TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000011_2.0 # Protein # Iodothyronine deiodinase 2 (DI2) # Human (Homo sapiens) (309 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472269548|gb|KB715141.1| Leptonychotes weddellii isolate WS11... 368 e-100 gi|472269539|gb|KB715150.1| Leptonychotes weddellii isolate WS11... 357 7e-97 gi|472268823|gb|KB715834.1| Leptonychotes weddellii isolate WS11... 141 6e-32 gi|472269059|gb|KB715598.1| Leptonychotes weddellii isolate WS11... 62 6e-08 gi|472268604|gb|KB716053.1| Leptonychotes weddellii isolate WS11... 36 3.4 gi|472268952|gb|KB715705.1| Leptonychotes weddellii isolate WS11... 36 3.4 gi|472268223|gb|KB716434.1| Leptonychotes weddellii isolate WS11... 35 7.7 gi|472269652|gb|KB715037.1| Leptonychotes weddellii isolate WS11... 35 7.7 >gi|472269548|gb|KB715141.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00435, whole genome shotgun sequence Length = 1363233 Score = 368 bits (945), Expect = e-100 Identities = 191/268 (71%), Positives = 202/268 (75%) Frame = +1 Query: 34 LKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQLNCPPSGFSKDGHILXLVY 93 L HVVLLLS SKST GEWRRMLTSEG+ C+WKSFL DAYKQ Sbjct: 833743 LGHVVLLLSHSKSTGGEWRRMLTSEGMSCIWKSFLPDAYKQ------------------- 833865 Query: 94 EAYKSRLLVYSHLDLWMVKLGEDAPNSSVVHVSSTEGGDNSGNGTQEKIAEGATCHLLDF 153 V LGEDAPNSSVVHVS +EGGDNS NG Q K+ +GA HLLDF Sbjct: 833866 -----------------VTLGEDAPNSSVVHVSYSEGGDNSRNGAQVKMVDGAKYHLLDF 833994 Query: 154 ASPERPLVVNFGSATXPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSS 213 ASP+RPLVVNFGSA+ PPFTSQL KLVEEF SVADFLLVYI+EAHPSD WA+PGDSS Sbjct: 833995 ASPQRPLVVNFGSAS*PPFTSQLSYLSKLVEEFPSVADFLLVYINEAHPSDSWAVPGDSS 834174 Query: 214 LSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQK 273 LSFEVKKHQNQED CA A QLLE+FSLPPQC VV D M NNAN+AYGV FERVCIVQRQK Sbjct: 834175 LSFEVKKHQNQEDCCATAHQLLEQFSLPPQC*VVTDHMGNNANVAYGVGFERVCIVQRQK 834354 Query: 274 IAYLGGKGPFSYNLQEVRHWLEKNFSKR 301 IAYLGGKGPF YNLQEV HWLEKNFSKR Sbjct: 834355 IAYLGGKGPFYYNLQEVWHWLEKNFSKR 834438 >gi|472269539|gb|KB715150.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00444, whole genome shotgun sequence Length = 1362633 Score = 357 bits (915), Expect = 7e-97 Identities = 173/191 (90%), Positives = 181/191 (94%) Frame = +2 Query: 111 VKLGEDAPNSSVVHVSSTEGGDNSGNGTQEKIAEGATCHLLDFASPERPLVVNFGSATXP 170 VKLGEDAPNSSVVHVS++EGGDNS NG Q K+ +GA CHLLDFASPERPLVVNFGSAT P Sbjct: 1059188 VKLGEDAPNSSVVHVSNSEGGDNSRNGAQVKMVDGAECHLLDFASPERPLVVNFGSAT*P 1059367 Query: 171 PFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRCAA 230 PFTSQLPAF KLVEEFSSVADFLLVYIDEAHPSDGWA+PGDSSLSFEVKKH+NQEDRCAA Sbjct: 1059368 PFTSQLPAFSKLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSLSFEVKKHRNQEDRCAA 1059547 Query: 231 AQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQEV 290 A QLLERFSLPPQC+VVADRMDNNAN+AYGVAFERVCIVQRQKIAYLGGKGPF YNLQEV Sbjct: 1059548 AHQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFCYNLQEV 1059727 Query: 291 RHWLEKNFSKR 301 R WLEKNFSKR Sbjct: 1059728 RRWLEKNFSKR 1059760 Score = 159 bits (402), Expect = 2e-37 Identities = 77/85 (90%), Positives = 82/85 (96%) Frame = +2 Query: 1 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGL 60 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEG+ Sbjct: 1050701 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGM 1050880 Query: 61 RCVWKSFLLDAYKQLNCPPSGFSKD 85 RC+WKSFLLDAYKQ++C GFS+D Sbjct: 1050881 RCIWKSFLLDAYKQVSCSDRGFSRD 1050955 >gi|472268823|gb|KB715834.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01128, whole genome shotgun sequence Length = 719466 Score = 141 bits (355), Expect = 6e-32 Identities = 100/290 (34%), Positives = 142/290 (48%), Gaps = 5/290 (1%) Frame = -3 Query: 12 LQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDA 71 L + P F L L D + + KH L R + + E L S+G Sbjct: 719164 LVLFPRFLGTAFMLWLLDFLCIRKH---FLGRRRRGQPEPAVELDSDGEEV--------- 719021 Query: 72 YKQLNCPPSGFSKDGHILXL-----VYEAYKSRLLVYSHLDLWMVKLGEDAPNSSVVHVS 126 + PP S D + L V+ K +H G APNS VV Sbjct: 719020 --PPDDPPVCVSDDNRLCTLASLKAVWHGQKLDFFKQAHE-------GGPAPNSEVV--- 718877 Query: 127 STEGGDNSGNGTQEKIAEGATCHLLDFASPERPLVVNFGSATXPPFTSQLPAFRKLVEEF 186 NG Q + H+LD+A RPLV+NFGS T PPF +++ AF +LV ++ Sbjct: 718876 -------LPNGFQNQ-------HILDYARGNRPLVLNFGSCT*PPFMARMSAFHRLVTKY 718739 Query: 187 SSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRV 246 DFL++YI+EAHPSDGW + DS + + +H++ EDR +AAQ L + P C + Sbjct: 718738 QRDVDFLIIYIEEAHPSDGW-VTTDS--PYSIPQHRSLEDRVSAAQVLQQG---APSCSL 718577 Query: 247 VADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEK 296 V D M N+++ AYG FER+ ++Q I Y GG+GP Y + E+R WLE+ Sbjct: 718576 VLDTMANSSSSAYGAYFERLYVIQNGTIMYQGGRGPDGYQVSELRTWLER 718427 >gi|472269059|gb|KB715598.