TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000002_2.0 # Protein # Iodothyronine deiodinase 1 (DI1) # Human (Homo sapiens) (249 letters) Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/geno me.fa 16,710 sequences; 3,156,886,159 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|472269059|gb|KB715598.1| Leptonychotes weddellii isolate WS11... 158 2e-37 gi|472268823|gb|KB715834.1| Leptonychotes weddellii isolate WS11... 153 1e-35 gi|472269539|gb|KB715150.1| Leptonychotes weddellii isolate WS11... 139 1e-31 gi|472269548|gb|KB715141.1| Leptonychotes weddellii isolate WS11... 115 2e-24 gi|472267672|gb|KB716985.1| Leptonychotes weddellii isolate WS11... 36 1.8 >gi|472269059|gb|KB715598.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00892, whole genome shotgun sequence Length = 851605 Score = 158 bits (400), Expect = 2e-37 Identities = 75/113 (66%), Positives = 83/113 (73%) Frame = +2 Query: 1 MGLPQPGLWLKRXXXXXXXXXXXXXXXXXXXXFPDRVKRNILAMGEKTGMTRNPHFSHDN 60 MGLP+PGLWLKR FP+RVK++ILAM EK NPHFS+DN Sbjct: 576641 MGLPRPGLWLKRLWVLLQVAVQVAVGKVFLILFPERVKQHILAMNEK-----NPHFSYDN 576805 Query: 61 WIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVRLSGQRCNIWEFMQG 113 W PTF+S QYFWFVLKVRWQRLED TE GGLAPNCPVVRLSGQRC+IW+FMQG Sbjct: 576806 WAPTFYSMQYFWFVLKVRWQRLEDRTEPGGLAPNCPVVRLSGQRCHIWDFMQG 576964 Score = 122 bits (307), Expect = 1e-26 Identities = 58/72 (80%), Positives = 65/72 (90%) Frame = +3 Query: 160 SDGWAFKNNMDIRNHQNLQDRLQAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYII 219 +DGWAFKNN++IR H+NLQDRLQAA LLL RSPQCPVVVDTM+NQSSQ YAALPERLY++ Sbjct: 589107 ADGWAFKNNVNIRTHRNLQDRLQAARLLLDRSPQCPVVVDTMENQSSQRYAALPERLYVL 589286 Query: 220 QEGRILYKGKSG 231 QEGRILYK G Sbjct: 589287 QEGRILYKVTRG 589322 Score = 90.1 bits (222), Expect = 1e-16 Identities = 42/50 (84%), Positives = 45/50 (90%) Frame = +1 Query: 111 MQGNRPLVLNFGSCTXPSFMFKFDQFKRLIEDFSSIADFLVIYIEEAHAS 160 + GNRPLVLNFGSCT PSF+FKFDQFKRL EDF S ADFL+IYIEEAHAS Sbjct: 583993 VSGNRPLVLNFGSCT*PSFLFKFDQFKRLTEDFCSTADFLIIYIEEAHAS 584142 Score = 49.7 bits (117), Expect = 2e-04 Identities = 21/24 (87%), Positives = 23/24 (95%) Frame = +3 Query: 225 LYKGKSGPWNYNPEEVRAVLEKLH 248 L +GKSGPWNY+PEEVRAVLEKLH Sbjct: 591573 LLQGKSGPWNYHPEEVRAVLEKLH 591644 >gi|472268823|gb|KB715834.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold01128, whole genome shotgun sequence Length = 719466 Score = 153 bits (386), Expect = 1e-35 Identities = 86/192 (44%), Positives = 115/192 (59%), Gaps = 2/192 (1%) Frame = -3 Query: 57 SHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVRLSG-QRCNIWEFMQGNR 115 S DN + T S + W K+ + + GG APN VV +G Q +I ++ +GNR Sbjct: 718993 SDDNRLCTLASLKAVWHGQKLDFFK---QAHEGGPAPNSEVVLPNGFQNQHILDYARGNR 718823 Query: 116 PLVLNFGSCTXPSFMFKFDQFKRLIEDFSSIADFLVIYIEEAHASDGWAFKNN-MDIRNH 174 PLVLNFGSCT P FM + F RL+ + DFL+IYIEEAH SDGW ++ I H Sbjct: 718822 PLVLNFGSCT*PPFMARMSAFHRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYSIPQH 718643 Query: 175 QNLQDRLQAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYIIQEGRILYKGKSGPWN 234 ++L+DR+ AA +L +P C +V+DTM N SS Y A ERLY+IQ G I+Y+G GP Sbjct: 718642 RSLEDRVSAAQVLQQGAPSCSLVLDTMANSSSSAYGAYFERLYVIQNGTIMYQGGRGPDG 718463 Query: 235 YNPEEVRAVLEK 246 Y E+R LE+ Sbjct: 718462 YQVSELRTWLER 718427 >gi|472269539|gb|KB715150.