SELENOPROTEÍNAS DE Meleagris gallopavo
PROTEIN REPORT FOR Selenoprotein I (Sel I)
| DESCRIPCIÓN | Homólogo de Sel I con la selenocisteína no alineada. 28(-):1112218...1109434. |
| SECISearch | Sin resultados. |
| SELENOPROFILES | Encontrada. Elección: Blast. Predicción elemento SECIS (chromosome:28 strand:- positions:1107472-1107578). |
| COMENTARIOS | Elección: Genewise. La predicción de Genewise es más corta que la de Exonerate pero la homología de las secuencias es mayor. Seguramente por ese motivo la predicción de Genewise obtuvo un mejor score. La proteína predicha por selenoprofiles tiene la misma longitud que la nuestra predicha por Genewise y ambas terminan antes de alinearse con la selenocisteína en la proteína homóloga. Tiene un elemento SECIS predicho por selenoprofiles. Podría tratarse de una pérdida del exon que contenía la selenocisteína (región 3') en un evento de duplicación por ejemplo. |
1. ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
2. BEST PAIRWISE ALIGNMENT
4. RESULTADOS DEL Selenoprofiles
ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=79, Nseq=9, Len=427
SPP00000088_1.0 MGCMRYLSEAHLRGFERYKYSSIDTSFLSVYVMHPFWNYCVKF----VPKWLAPNVLTFV
SPP00000088_1.0.28.exonerate ------LSKHQLKRLEEHKYQSAGRSLLE-PLMQGYWEWLVGR----VPAWIAPNLITII
SPP00000088_1.0.28.genewise ------LSKHQLKRLEEHKYQSAGRSLLE-PLMQGYWEWLVGR----VPAWIAPNLITII
SPP00000015_1.0 MAGYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKV----FPTWLAPNLITFS
SPP00000015_1.0.28.exonerate ------LSKHQLKRLEEHKYQSAGRSLLE-PLMQGYWEWLVGR----VPAWIAPNLITII
SPP00000015_1.0.28.genewise ------LSKHQLKRLEEHKYQSAGRSLLE-PLMQGYWEWLVGR----VPAWIAPNLITII
SPP00000111_1.0 MAGYEYVSPEQLSGFDKYKYSALDTNPLSLYIMHPFWNTIVKKKKQVFPTWLAPNLITFS
SPP00000111_1.0.28.exonerate ------------------------------------------------PAWIAPNLITII
SPP00000111_1.0.28.genewise ------LSKHQLKRLEEHKYQSAGRSLLE-PLMQGYWEWLVGR----VPAWIAPNLITII
* *:***::*:
SPP00000088_1.0 GFLMTVVNFILIAYYDWGFEAANSETGNTVPAWVWTVAAINILIYYNLDGMDGKQARRTG
SPP00000088_1.0.28.exonerate GLLINIFTTLLLVYYCPTATEQEIEARKGAPPWAYIACACGLFIYQSLDAIDGKQARRTN
SPP00000088_1.0.28.genewise GLLINIFTTLLLVYYCPTATEQ-------APPWAYIACACGLFIYQSLDAIDGKQARRTN
SPP00000015_1.0 GFLLVVFNFLLMAYFDPDFYASA-PGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTN
SPP00000015_1.0.28.exonerate GLLINIFTTLLLVYYCPTATEQA-DGRVPAPPWAYIACACGLFIYQSLDAIDGKQARRTN
SPP00000015_1.0.28.genewise GLLINIFTTLLLVYYCPTATEQ-------APPWAYIACACGLFIYQSLDAIDGKQARRTN
SPP00000111_1.0 GFMLLVFNFLLLTYFDPDFYASA-PGHKHVPDWVWIVVGILNFAAYTLDGVDGKQARRTN
SPP00000111_1.0.28.exonerate GLLINIFTTLLLVYYCPTATEQA-DGRVPAPPWAYIACACGLFIYQSLDAIDGKQARRTN
SPP00000111_1.0.28.genewise GLLINIFTTLLLVYYCPTATEQ-------APPWAYIACACGLFIYQSLDAIDGKQARRTN
*::: :.. :*:.*: .* *.: . . : .**.:********.
