SELENOPROTEÍNAS DE Meleagris gallopavo
PROTEIN REPORT FOR Methionine-R-sufoxide reductase 1 (SelR1)
| DESCRIPCIÓN | Selenoproteína. Región: 16(-):6421974...6421606 |
| SECISearch | Elemento predicho en la cadena complementaria. 16:subseq(6411974,10000):[8578,8485] |
| SELENOPROFILES | Encontrada. Elección: Genewise. Predicción elemento SECIS (strand:- positions:6420458-6420553) |
| COMENTARIOS | Elección: Exonerate. Las tres herramientas de análisis apoyan la presencia de esta selenoproteína en el cromosoma 16 de Meleagris gallopavo. Cabe destacar que sólo presenta homología con la SelR1 de Homo sapiens. Predicción coincidente (Sel R1) con la anotación del gen en NCBI. |
1. ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
2. BEST PAIRWISE ALIGNMENT
3. RESULTADOS DEL SECISearch
4. RESULTADOS DEL Selenoprofiles
ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=99, Nseq=3, Len=116
SPP00000021_1.0 MSFCSFFGGEVFQNHFEPGVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPE
SPP00000021_1.0.16.genewise MSFCSFFRGEVFKDHFEPGVYVCARCGYELFSSRAKYEHSSPWPAFTETIHEDSVSKRKE
SPP00000021_1.0.16.exonerate MSFCSFFRGEVFKDHFEPGVYVCARCGYELFSSRAKYEHSSPWPAFTETIHEDSVSKRKE
******* ****::**********:*********:** ************* ***:** *
SPP00000021_1.0 HNRSEALKVSCGKCGNGLGHEFLNDGPKPGQSRFUIFSSSLKFVPKGKETSASQGH
SPP00000021_1.0.16.genewise --RPGALKVSCGKCGNGLGHEFLNDGPKRGQSRFUIFSSSLKFIPKG--------E
SPP00000021_1.0.16.exonerate --RPGALKVSCGKCGNGLGHEFLNDGPKRGQSRFUIFSSSLKFIPKGTSTKNSFGH
*. *********************** ***** ********:*** .
......................................................................................................................................................................................................................................................
BEST PAIRWISE ALIGNMENT
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=99, Nseq=2, Len=116
SPP00000021_1.0 MSFCSFFGGEVFQNHFEPGVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPE
SPP00000021_1.0.16.exonerate MSFCSFFRGEVFKDHFEPGVYVCARCGYELFSSRAKYEHSSPWPAFTETIHEDSVSKRKE
******* ****::**********:*********:** ************* ***:** *
SPP00000021_1.0 HNRSEALKVSCGKCGNGLGHEFLNDGPKPGQSRFUIFSSSLKFVPKGKETSASQGH
SPP00000021_1.0.16.exonerate --RPGALKVSCGKCGNGLGHEFLNDGPKRGQSRFUIFSSSLKFIPKGTSTKNSFGH
*. *********************** ***** ********:***..*. * **
......................................................................................................................................................................................................................................................
RESULTADOS DEL SECISearch
>16:subseq(6411974,10000):[8578,8485] [8485 - 8578] (SECIS on complementary strand) - Free Energy: -34.03 UCUGGUGCUAUUCUGGGCUGGGAUCAUGGUAUGAAUUGUCCUCUGUAAAUCCUCUUGGGAUGUGAGGGCAGUGAUGUUUUAUGAUCCUGCUGGA
_[8578,8485].png)
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RESULTADOS DEL Selenoprofiles
Output_id: SelR.1.selenocysteine
---------- ---------------------
-Species Meleagris gallopavo -Taxid 9103
-Target /homes/users/U63748/gallopavo.fa
-Chromosome (-) 16
-Program genewise
-Query name gi|182676385|sp|Q802G6.2|MSRB1_DANRE RecName: Full=Methionine-R-sulfoxide reductase B1_
AltName: Full=Selenoprotein X_ Short=SelX gi|29648559|gb|AAO86699.1| methionine R-sulfoxide reductase [Danio rerio]
-Query range 1-104 length:110 coverage: 0.95
-Profile range 1-512 length:792 coverage: 0.65 sec_position: [448]
-Average sequence identity with profile: 0.2607 (ignoring gaps: 0.3808)
-State kept
------- alignment -------
Query MSFCSFSGGEIYKDHFESG <---Intron---> MYVCAQCGYELFSSRSKYEHSSPWPAFTETIHKDSVSKQEERWGAYK <---Intron---> VR
|||||| ||//||||| | < 215bp > /||||/|||||||||/||||||||||||||||/|||||//|| || | < 102bp > |
Target MSFCSFFRGEVFKDHFEPG VYVCARCGYELFSSRAKYEHSSPWPAFTETIHEDSVSKRKERPGALK VS
attttttcgggtagctgcg gtgtgctgtgcttacgatgcttctcgtagaacggagtacagccggta gt
tctgcttggattaaatac gtatgcgggaattcggcaaaacccgcctcactaaagtcagaagcgcta tc
ggccgttgcagcgcccgg tgtaccgcctggccctcacgcaccggcccgcccgccgtgcggtggcgg gc
Query CGKCGNGLGHEFVNDGPKHGLSRFUIFSSSLKFIPK
||||||||||||/||||| | |||||||||||||||
Target CGKCGNGLGHEFLNDGPKRGQSRFUIFSSSLKFIPK
tgatgagcgcgtcaggcacgctcttataatcataca
ggaggagtgaattaagcaggacgtgttggctattca
tcgtctggccgccccccggggcgcaacccgcaccta
*
------- positions -------
Exon 1 6422186 6422240
Exon 2 6421828 6421970
Exon 3 6421612 6421725
--------- SECIS ---------
>SelR.1.selenocysteine.esecis:str.1 chromosome:16 strand:- positions:6420458-6420553 species:"Meleagris gallopavo"
target:/homes/users/U63748/gallopavo.fa distance_from_sec_uga:1091 distance_from_cds:1058
CUCUGGUGCU AUUCUGGG CUGGGAUCAUGGU AUGAA UUGUCCUCUGU AA AUCCUCUUGGGAU GUGAGGGCAG UGAU GUU UUAUGAU CCUGCUGGAU
.((((((((( ......)) ).((((((((((. .(((( ((((((((... .. (((((...))))) ..)))))))) )))) ... ))))))) ))))))))).
--------- 3' seq --------
Total sequence length available downstream >= 3000
Sequence until first stop codon:
GGTGAGGGATGGGGTTTGGTGGCTGCCTCCATCCGTGCTGTTATTCTGGGGGGAGCCACTAAAGCCTGGCCATGCTGTGAGGGGGTGTGCTGCTCCCAGTCTTAA
G E G W G L V A A S I R A V I L G G A T K A W P C C E G V C C S Q S *
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