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00892, whole genome shotgun sequence Length = 851605 Score = 61.6 bits (148), Expect = 6e-08 Identities = 29/59 (49%), Positives = 39/59 (66%) Frame = +1 Query: 155 SPERPLVVNFGSATXPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSS 213 S RPLV+NFGS T P F + F++L E+F S ADFL++YI+EAH S +P +S Sbjct: 583996 SGNRPLVLNFGSCT*PSFLFKFDQFKRLTEDFCSTADFLIIYIEEAHASGTERLPAPAS 584172 Score = 51.6 bits (122), Expect = 6e-05 Identities = 30/82 (36%), Positives = 46/82 (56%), Gaps = 2/82 (2%) Frame = +3 Query: 203 SDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVA 262 +DGWA + + ++ H+N +DR AA+ LL+R PQC VV D M+N ++ Y Sbjct: 589107 ADGWAFKNNVN----IRTHRNLQDRLQAARLLLDR---SPQCPVVVDTMENQSSQRYAAL 589265 Query: 263 FERVCIVQRQKIAY--LGGKGP 282 ER+ ++Q +I Y G GP Sbjct: 589266 PERLYVLQEGRILYKVTRGTGP 589331 >gi|472268604|gb|KB716053.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01347, whole genome shotgun sequence Length = 715730 Score = 35.8 bits (81), Expect = 3.4 Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 5/63 (7%) Frame = +1 Query: 9 LITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTS-----EGLRCV 63 L + L V+F +CL ++ + S+ L K + LL R ++RG RR S +G +C Sbjct: 546391 LTFISSLSVYFHSCLMVSFHFSLSLFKDFIYLLDRESTSRGSGRRKRRSRLPAEQGAQCA 546570 Query: 64 WKS 66 +S Sbjct: 546571 ARS 546579 >gi|472268952|gb|KB715705.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00999, whole genome shotgun sequence Length = 826042 Score = 35.8 bits (81), Expect = 3.4 Identities = 22/72 (30%), Positives = 33/72 (45%) Frame = +3 Query: 171 PFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRCAA 230 P +S P+F L + FS + DFLL + P+ + PG S + K+ + C Sbjct: 590124 PLSSSTPSFPPLSQTFSPLPDFLLGVLS---PTKSFTSPGQSPTPYTNKRQMEKCCACPG 590294 Query: 231 AQQLLERFSLPP 242 +L SLPP Sbjct: 590295 NIRLNRTSSLPP 590330 >gi|472268223|gb|KB716434.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01728, whole genome shotgun sequence Length = 546042 Score = 34.7 bits (78), Expect = 7.7 Identities = 20/58 (34%), Positives = 29/58 (50%) Frame = +1 Query: 16 PVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYK 73 P+FF +CL LA L+ L+ R++ + R +L GLRC WKS Y+ Sbjct: 320704 PIFFWSCLPLA*TRGEKLVAWTGLVPERTREKWADLRHVLFLTGLRCPWKSSQTQYYR 320877 >gi|472269652|gb|KB715037.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00331, whole genome shotgun sequence Length = 1609658 Score = 34.7 bits (78), Expect = 7.7 Identities = 22/63 (34%), Positives = 34/63 (53%), Gaps = 3/63 (4%) Frame = -1 Query: 23 LFLALYDSVILLKHVVLLLSRSKSTRGEWRR-MLTSEGLRCV--WKSFLLDAYKQLNCPP 79 +F + SVI H+ + SK R +WR+ LTS C+ ++ F+L + K+ NCP Sbjct: 833999 IFETSHSSVIFRVHLKMYFGHSKDNRVKWRKSCLTSIWYNCLLHYRLFVLQSLKK-NCPF 833823 Query: 80 SGF 82 GF Sbjct: 833822 PGF 833814 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.321 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,279,799,241 Number of Sequences: 16710 Number of extensions: 20473967 Number of successful extensions: 84254 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 755 Number of HSP's successfully gapped in prelim test: 552 Number of HSP's that attempted gapping in prelim test: 76841 Number of HSP's gapped (non-prelim): 21672 length of query: 309 length of database: 1,052,295,386 effective HSP length: 131 effective length of query: 178 effective length of database: 1,050,106,376 effective search space: 186918934928 effective search space used: 186918934928 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 77 (34.3 bits)