SELENOPROTEÍNAS DE Meleagris gallopavo
PROTEIN REPORT FOR Methionine-R-sufoxidereductase 3 (SelR3)
| DESCRIPCIÓN | Homólogo con cisteína. 1(+):35733089...35786063. |
| SECISearch | Sin resultados. |
| SELENOPROFILES | Encontrada. Elección: Exonerate. Elemento SECIS no predicho. |
| COMENTARIOS | Elección: Genewise. Ausencia de elementos SECIS. Vemos que en el alineamiento con SPP00000089 (homólogo Drosophila melanogaster), la predicción exoniza una región no exonizada en el resto. Viendo la predicción de Selenoprofiles, vemos que en la posición mencionada se reporta la presencia de un intrón. Predicción coincidente (Sel R3) con la anotación del gen en NCBI. |
1. ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
2. BEST PAIRWISE ALIGNMENT
4. RESULTADOS DEL Selenoprofiles
ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=91, Nseq=12, Len=377
SPP00000023_1.0 MSPRRT-------LPRPLSLCLSLCLCLCLAAAL--------------GSAQSGSCRDKK
SPP00000023_1.0.1.exonerate L-------------------CLWFVFCVCLQVVS--------------HAGNQGSCRDKK
SPP00000023_1.0.1.genewise I-----------------------------------------------SSPFAGSCRDKK
SPP00000089_1.0 --------------------------------------------------------MDNK
SPP00000089_1.0.1.exonerate ---------------------------------------------------------DKK
SPP00000089_1.0.1.genewise ---------------------------------------------------------DKK
SPP00000022_1.0 MGAGAETGRGQRAAPERAARLLWLLRGLTLGTAPRRAVRGQAGGGGPGTGPGLGEAGSLA
SPP00000022_1.0.1.exonerate ------------------------------------------------------------
SPP00000022_1.0.1.genewise ------------------------------------------------------------
SPP00000059_1.0 MTTKKF-------R-------------------M--------------EDVGLSKLKVEK
SPP00000059_1.0.1.exonerate ------------------------------------------------------------
SPP00000059_1.0.1.genewise ------------------------------------------------------------
SPP00000023_1.0 NCKVVFSQQELRKRLTPLQYHVTQEKGTESAFEGEYTHHKDPGIYKCVVCGTPL------
SPP00000023_1.0.1.exonerate DCKVVFSQEELRKRLTPLQYHVTQEKGTESAFEGEYTHHKAQGIYKCVVCGTPL------
SPP00000023_1.0.1.genewise DCKVVFSQEELRKRLTPLQYHVTQEKGTESAFEGEYTHHKAQGIYKCVVCGTPL------
SPP00000089_1.0 SEKVTVNKEELRKRLTPVQYQVTQEAGTERPFTGCYNKHYEKGVYQCIVCHQDL------
SPP00000089_1.0.1.exonerate DCKVVFSQEELRKRLTPLQYHVTQEKGTERPKSFAPLAQHLNSLLKHFLKANPLAKYIGL
SPP00000089_1.0.1.genewise DCKVVFSQEELRKRLTPLQYHVTQEKGTESAFEGEYTHHKAQGIYKCVVCGTPL------
SPP00000022_1.0 TCELPLAKSEWQKKLTPEQFYVTREKGTEPPFSGIYLNNKEAGMYHCVCCDSPL------
SPP00000022_1.0.1.exonerate ------------------------------------------------------------
SPP00000022_1.0.1.genewise ------------------------------------------------------------
SPP00000059_1.0 NPKD-VKQTEWKSVLPNEVYRVARESGTETPHTGGFNDHFEKGRYVCLCCGSEL------
SPP00000059_1.0.1.exonerate ------------------------------------------------------------
SPP00000059_1.0.1.genewise ------------------------------------------------------------
SPP00000023_1.0 ------------------------------------------------------------
SPP00000023_1.0.1.exonerate ------------------------------------------------------------
SPP00000023_1.0.1.genewise ------------------------------------------------------------
SPP00000089_1.0 ------------------------------------------------------------
SPP00000089_1.0.1.exonerate PLRGSTHIIKLKEYTNVLFVELLYSXNFPHKPNLTTNCQCQHGIKCSVKEVVPEVRNEVL
SPP00000089_1.0.1.genewise ------------------------------------------------------------
SPP00000022_1.0 ------------------------------------------------------------
SPP00000022_1.0.1.exonerate ------------------------------------------------------------
SPP00000022_1.0.1.genewise ------------------------------------------------------------
SPP00000059_1.0 ------------------------------------------------------------
SPP00000059_1.0.1.exonerate ------------------------------------------------------------
SPP00000059_1.0.1.genewise ------------------------------------------------------------
