TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000016_2.0 # Protein # Iodothyronine deiodinase 3 (DI3) # Human (Homo sapiens) (278 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472268823|gb|KB715834.1| Leptonychotes weddellii isolate WS11... 527 e-148 gi|472269539|gb|KB715150.1| Leptonychotes weddellii isolate WS11... 139 2e-31 gi|472269548|gb|KB715141.1| Leptonychotes weddellii isolate WS11... 129 2e-28 gi|472269059|gb|KB715598.1| Leptonychotes weddellii isolate WS11... 68 5e-10 >gi|472268823|gb|KB715834.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01128, whole genome shotgun sequence Length = 719466 Score = 527 bits (1358), Expect = e-148 Identities = 251/266 (94%), Positives = 261/266 (98%) Frame = -3 Query: 13 CAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDP 72 CAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEP VEL+S+GEEVPPDDP Sbjct: 719185 CAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPAVELDSDGEEVPPDDP 719006 Query: 73 PICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQHILDYAQGN 132 P+CVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLP+GFQ+QHILDYA+GN Sbjct: 719005 PVCVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPNGFQNQHILDYARGN 718826 Query: 133 RPLVLNFGSCTXPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQ 192 RPLVLNFGSCT PPFMARMSAF RLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPY IPQ Sbjct: 718825 RPLVLNFGSCT*PPFMARMSAFHRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYSIPQ 718646 Query: 193 HRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPD 252 HRSLEDRVSAA+VLQQGAP C+LVLDTMANSSSSAYGAYFERLYVIQ+GTIMYQGGRGPD Sbjct: 718645 HRSLEDRVSAAQVLQQGAPSCSLVLDTMANSSSSAYGAYFERLYVIQNGTIMYQGGRGPD 718466 Query: 253 GYQVSELRTWLERYDEQLHGARPRRV 278 GYQVSELRTWLERYDEQLHGA+PRRV Sbjct: 718465 GYQVSELRTWLERYDEQLHGAQPRRV 718388 >gi|472269539|gb|KB715150.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00444, whole genome shotgun sequence Length = 1362633 Score = 139 bits (349), Expect = 2e-31 Identities = 80/191 (41%), Positives = 111/191 (58%), Gaps = 24/191 (12%) Frame = +2 Query: 99 FFKQAHEGGPAPNSEVV------------------LPDGFQSQHILDYAQGNRPLVLNFG 140 F Q G APNS VV + DG + H+LD+A RPLV+NFG Sbjct: 1059176 FHHQVKLGEDAPNSSVVHVSNSEGGDNSRNGAQVKMVDGAEC-HLLDFASPERPLVVNFG 1059352 Query: 141 SCTXPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGW-VTTDS--PYIIPQHRSLE 197 S T PPF +++ AF +LV ++ DFL++YI+EAHPSDGW V DS + + +HR+ E Sbjct: 1059353 SAT*PPFTSQLPAFSKLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSLSFEVKKHRNQE 1059532 Query: 198 DRVSAARVLQQG---APGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGY 254 DR +AA L + P C +V D M N+++ AYG FER+ ++Q I Y GG+GP Y Sbjct: 1059533 DRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFCY 1059712 Query: 255 QVSELRTWLER 265 + E+R WLE+ Sbjct: 1059713 NLQEVRRWLEK 1059745 >gi|472269548|gb|KB715141.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00435, whole genome shotgun sequence Length = 1363233 Score = 129 bits (323), Expect = 2e-28 Identities = 78/209 (37%), Positives = 117/209 (55%), Gaps = 24/209 (11%) Frame = +1 Query: 81 RLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVV------------------LPDGFQS 122 R+ T + +W D +KQ G APNS VV + DG + Sbjct: 833800 RMLTSEGMSCIWKSFLPDAYKQVTLGEDAPNSSVVHVSYSEGGDNSRNGAQVKMVDGAK- 833976 Query: 123 QHILDYAQGNRPLVLNFGSCTXPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGW- 181 H+LD+A RPLV+NFGS + PPF +++S +LV ++ DFL++YI EAHPSD W Sbjct: 833977 YHLLDFASPQRPLVVNFGSAS*PPFTSQLSYLSKLVEEFPSVADFLLVYINEAHPSDSWA 834156 Query: 182 VTTDS--PYIIPQHRSLEDRVSAA-RVLQQGA--PGCALVLDTMANSSSSAYGAYFERLY 236 V DS + + +H++ ED + A ++L+Q + P C +V D M N+++ AYG FER+ Sbjct: 834157 VPGDSSLSFEVKKHQNQEDCCATAHQLLEQFSLPPQC*VVTDHMGNNANVAYGVGFERVC 834336 Query: 237 VIQSGTIMYQGGRGPDGYQVSELRTWLER 265 ++Q I Y GG+GP Y + E+ WLE+ Sbjct: 834337 IVQRQKIAYLGGKGPFYYNLQEVWHWLEK 834423 >gi|472269059|gb|KB715598.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00892, whole genome shotgun sequence Length = 851605 Score = 68.2 bits (165), Expect = 5e-10 Identities = 35/73 (47%), Positives = 48/73 (65%) Frame = +3 Query: 178 SDGWVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYV 237 +DGW ++ I HR+L+DR+ AAR+L +P C +V+DTM N SS Y A ERLYV Sbjct: 589107 ADGWAFKNNVNI-RTHRNLQDRLQAARLLLDRSPQCPVVVDTMENQSSQRYAALPERLYV 589283 Query: 238 IQSGTIMYQGGRG 250 +Q G I+Y+ RG Sbjct: 589284 LQEGRILYKVTRG 589322 Score = 63.5 bits (153), Expect = 1e-08 Identities = 30/48 (62%), Positives = 34/48 (70%) Frame = +1 Query: 131 GNRPLVLNFGSCTXPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPS 178 GNRPLVLNFGSCT P F+ + F+RL + DFLIIYIEEAH S Sbjct: 583999 GNRPLVLNFGSCT*PSFLFKFDQFKRLTEDFCSTADFLIIYIEEAHAS 584142 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.322 0.139 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,225,764,752 Number of Sequences: 16710 Number of extensions: 22412710 Number of successful extensions: 80733 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 686 Number of HSP's successfully gapped in prelim test: 514 Number of HSP's that attempted gapping in prelim test: 74904 Number of HSP's gapped (non-prelim): 17775 length of query: 278 length of database: 1,052,295,386 effective HSP length: 130 effective length of query: 148 effective length of database: 1,050,123,086 effective search space: 155418216728 effective search space used: 155418216728 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 76 (33.9 bits)