4999621 GLFLHFYFTKFLIDKNSCVVKRYGPTEE 4999704
>gi|472268639|gb|KB716018.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold01312, whole genome
shotgun sequence
Length = 700520
Score = 44.3 bits (103), Expect = 0.004
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = +1
Query: 94 GLRILAFPCNQFGKQEPGSNEEI 116
GL IL FPCNQFGKQEPG N EI
Sbjct: 633880 GLVILGFPCNQFGKQEPGENSEI 633948
>gi|472269781|gb|KB714908.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold00202, whole genome
shotgun sequence
Length = 2068787
Score = 41.2 bits (95), Expect = 0.033
Identities = 35/100 (35%), Positives = 45/100 (45%), Gaps = 6/100 (6%)
Frame = -1
Query: 51 GHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEP 110
G ++L RG V + NVAS G +YTQ+ L R GL +L F + F QE
Sbjct: 965984 GEPMSLGCLRGKVLLFQNVASL*GPKVWDYTQMNQLQRRLRPQGLVVLGFRRHHFRHQEN 965805
Query: 111 GSNEEIKEF-----AAGY-NVKFDMFSKICVNGDDAHPLW 144
+EEI A G+ K VNG AHPL+
Sbjct: 965804 AKSEEILNSLEHVPARGWLQPNLSPSEKGEVNGVQAHPLF 965685
>gi|472268341|gb|KB716316.1| Leptonychotes weddellii isolate WS11-02
unplaced genomic scaffold scaffold01610, whole genome
shotgun sequence
Length = 611726
Score = 33.5 bits (75), Expect = 6.9
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = +2
Query: 108 QEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPL 143
+E G N+ I + YNV F+ F ICV +HPL
Sbjct: 225212 REGGKNKSISTISFIYNVSFEKFHTICVGRQISHPL 225319
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge
nome.fa
Posted date: Oct 18, 2013 6:11 PM
Number of letters in database: 3,087,506,849
Number of sequences in database: 5788
Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge
nome.fa.01
Posted date: Oct 18, 2013 6:11 PM
Number of letters in database: 69,379,310
Number of sequences in database: 10,922
Lambda K H
0.324 0.140 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 782,978,298
Number of Sequences: 16710
Number of extensions: 11057301
Number of successful extensions: 34522
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 444
Number of HSP's successfully gapped in prelim test: 251
Number of HSP's that attempted gapping in prelim test: 32589
Number of HSP's gapped (non-prelim): 5320
length of query: 197
length of database: 1,052,295,386
effective HSP length: 125
effective length of query: 72
effective length of database: 1,050,206,636
effective search space: 75614877792
effective search space used: 75614877792
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 74 (33.1 bits)