SPP00000023_1.0 -FKSETKFDSGSG----------------------------------------------W
SPP00000023_1.0.1.exonerate -FKSETKFDSNSG----------------------------------------------W
SPP00000023_1.0.1.genewise -FKSETKFDSNSG----------------------------------------------W
SPP00000089_1.0 -FSSETKYDSGCG----------------------------------------------W
SPP00000089_1.0.1.exonerate QLRSETKFDSNSESGVQEVLKLLQLLLEKRPTPTSPQPSFRLAFLLCDQSRYNYSHFFLW
SPP00000089_1.0.1.genewise -FKSETKFDSNSG----------------------------------------------W
SPP00000022_1.0 -FSSEKKYCSGTG----------------------------------------------W
SPP00000022_1.0.1.exonerate ------------------------------------------------------------
SPP00000022_1.0.1.genewise ------------------------------------------------------------
SPP00000059_1.0 -FNSDAKFWAGCG----------------------------------------------W
SPP00000059_1.0.1.exonerate --------LWGCG----------------------------------------------W
SPP00000059_1.0.1.genewise ------------G----------------------------------------------W
SPP00000023_1.0 PSFHDVINSE------A-ITFTDDFS-YGMHRVETSCSQCGAHLGHIFDDGPRP-TGKRY
SPP00000023_1.0.1.exonerate PSFYDVISPD------T-ITLTDDFS-YGMHRIEISCSQCGAHLGHIFDDGPRP-TGKRY
SPP00000023_1.0.1.genewise PSFYDVISPD------T-ITLTDDFS-YGMHRIEISCSQCGAHLGHIFDDGPRP-TGKRY
SPP00000089_1.0 PAFNDVLDKG------K-VTLHRDASIPGMVRTEVRCSRCSAHMGHVFDDGPPP-KHRRF
SPP00000089_1.0.1.exonerate DASDNIL--Q------S-VTLTDDFS-YGMHRIEISCSQCGAHLGHIFDDGPRP-TGKRY
SPP00000089_1.0.1.genewise PSFYDVISPD------T-ITLTDDFS-YGMHRIEISCSQCGAHLGHIFDDGPRP-TGKRY
SPP00000022_1.0 PSFSEAHGTSGSDESHTGILRRLDTS-LGSARTEVVCKQCEAHLGHVFPDGPGP-NGQRF
SPP00000022_1.0.1.exonerate -----------------------DFS-YGMHRIEISCSQCGAHLGHIFDDGPRP-TGKRY
SPP00000022_1.0.1.genewise --------------------------------------QCGAHLGHIFDDGPRP-TGKRY
SPP00000059_1.0 PAFSESVGQD------ANIVRIVDRS-HGMHRTEVRCKTCDAHLGHVFNDGPKETTGERY
SPP00000059_1.0.1.exonerate PSFYDVISPD------T-ITLTDDFS-YGMHRIEISCSQCGAHLGHIFDDGPRP-TGKRY
SPP00000059_1.0.1.genewise PSFYDVISPD------T-ITLTDDFS-YGMHRIEISCSQCGAHLGHIFDDGPRP-TGKRY
* **:**:* *** . .*:
SPP00000023_1.0 CINSAA------------------------------------------LSFTPADSSGTA
SPP00000023_1.0.1.exonerate CINSAS------------------------------------------LSFKPAEKNSAG
SPP00000023_1.0.1.genewise CINSAS------------------------------------------LSFKPAEKNSAG
SPP00000089_1.0 CINSASIDFVKKRIRTEVRCARCNAHMGHVFEDGPKPTRKRYCINSASIEFVNADPATSS
SPP00000089_1.0.1.exonerate CINSASLSFC-------------GAHLGHIFDDGPRPTGKRYCINSASLSFKPA------
SPP00000089_1.0.1.genewise CINSAS------------------------------------------LS----------
SPP00000022_1.0 CINSVA------------------------------------------LKFKPR------
SPP00000022_1.0.1.exonerate CINSAS------------------------------------------LSFK--------
SPP00000022_1.0.1.genewise CINSAS------------------------------------------LSFK--------
SPP00000059_1.0 CINSVC------------------------------------------MAFEKK------
SPP00000059_1.0.1.exonerate CINSAS------------------------------------------LSFK--------
SPP00000059_1.0.1.genewise CINSAS------------------------------------------LSFK--------
****.. :
SPP00000023_1.0 EGGSGVASPAQADKAEL
SPP00000023_1.0.1.exonerate E-GSGSTRPAQTGKAEL
SPP00000023_1.0.1.genewise E-GSGSTRPAQTGKAEL
SPP00000089_1.0 ---PPVATPTAAPIAQQ
SPP00000089_1.0.1.exonerate ----------------E
SPP00000089_1.0.1.genewise ----------------F
SPP00000022_1.0 ---------------KH
SPP00000022_1.0.1.exonerate ----------------P
SPP00000022_1.0.1.genewise ----------------P
SPP00000059_1.0 ----------------D
SPP00000059_1.0.1.exonerate -----------------
SPP00000059_1.0.1.genewise -----------------
......................................................................................................................................................................................................................................................
