SELENOPROTEÍNAS DE Meleagris gallopavo
PROTEIN REPORT FOR Methionine sulfoxidereductase A (MsrA)
| DESCRIPCIÓN | Homólogo con cisteína. 25(-):1932125...1930469 |
| SECISearch | Sin resultados. |
| SELENOPROFILES | Encontrada. Elección: Exonerate. Elemento SECIS no predicho. |
| COMENTARIOS | Elección: El alineamiento de las predicciones de Exonerate y Genewise obtuvieron el mismo score pero por defecto reportamos el alineamiento de genewise. |
1. ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
2. BEST PAIRWISE ALIGNMENT
4. RESULTADOS DEL Selenoprofiles
ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=69, Nseq=18, Len=292
SPP00000078_1.0 MLSASRRALQL--LSSANPVRRMGDSASKVISAEE--AL-PGRTEPIPVTAKHHVSGNRT
SPP00000078_1.0.25.exonerate ---------------------RFGDAASFVLSLGT--AL-PGRAERLHVEATHAVTGNPT
SPP00000078_1.0.25.genewise ----------------------------------------PENPDPHPHAATHAVTGNPT
SPP00000012_1.0 MLSATRRACQLLLLHSLFPVPRMGNSASNIVSPQE--AL-PGRKEQTPVAAKHHVNGNRT
SPP00000012_1.0.25.exonerate VLERTRDGCAVLIVLALRETPQVPTNCSALPSPST--AL-PGRAERLHVEATHAVTGNPT
SPP00000012_1.0.25.genewise PR-------------------------SQIPGRGD--ATPPENPDPHPHAATHAVTGNPT
SPP00000039_1.0 MSLTITSSVT-------------------HPELKDLSTV-RNEQKELNISPVHDVN----
SPP00000039_1.0.25.exonerate ------------------------------------------------------------
SPP00000039_1.0.25.genewise ------------------------------------------------------------
SPP00000049_1.0 MVATEH---A-------------------H-------DQ-AGRLENIPQQPLHQID----
SPP00000049_1.0.25.exonerate ------------------------------------------------------------
SPP00000049_1.0.25.genewise ------------------------------------------------------------
SPP00000050_1.0 MVATEH---A-------------------H-------DQ-AGRLENIPQQPLHQID----
SPP00000050_1.0.25.exonerate ------------------------------------------------------------
SPP00000050_1.0.25.genewise ------------------------------------------------------------
SPP00000084_1.0 M-----------------------------------------------------------
SPP00000084_1.0.25.exonerate ------------------------------------------------------------
SPP00000084_1.0.25.genewise ------------------------------------------------------------
SPP00000078_1.0 VEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGHTRNPTYKEVCSEKTGHAE
SPP00000078_1.0.25.exonerate LPPFPPEMQTAIFGMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEEVRSGQTGHAE
SPP00000078_1.0.25.genewise LPPFPPEMQTAIFGMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEEVRSGQTGHAE
SPP00000012_1.0 VEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAE
SPP00000012_1.0.25.exonerate LPPFPPEMQTAIFGMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEEVRSGQTGHAE
SPP00000012_1.0.25.genewise LPPFPPEMQTAIFGMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEEVRSGQTGHAE
SPP00000039_1.0 -----VTKATATFGMGCFWGAESLYGATRGVLRTTVGYAGGSSDLPTYRKM----GDHTE
SPP00000039_1.0.25.exonerate ---------TAIFGMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEE-----TGHAE
SPP00000039_1.0.25.genewise -------------GMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEEVRSGQTGHAE
SPP00000049_1.0 -----TPFEKATFGMGCFWGCDSLFGATKGVLRTRVGYAGGSTESPAYKKM----GDHTE
SPP00000049_1.0.25.exonerate -------MQTAIFGMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEE-----TGHAE
SPP00000049_1.0.25.genewise -------------GMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEEVRSGQTGHAE
