TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000636_2.0 # Protein # Selenoprotein J (SelJ) # Zebrafish
(Danio rerio)
         (342 letters)

Database: /cursos/BI/genomes/project_2014/Chelonia_mydas/genome.fa 
           140,022 sequences; 2,208,393,880 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|465958201|gb|KB567448.1| Chelonia mydas unplaced genomic scaf...    34   8.2  
gi|465984757|gb|KB524361.1| Chelonia mydas unplaced genomic scaf...    34   8.2  

>gi|465958201|gb|KB567448.1| Chelonia mydas unplaced genomic scaffold
              scaffold619, whole genome shotgun sequence
          Length = 860129

 Score = 34.3 bits (77), Expect = 8.2
 Identities = 35/124 (28%), Positives = 58/124 (46%), Gaps = 5/124 (4%)
 Frame = +2

Query: 173    LEKVEEAVRVTQNNDLCVAETLAAARFLEHFILNGPDPKALDAVLKQLSDPERKNPQELD 232
              LEK +   + + N     +ET   AR    FI  GP P  L  +     D  +K+P+E +
Sbjct: 343874 LEKGKRRTKGSLNRIFKPSETTRWARQ*GVFIFQGPKPFTLQTIGSHQRD--KKSPEE-E 344044

Query: 233    KAVIGHIHQVKDNLNKKPKELIPAVFPNTXGLPGAFQAAL-----HGVLTASSYEQAVRD 287
                V   + +++D+L++ P+ L  A+F  T G     ++       HG   ASS  Q +  
Sbjct: 344045 ARVRHFMSELRDSLSRLPEWLRQALF--TVGREAGTESHQKGL**HGGCDASSS*QPLSI 344218

Query: 288    TMSC 291
              ++SC
Sbjct: 344219 SLSC 344230


>gi|465984757|gb|KB524361.1| Chelonia mydas unplaced genomic scaffold
              scaffold231, whole genome shotgun sequence
          Length = 2205857

 Score = 34.3 bits (77), Expect = 8.2
 Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 2/62 (3%)
 Frame = -3

Query: 254    IPAVFPNTXGLPGAFQAALHGVLTASSYEQAVRDTMSCGGCTCSRSSFIGACIG--AQVG 311
              +P +F      P A    + G L   S E  +R  ++CG C C+  S  G+C+    QV 
Sbjct: 739641 LPLLFTALWSWPTALSPCIAGSLETVSLEAQLRLELACGVCLCNGPSNSGSCVNDIIQVS 739462

Query: 312    LE 313
              L+
Sbjct: 739461 LQ 739456


  Database: /cursos/BI/genomes/project_2014/Chelonia_mydas/genome.fa
    Posted date:  Oct 18, 2013  5:55 PM
  Number of letters in database: 2,208,393,880
  Number of sequences in database:  140,022
  
Lambda     K      H
   0.317    0.134    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 938,067,794
Number of Sequences: 140022
Number of extensions: 15741429
Number of successful extensions: 69738
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 4272
Number of HSP's successfully gapped in prelim test: 590
Number of HSP's that attempted gapping in prelim test: 9948
Number of HSP's gapped (non-prelim): 64951
length of query: 342
length of database: 736,131,293
effective HSP length: 130
effective length of query: 212
effective length of database: 717,928,433
effective search space: 152200827796
effective search space used: 152200827796
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 76 (33.9 bits)