BEST PAIRWISE ALIGNMENT
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=98, Nseq=2, Len=192
SPP00000023_1.0 MSPRRTLPRPLSLCLSLCLCLCLAAALGSAQSGSCRDKKNCKVVFSQQELRKRLTPLQYH
SPP00000023_1.0.1.exonerate ------------LCLWFVFCVCLQVVSHAGNQGSCRDKKDCKVVFSQEELRKRLTPLQYH
*** : :*:** .. :.:.*******:*******:************
SPP00000023_1.0 VTQEKGTESAFEGEYTHHKDPGIYKCVVCGTPLFKSETKFDSGSGWPSFHDVINSEAITF
SPP00000023_1.0.1.exonerate VTQEKGTESAFEGEYTHHKAQGIYKCVVCGTPLFKSETKFDSNSGWPSFYDVISPDTITL
******************* *********************.******:***..::**:
SPP00000023_1.0 TDDFSYGMHRVETSCSQCGAHLGHIFDDGPRPTGKRYCINSAALSFTPADSSGTAEGGSG
SPP00000023_1.0.1.exonerate TDDFSYGMHRIEISCSQCGAHLGHIFDDGPRPTGKRYCINSASLSFKPAEKNSAGE-GSG
**********:* *****************************:***.**:...:.* ***
SPP00000023_1.0 VASPAQADKAEL
SPP00000023_1.0.1.exonerate STRPAQTGKAEL
: ***:.****
......................................................................................................................................................................................................................................................
RESULTADOS DEL Selenoprofiles
Output_id: SelR.4.cysteine
---------- ---------------
-Species Meleagris gallopavo -Taxid 9103
-Target /homes/users/U63748/gallopavo.fa
-Chromosome (+) 1
-Program exonerate
-Query name gi|119892367|ref|XP_875267.2| PREDICTED: similar to methionine sulfoxide reductase B3 isoform 4 [Bos taurus]
-Query range 13-191 length:191 coverage: 0.94
-Profile range 110-792 length:792 coverage: 0.86 sec_position: [448]
-Average sequence identity with profile: 0.342 (ignoring gaps: 0.47)
-State kept
------- alignment -------
Query LCLCLSL <---Intron---> CLAAAAARGAVQS <---Intron---> GSCRDKKNCKVVFSQQELRKRLTPLQYHVTQEKGTES <---Intron
|||||| < 41032bp > /| ||||||| / < 138555bp > |||||||/|||||||/||||||||||||||||||||| < 2467bp
Target LCLCLSP LVAVAAARGAVTA GSCRDKKDCKVVFSQEELRKRLTPLQYHVTQEKGTES
ctttctc cggggggcgggag gttagaagtaggttcggcaaccactctcgacgagaga
tgtgtcc ttctcccggctcc gcggaaaagatttcaaatgagtcctaaatcaaagcag
ttatcat ggtgttatatgag gatgtaactggctcggggggggacggctctggggaa
Query ---> AFEGEYTHHKDPGIYKCVVCGTPLFK <---Intron---> SETKFDSGSG <---Intron---> WPSFHDVINSDAITFTDDFSYGMHRVE
> |||||||||| |||||||||||||| < 10473bp > ||||||| || < 34946bp > ||||/|||/ | || ||||||||||/|
Target AFEGEYTHHKAQGIYKCVVCGTPLFK SETKFDSNSG WPSFYDVISPDTITLTDDFSYGMHRIE
gtgggtaccagcgatatggtgactta tgaatgtatg tctttggaacgaaacaggtttgaccag
ctagaacaaacagtaagttggcctta cacatacac ggcctaattgcactctcaatcagtagta
tctgggcattataaacattttattac gaaggtccca tgtccttgctctattcttttctggtgta
Query TSCSQ <---Intron---> CGAHLGHIFDDGPRPTGKRYUINSASLSFSPAESSGAQGDSAGGSPAPGDKTEL
|||| < 4621bp > |||||||||||||||||||| |||||||| ||| / | | || | ||
Target ISCSQ CGAHLGHIFDDGPRPTGKRYCINSASLSFKPAEKNSAGEGSGSTRPAQTGKAEL
attac tggccgcatgggcccagacttaatgttttacggaaaggggagaaccgcagaggc
tcgga ggcatgattaagcgccgagagtaccctctaccaaagcgaggggcgccacgacat
acctg ctaccgcttttattatcagccacttgaccataggcccgacttccttcaacatgg
*
------- positions -------
Exon 1 35553433 35553453
Exon 2 35594486 35594524
Exon 3 35733080 35733189
Exon 4 35735657 35735734
Exon 5 35746208 35746236
Exon 6 35781183 35781280
Exon 7 35785902 35786063
--------- SECIS ---------
None found
--------- 3' seq --------
Total sequence length available downstream >= 3000
Sequence until first stop codon:
TAG
*
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