SPP00000050_1.0 -----TPFEKATFGMGCFWGCDSLFGATKGVLRTRVGYAGGSTESPAYKKM----GDHTE
SPP00000050_1.0.25.exonerate -------MQTAIFGMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEE-----TGHAE
SPP00000050_1.0.25.genewise -------------GMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEEVRSGQTGHAE
SPP00000084_1.0 -----AYLERAYFGLQCFWG-ESAWAKLKGVVVTRVGYAGGKQPNPTYKNI----KDHTE
SPP00000084_1.0.25.exonerate -------MQTAIFGMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYE----GQTGHAE
SPP00000084_1.0.25.genewise -------------GMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEEVRSGQTGHAE
*: **** : : ** * **:*** *:*. .*:*
SPP00000078_1.0 VVRVVYRPEHISFEELLKVFWENHDPTQGMRQGNDFGTQYRSAVYPTSAVQMEAALRSKE
SPP00000078_1.0.25.exonerate VVRVVFNPNKISYEELLKAFWENHDPTQGMRQQEDVGTQYRSIILTLNPQQQAAALRSRD
SPP00000078_1.0.25.genewise VVRVVFNPNKISYEELLKAFWENHDPTQGXRQQEDVGTQYRSIILTLNPQQQAAALRSRD
SPP00000012_1.0 VVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKE
SPP00000012_1.0.25.exonerate VVRVVFNPNKISYEELLKAFWENHDPTQGMRQQEDVGTQYRSIILTLNPQQQAAALRSRD
SPP00000012_1.0.25.genewise VVRVVFNPNKISYEELLKAFWENHDPTQGXRQQEDVGTQYRSIILTLNPQQQAAALRSRD
SPP00000039_1.0 VLEIDYDPTVISFKELLDLFWNNHEYG----LTTPIKRQYASLILYHDEEQKQVAHASKL
SPP00000039_1.0.25.exonerate VVRVVFNPNKISYEELLKAFWENHGM----RQQEDVGTQYRSIILTLNPQQQAAALRSRD
SPP00000039_1.0.25.genewise VVRVVFNPNKISYEELLKAFWENHD-----------------------------------
SPP00000049_1.0 VIEIDYDPQTISYNDLLDLFWNNHEYG----LTTRMKRQYMSLILYHNEQQRQIAEASRA
SPP00000049_1.0.25.exonerate VVRVVFNPNKISYEELLKAFWENHGM----RQQEDVGTQYRSIILTLNPQQQAAALRSRD
SPP00000049_1.0.25.genewise VVRVVFNPNKISYEELLKAFWENHD-----------------------------------
SPP00000050_1.0 VIEIDYDPQTISYIDLLDLFWNNHEYG----LTTRMKRQYMSLILYHNEQQRQIAEASRA
SPP00000050_1.0.25.exonerate VVRVVFNPNKISYEELLKAFWENHGM----RQQEDVGTQYRSIILTLNPQQQAAALRSRD
SPP00000050_1.0.25.genewise VVRVVFNPNKISYEELLKAFWENH------------------------------------
SPP00000084_1.0 ITEITFDPKVIEYSKLTNFFWKHHNPAE------RRKKQYQSAILYVNDDQKKVAEETLK
SPP00000084_1.0.25.exonerate VVRVVFNPNKISYEELLKAFWENHDPTQ------GMGRRYRSIILTLNPQQQAAALRSRD
SPP00000084_1.0.25.genewise VVRVVFNPNKISYEELLKAFWENHDPT---------------------------------
: .: : * :.: .* . **::*
SPP00000078_1.0 EYQKVLSKHNFGPITTDIREGQVFYYAEDYHQQYLSKN----------------------
SPP00000078_1.0.25.exonerate VYQQELSQQHRGDITTTIEPAGDFYYAEDHHQQYLHKV----------------------
SPP00000078_1.0.25.genewise VYQQELSQQHRGDITTTIEPAGDFYYAEDHHQQYLHKV----------------------
SPP00000012_1.0 NYQKVLSEHGFGPITTDIREGQTFYYAEDYHQQYLSKN----------------------
SPP00000012_1.0.25.exonerate VYQQELSQQHRGDITTTIEPAGDFYYAEDHHQQYLHKV----------------------
SPP00000012_1.0.25.genewise VYQQELSQQHRGDITTTIEPAGDFYYAEDHHQQYLHKV----------------------
SPP00000039_1.0 EEQE---RRAPEIITTEIASKENFYPAEAYHQKYRLQGHKDLASSLNLSPKLLQTSYVAT
SPP00000039_1.0.25.exonerate VYQ-----QHRGDITTTIEPAGDFYYAEDHHQQY--------------------------
SPP00000039_1.0.25.genewise ------------------------------------------------------------
SPP00000049_1.0 EEQV---KRAPEQIITEIAPAGPFYPAENYHQKYRLQGHTDLAKGIGLTPDLLHTSHVAA
SPP00000049_1.0.25.exonerate VYQ-----QHRGDITTTIEPAGDFYYAEDHHQQY--------------------------
SPP00000049_1.0.25.genewise ------------------------------------------------------------
SPP00000050_1.0 EEQV---KRAPEQIITEIAPAGPFYPAENYHQKYRLQGHTDLAKGIGLTPDLLHTSHVAA