SPP00000088_1.0 TSGPLGELFDHGLDSYSAALIPIYLFSLFG--THDLPPIRMFFVIWNVFLNFYLTHVEKY
SPP00000088_1.0.28.exonerate SSTPLGELFDHGCDSLSTAV--FVVLGTCIAVQLGTNPDWMFFCCFAGTFMFYCAHWQTY
SPP00000088_1.0.28.genewise SSTPLGELFDHGCDSLSTGI----------------------------------------
SPP00000015_1.0 SSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSTGVSVFVLYLLLWVVLFSFILSHWEKY
SPP00000015_1.0.28.exonerate SSTPLGELFDHGCDSLSTVAAVFVVLGTCIAVQLGTNPDWMFFCCFAGTFMFYCAHWQTY
SPP00000015_1.0.28.genewise SSTPLGELFDHGCDSL--------------------------------------------
SPP00000111_1.0 SSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGPTGVSVFVLYLLLWVVLFSFILSHWEKY
SPP00000111_1.0.28.exonerate SSTPLGELFDHGCDSLSTVAAVFVVLGTCIAVQLGTNPDWMFFCCFAGTFMFYCAHWQTY
SPP00000111_1.0.28.genewise SSTPLGELFDHGCDSL--------------------------------------------
:* ********* **
SPP00000088_1.0 NTGVMFLPWGY-DFTMWGVSGMLFVATVFGPEM-YRFSIYGFTMANMFEF---VLIGSGM
SPP00000088_1.0.28.exonerate VSGTHTARHGL-CFKSSGVSSQVCAAVPGGSEM-AIIVCSGFTRPDLGVFASGR----AL
SPP00000088_1.0.28.genewise ------------------------------------------------------------
SPP00000015_1.0 NTGILFLPWGY-DISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLF---TAMIIGCAL
SPP00000015_1.0.28.exonerate VSGTLR--FGIFSLLEIAI-FIFHFSKLAALLTWHSSFIIHLQCKSVL---MGSLK--PK
SPP00000015_1.0.28.genewise ------------------------------------------------------------
SPP00000111_1.0 NTGVLFLPWGY-DISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLF---TAMIIGCAL
SPP00000111_1.0.28.exonerate VSGTLR--FGIFSLLEIAI-FIFHFSKLAALLTWHSSFIIHLQCKSVL---MGSLK--PK
SPP00000111_1.0.28.genewise ------------------------------------------------------------
SPP00000088_1.0 VSSHPI-IARNIYLSYKNKTGKMRPMWEMLRPFFAFVWLFVITVVWSFFSRNDVINKEPR
SPP00000088_1.0.28.exonerate LQSSS-LCCKAVLLPFKLRRGKPLPGTSVLSPFLHIGSVIALAAMIYKKSAVQLFEKHPC
SPP00000088_1.0.28.genewise ------------------------------------------------------------
SPP00000015_1.0 CVTLPM-SLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPR
SPP00000015_1.0.28.exonerate CLKKKINVLKCFFPSHKGEVVKLRSVWRGICGLFSSVMLFNITICWVLWARLKLFEKHPC
SPP00000015_1.0.28.genewise ------------------------------------------------------------
SPP00000111_1.0 CVTLPM-SLLNFFRSYKSNTLKHKSVYEAMVPFFSPCLLFTLCTVWILWSPSDILEIHPR
SPP00000111_1.0.28.exonerate CLKKKINVLKCFFPSHKGEVVKLRSGTSVLSPFLHIGSVIALAAMIYKKSAVQLFEKHPC
SPP00000111_1.0.28.genewise ------------------------------------------------------------
SPP00000088_1.0 ILWILYGTIFSNIACRLIVAQMSDTRCDAFNVLMWPLAATVGVCCFPYYQQVFDSDLTSD
SPP00000088_1.0.28.exonerate LYILTFGFVSAKITNKLVLFWLCVNHCHCWSVLMSPTSFVFTCPALLFLDQYFNSFIDEY
SPP00000088_1.0.28.genewise ------------------------------------------------------------
SPP00000015_1.0 VFYFMVGTAFANSTCQLIVCQMSSTRCPTLNWLLVPLFLVVLV--------V-NLGVASY
SPP00000015_1.0.28.exonerate LYILTFGFVSAKITNKLVVAHMTKSEMHLHDTAFI-------GPALLFLDQYFNSFIDEY
SPP00000015_1.0.28.genewise ------------------------------------------------------------
SPP00000111_1.0 IFYFMVGTAFANITCQLIVCQMSSTRCPTLNWLLLPLLLVVAA--------VIVGAATSR
SPP00000111_1.0.28.exonerate LYILTFGFVSAKITNKLVVAHMTK------------------------------------
SPP00000111_1.0.28.genewise ------------------------------------------------------------
SPP00000088_1.0 TERWILYGLTIFSTLAHWHYGYGVVSEMCDHFHIRCFKVRKSSSQ-SGSDITQLLQNNNK
SPP00000088_1.0.28.exonerate IVLWIALPLSIFSLFDLLRYCVSVCNQIAAHLHIHVFRIKSSST----------------
SPP00000088_1.0.28.genewise ------------------------------------------------------------
SPP00000015_1.0 VESILLYTLTTAFTLAHIHYGVRVVKQLSSHFQIYPFSLRKPNSDULGMEEKN-------
SPP00000015_1.0.28.exonerate IVLWIAL---IFSLFDLLRYCVSVCNQIAAHLHIHVFRIKSSS-----------------
SPP00000015_1.0.28.genewise ------------------------------------------------------------
SPP00000111_1.0 LESALLYTLTAAFTLAHIHYGVQVVKQLSRHFQIYPFSLRKPNSDULGMEEQN-------
SPP00000111_1.0.28.exonerate ------------------------------------------------------------
SPP00000111_1.0.28.genewise ------------------------------------------------------------
SPP00000088_1.0 IKPLKSH
SPP00000088_1.0.28.exonerate -----HS
SPP00000088_1.0.28.genewise ------V
SPP00000015_1.0 ----IGL
SPP00000015_1.0.28.exonerate ------T
SPP00000015_1.0.28.genewise ------S
SPP00000111_1.0 ----IGL
SPP00000111_1.0.28.exonerate ------S
SPP00000111_1.0.28.genewise ------S
......................................................................................................................................................................................................................................................