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00444, whole genome shotgun sequence Length = 1362633 Score = 139 bits (350), Expect = 1e-31 Identities = 78/185 (42%), Positives = 105/185 (56%), Gaps = 25/185 (13%) Frame = +2 Query: 87 ELGGLAPNCPVVRLS------------------GQRCNIWEFMQGNRPLVLNFGSCTXPS 128 +LG APN VV +S G C++ +F RPLV+NFGS T P Sbjct: 1059191 KLGEDAPNSSVVHVSNSEGGDNSRNGAQVKMVDGAECHLLDFASPERPLVVNFGSAT*PP 1059370 Query: 129 FMFKFDQFKRLIEDFSSIADFLVIYIEEAHASDGWAFKN----NMDIRNHQNLQDRLQAA 184 F + F +L+E+FSS+ADFL++YI+EAH SDGWA + +++ H+N +DR AA Sbjct: 1059371 FTSQLPAFSKLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSLSFEVKKHRNQEDRCAAA 1059550 Query: 185 HLLLAR---SPQCPVVVDTMQNQSSQLYAALPERLYIIQEGRILYKGKSGPWNYNPEEVR 241 H LL R PQC VV D M N ++ Y ER+ I+Q +I Y G GP+ YN +EVR Sbjct: 1059551 HQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFCYNLQEVR 1059730 Query: 242 AVLEK 246 LEK Sbjct: 1059731 RWLEK 1059745 >gi|472269548|gb|KB715141.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold00435, whole genome shotgun sequence Length = 1363233 Score = 115 bits (289), Expect = 2e-24 Identities = 70/184 (38%), Positives = 97/184 (52%), Gaps = 25/184 (13%) Frame = +1 Query: 88 LGGLAPNCPVVRLS------------------GQRCNIWEFMQGNRPLVLNFGSCTXPSF 129 LG APN VV +S G + ++ +F RPLV+NFGS + P F Sbjct: 833872 LGEDAPNSSVVHVSYSEGGDNSRNGAQVKMVDGAKYHLLDFASPQRPLVVNFGSAS*PPF 834051 Query: 130 MFKFDQFKRLIEDFSSIADFLVIYIEEAHASDGWAFKN----NMDIRNHQNLQDRLQAAH 185 + +L+E+F S+ADFL++YI EAH SD WA + +++ HQN +D AH Sbjct: 834052 TSQLSYLSKLVEEFPSVADFLLVYINEAHPSDSWAVPGDSSLSFEVKKHQNQEDCCATAH 834231 Query: 186 LLLAR---SPQCPVVVDTMQNQSSQLYAALPERLYIIQEGRILYKGKSGPWNYNPEEVRA 242 LL + PQC VV D M N ++ Y ER+ I+Q +I Y G GP+ YN +EV Sbjct: 834232 QLLEQFSLPPQC*VVTDHMGNNANVAYGVGFERVCIVQRQKIAYLGGKGPFYYNLQEVWH 834411 Query: 243 VLEK 246 LEK Sbjct: 834412 WLEK 834423 >gi|472267672|gb|KB716985.1| Leptonychotes weddellii isolate WS11-02 unplaced genomic scaffold scaffold02279, whole genome shotgun sequence Length = 458611 Score = 36.2 bits (82), Expect = 1.8 Identities = 18/50 (36%), Positives = 26/50 (52%) Frame = +2 Query: 42 LAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGL 91 L GEKTG + F +NW FF + W L++ W+ L++ T L L Sbjct: 198293 LPRGEKTGNIASFGFLDENWAAAFFQNSHIWGFLEI-WKELKNKTILSPL 198439 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 3,087,506,849 Number of sequences in database: 5788 Database: /cursos/BI/genomes/project_2014/Leptonychotes_weddellii/ge nome.fa.01 Posted date: Oct 18, 2013 6:11 PM Number of letters in database: 69,379,310 Number of sequences in database: 10,922 Lambda K H 0.324 0.138 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,026,187,806 Number of Sequences: 16710 Number of extensions: 15095434 Number of successful extensions: 83536 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 777 Number of HSP's successfully gapped in prelim test: 448 Number of HSP's that attempted gapping in prelim test: 76479 Number of HSP's gapped (non-prelim): 20197 length of query: 249 length of database: 1,052,295,386 effective HSP length: 129 effective length of query: 120 effective length of database: 1,050,139,796 effective search space: 126016775520 effective search space used: 126016775520 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 76 (33.9 bits)