SPP00000050_1.0.25.exonerate VYQ-----QHRGDITTTIEPAGDFYYAEDHHQQY--------------------------
SPP00000050_1.0.25.genewise ------------------------------------------------------------
SPP00000084_1.0 VAK-----DKHGDIETYIEPLDKFYQAEDYHQKYWFRQKKILFDELSLLDTQVAEGELAT
SPP00000084_1.0.25.exonerate VYQ-----QHRGDITTTIEPAGDFYYAEDHHQQY--------------------------
SPP00000084_1.0.25.genewise ------------------------------------------------------------
SPP00000078_1.0 -PDGYC-GL------------------------------GGTGVSCPMAIKK
SPP00000078_1.0.25.exonerate -PGGYC-GL------------------------------KGTGVPCPI---A
SPP00000078_1.0.25.genewise -PGGYC-GL------------------------------KGTGVPCPI---A
SPP00000012_1.0 -PNGYC-GL------------------------------GGTGVSCPVGIKK
SPP00000012_1.0.25.exonerate -PGGYC-GL------------------------------KGTGVPCP----I
SPP00000012_1.0.25.genewise -PGGYC-GL------------------------------KGTGVPCP----I
SPP00000039_1.0 KLNGYLAGVGGIEQFKAEAETMGLTPTQRQYCYYHVEQNEGQGLY------C
SPP00000039_1.0.25.exonerate ----------------------------------------------------
SPP00000039_1.0.25.genewise ----------------------------------------------------
SPP00000049_1.0 RLNGYLIGVSGLKQFEDEADLLGLSKDQVQYVREYVIQNEGGGIF------C
SPP00000049_1.0.25.exonerate ----------------------------------------------------
SPP00000049_1.0.25.genewise ----------------------------------------------------
SPP00000050_1.0 RLNGYLIGVSGLKQFEDEADLLGLSKDQVQYVREYVIQNEGGGIF------C
SPP00000050_1.0.25.exonerate ----------------------------------------------------
SPP00000050_1.0.25.genewise ---------------------------------------------------D
SPP00000084_1.0 KLNAYCAGFQDFHDLERLAKEYGLKDSFVNKVKDYALS-GGDPRNC----HA
SPP00000084_1.0.25.exonerate ----------------------------------------------------
SPP00000084_1.0.25.genewise ---------------------------------------------------Q
......................................................................................................................................................................................................................................................
BEST PAIRWISE ALIGNMENT
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=99, Nseq=2, Len=243
SPP00000012_1.0 MLSATRRACQLLLLHSLFPVPRMGNSASNIVSPQEALPGRKEQTP--------VAAKHHV
SPP00000012_1.0.25.genewise --------------------------------PRSQIPGRGDATPPENPDPHPHAATHAV
*:. :*** : ** **.* *
SPP00000012_1.0 NGNRTVEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEK
SPP00000012_1.0.25.genewise TGNPTLPPFPPEMQTAIFGMGCFWGVERLFWEMPGVFSTQVGYAGGFTPNPTYEEVRSGQ
.** *: *** * *:********.** ** : **:*****:***:*.****:** * :
SPP00000012_1.0 TGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAA
SPP00000012_1.0.25.genewise TGHAEVVRVVFNPNKISYEELLKAFWENHDPTQGXRQQEDVGTQYRSIILTLNPQQQAAA
**********::*:::*:*****.********** ** :* ****** * . ..:* **
SPP00000012_1.0 LSSKENYQKVLSEHGFGPITTDIREGQTFYYAEDYHQQYLSKNPNGYCGLGGTGVSCPVG
SPP00000012_1.0.25.genewise LRSRDVYQQELSQQHRGDITTTIEPAGDFYYAEDHHQQYLHKVPGGYCGLKGTGVPCPI-
* *:: **: **:: * *** *. . ******:***** * *.***** ****.**:
SPP00000012_1.0 IKK
SPP00000012_1.0.25.genewise ---
......................................................................................................................................................................................................................................................