BEST PAIRWISE ALIGNMENT
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=94, Nseq=2, Len=397
SPP00000015_1.0 MAGYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLL
SPP00000015_1.0.28.genewise ------LSKHQLKRLEEHKYQSAGRSLLE-PLMQGYWEWLVGRVPAWIAPNLITIIGLLI
:* .** ::::**.:.. . *. :*: :*: :* .*:*:******: *:*:
SPP00000015_1.0 VVFNFLLMAYFDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPL
SPP00000015_1.0.28.genewise NIFTTLLLVYYCPTAT------EQAPPWAYIACACGLFIYQSLDAIDGKQARRTNSSTPL
:*. **:.*: * ::.* *.:*. . *: :**.:**************
SPP00000015_1.0 GELFDHGLDSWSCVYFVVTVYSIFGRGSTGVSVFVLYLLLWVVLFSFILSHWEKYNTGIL
SPP00000015_1.0.28.genewise GELFDHGCDSLS------------------------------------------------
******* ** *
SPP00000015_1.0 FLPWGYDISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCVTLPMSLL
SPP00000015_1.0.28.genewise ------------------------------------------------------------
SPP00000015_1.0 NFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAF
SPP00000015_1.0.28.genewise ------------------------------------------------------------
SPP00000015_1.0 ANSTCQLIVCQMSSTRCPTLNWLLVPLFLVVLVVNLGVASYVESILLYTLTTAFTLAHIH
SPP00000015_1.0.28.genewise ------------------------------------------------------------
SPP00000015_1.0 YGVRVVKQLSSHFQIYPFSLRKPNSDULGMEEKNIGL
SPP00000015_1.0.28.genewise -------------------------------------
......................................................................................................................................................................................................................................................
RESULTADOS DEL Selenoprofiles
Output_id: SelI.15.unaligned
---------- -----------------
-Species Meleagris gallopavo -Taxid 9103
-Target /homes/users/U63748/gallopavo.fa
-Chromosome (-) 28
-Program blast
-Query name BLAST_QUERY_MASTER
-Query range 7-144 length:397 coverage: 0.35
-Profile range 7-144 length:411 coverage: 0.34 sec_position: [395]
-Average sequence identity with profile: 0.4103 (ignoring gaps: 0.4669)
-State kept
------- alignment -------
Query VSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLLVVFNFLLMAYFDP <---Intron---> DFYASAPGHKHVPDWVW
/| || ///|| / / | /| /| /| | |/|||||| | |/ /| ||/ |/ | < 2446bp > | | /
Target LSKHQLKRLEEHKYQSAGRSLLEP-LMQGYWEWLVGRVPAWIAPNLITIIGLLINIFTTLLLVYYCP ------------PPWAY
ttacccactggcatcaggctccgc-cacgttgttggagcgtagcacaaaagctaaataacctgtttc ------------cctgt
tcaaatagtaaaaaagcggcttac-ttagagagttggtccgtccattcttgtttattccttttaagc ------------ccgca
gagtggagaaacatgttagggtat-agatcgagataatacgtcatgccctagaatatacgaaaccca ------------tcgac
Query IVVGILNFIAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIF
| || /|| /||||||||||||||||||||| || | |/| |
Target IACACGLFIYQSLDAIDGKQARRTNSSTPLGELFDHGCDSLS----TGIVFSFF
agtgtgctatctcggaggacgaaaaaaaccggctgcgtgtct----agagtttt
tcgcggtttaactactagaacggcaggcctgattaaggactc----cgtttctt
tttttctcccgtgttatggaaggacccttgaattttcttatc----attctttt
------- positions -------
Exon 1 1112021 1112218
Exon 2 1109410 1109574
--------- SECIS ---------
>SelI.15.unaligned.esecis:loosest.1 chromosome:28 strand:- positions:1107472-1107578 species:"Meleagris gallopavo"
target:/homes/users/U63748/gallopavo.fa distance_from_sec_uga:None distance_from_cds:1831
UGGUUUUAGG GUUUUUU UUGUCACAUUGUA CUGAA GCUCUCAGUACUG AG UGUUUGUAGCUCCCUGUGAG CACUGUGGGAUGC UGAU AAU GGCAGAU UUCCUCUUAU
.......((( (.....( ((((((..((... .(((( (((((((.(((.. .. .....)))((((.....))) )....))))).)) )))) ))) )))))). .)))).....
--------- 3' seq --------
Total sequence length available downstream >= 3000
Sequence until first stop codon:
TTAATTGAAGTATGA
L I E V *
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