RESULTADOS DEL Selenoprofiles
Output_id: MsrA.10.cysteine
---------- ----------------
-Species Meleagris gallopavo -Taxid 9103
-Target /homes/users/U63748/gallopavo.fa
-Chromosome (-) 25
-Program exonerate
-Query name BLAST_QUERY_MASTER
-Query range 10-212 length:218 coverage: 0.93
-Profile range 134-364 length:398 coverage: 0.58 sec_position: [188]
-Average sequence identity with profile: 0.5844 (ignoring gaps: 0.5895)
-State kept
------- alignment -------
Query ALPTPEEALPGRAEPMPVAE <---Intron---> THFVNGHPLKGPFPEGMETAVFG <---Intron---> MGCFWGAERKFWQLPGVYSTAVGYA
|||/| ||||||| / | < 104bp > || | |/| ||| |/||/|| < 89bp > || ||| || ||//|||/|| ||||
Target ALPSPSTALPGRAERLHVEA THAVTGNPTLPPFPPEMQTAIFG MGCFWGVERLFWEMPGVFSTQVGYA
gccacaagccgaggcccggg acggagacaccctccgacagatg agtttgggccttgacggttacggtg
ctcgcgcctcggcagtata ccactcgacctcctccatacctt gtggtggtagttgatcgttccatgac
tcccccgtgacaagggcgg ccccccgtcggtgcctgggggtt cgtccgagggccgggggcctcggatg
*
Query GGYTPNPTYEEVCSG <---Intron---> RTGHTEVVLVVFDPAVISYEQLLKVFWESHDPTQ <---Intron---> GMRQGNDVGTQYRSAIYTT
||/||||||||| || < 181bp > /||| ||| |||/| ||||/||| |||/||||| < 708bp > |||| |||||||| | |
Target GGFTPNPTYEEVRSG QTGHAEVVRVVFNPNKISYEELLKAFWENHDPTQ GMRQQEDVGTQYRSIILTL
ggtacacatgggctg cagcggggcggtacaaaatggccagttgacgcac gacccggggactctaacac
ggtccaccaaatgc gacgacattgtttacaatgaaattactgaaaacca gtgaaaatgcaagctttct
gcccctcctgagca ggggctggggggccccgcctgagggtcggccccag ggggggcgccgccccccag
Query SDEQLAAAEASREAYQA <---Intron---> ALTAAGYGPITTEILPAPPFYYAEDYHQQYLAKNPNGYCGLGGTGVSCPI
/ /| ||| ||/ || < 246bp > |/ | ||| | || ||||||/||||| | | ||||| |||| |||
Target NPQQQAAALRSRDVYQQ ELSQQHRGDITTTIEPAGDFYYAEDHHQQYLHKVPGGYCGLKGTGVPCPI
accccgggccaaggtcc gcaccccggaaaaagcgggtttgggcccctccagcggttgcagaggctca
acaaaccctgggataaa atgaaaggatccctaccgataacaaaaaaataatcggaggtagcgtcgct
ccggggcgcccgttcgg ggcggcagccccccgaatcccccgcccggcgcagcggcccggccgccctt
------- positions -------
Exon 1 1932348 1932405
Exon 2 1932175 1932243
Exon 3 1931966 1932085
Exon 4 1931681 1931784
Exon 5 1930865 1930972
Exon 6 1930469 1930618
--------- SECIS ---------
None found
--------- 3' seq --------
Total sequence length available downstream >= 3000
Sequence until first stop codon:
GCTCCCTGA
A P *
......................................................................................................................................................................................................................................................