SELENOPROTEÍNAS DE Meleagris gallopavo
PROTEIN REPORT FOR Iodothyronine deiodinase 2 (DI 2)
| DESCRIPCIÓN | Selenoproteína. 5(-):42327820...42315802. |
| SECISearch | Sin resultados. |
| SELENOPROFILES | Encontrada. Elección: Genewise. No se han predicho elementos SECIS. |
| COMENTARIOS | Elección: Genewise. Ausencia de SECIS (que no descarta el hecho de que sea una selenoproteína). La homología con la DI de otros organismos es clara viendo el multiple-alignment. Con el pairwise-aligment se obtuvo un mayor SCORE para la DI 1, coincidente con la anotación de NCBI. |
1. ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
2. BEST PAIRWISE ALIGNMENT
4. RESULTADOS DEL Selenoprofiles
ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=66, Nseq=15, Len=311
SPP00000002_1.0 MGILSVDLLIT----------LQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGE
SPP00000002_1.0.5.exonerate MGLLSVDLLIT----------LQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGE
SPP00000002_1.0.5.genewise MGLLSVDLLIT----------LQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGE
SPP00000097_1.0 MGLLSVDLLIT----------LQILPVFFSNCLFLALYDSVILLKHVALLLSRSKSTRGE
SPP00000097_1.0.5.exonerate MGLLSVDLLIT----------LQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGE
SPP00000097_1.0.5.genewise MGLLSVDLLIT----------LQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGE
SPP00000001_1.0 MGLPQPGLW------------LKRLWVLLEVAVHVVVGKVL-----LILFPDRVKRN---
SPP00000001_1.0.5.exonerate ----------N----------NGKAPFYFTE-----------------------------
SPP00000001_1.0.5.genewise ------------------------------------------------------------
SPP00000003_1.0 ML--RSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKH---FLGRRRRGQPE
SPP00000003_1.0.5.exonerate ------------------------------------------------------------
SPP00000003_1.0.5.genewise ------------------------------------------------------------
SPP00000096_1.0 MGLPQLWLW------------LKRLVIFLQVALEVAVGKVL-----MTLFPGRVKQS---
SPP00000096_1.0.5.exonerate ------------------------------------------------------------
SPP00000096_1.0.5.genewise ------------------------------------------------------------
SPP00000002_1.0 WRRMLT-----------------------SEGLRCVWKSFLL---DAYKQVKLGEDAPNS
SPP00000002_1.0.5.exonerate WRRMLT-----------------------SEGLRCVWNSFLL---DAYKQVKLGGEAPNS
SPP00000002_1.0.5.genewise WRRMLT-----------------------SEGLRCVWNSFLL---DAYKQVKLGGEAPNS
SPP00000097_1.0 WRRMLT-----------------------SEGLRCVWNSFLL---DAYKQVKLGEDAPNS
SPP00000097_1.0.5.exonerate WRRMLT-----------------------SEGLRCVWNSFLL---DAYKQVKLGGEAPNS
SPP00000097_1.0.5.genewise WRRMLT-----------------------SEGLRCVWNSFLL---DAYKQVKLGGEAPNS
SPP00000001_1.0 ----ILAMGEKTGMTRNPH-FSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNC
SPP00000001_1.0.5.exonerate ----LW-----------------------ATKLEGYWKTL-------IDTVKLGGEAPNS
SPP00000001_1.0.5.genewise ------------------------------------------------------------
SPP00000003_1.0 PEVELNSEGEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKL---DFFKQAHEGGPAPNS
SPP00000003_1.0.5.exonerate -RRMLT-----------------------SEGLRCVWNSFLL---DAYKQVKLGGEAPNS
SPP00000003_1.0.5.genewise ------------------------------------------------------------
SPP00000096_1.0 ----ILAMGQKTGMARNPR-FAPDNWVPTFFSIQYFWFVLKVRWQRLEDRAEFGGLAPNC
SPP00000096_1.0.5.exonerate ------------------------------------------------------------
SPP00000096_1.0.5.genewise ------------------------------------------------------------
SPP00000002_1.0 SVVHVSSTEGGDNSGNGT-QEKIAEG-ATCHLLDFASPERPLVVNFGSATUPPFTSQLPA
SPP00000002_1.0.5.exonerate SVIHIANG--NDGSGSSWKSVGGKCG-TKCHLLDFANSERPLVVNFGSATUPPFTSQLSA
SPP00000002_1.0.5.genewise SVIHIANG--NDGSGSSWKSVGGKCG-TKCHLLDFANSERPLVVNFGSATUPPFTSQLSA
SPP00000097_1.0 SVVHVSNPE----SGNNYASEKTADG-AECHLLDFASAERPLVVNFGSAT--PFTRQLPA
SPP00000097_1.0.5.exonerate SVIHIANG--NDGSGSSWKSVGGKCG-TKCHLLDFANSERPLVVNFGSATUPPFTSQLSA
SPP00000097_1.0.5.genewise SVIHIANG--NDGSGSSWKSVGGKCG-TKCHLLDFANSERPLVVNFGSATUPPFTSQLSA
SPP00000001_1.0 PVVRL-------------------SG-QRCNIWEFMQGNRPLVLNFGSCTUPSFMFKFDQ
SPP00000001_1.0.5.exonerate SVIHI-------------------CG-TKCHLLDFANSERPLVVNFGSATUPPFTSQLSA
SPP00000001_1.0.5.genewise -------------------------G-TKCHLLDFANSERPLVVNFGSATUPPFTSQLSA
SPP00000003_1.0 EVVLP-------------------DGFQSQHILDYAQGNRPLVLNFGSCTUPPFMARMSA
SPP00000003_1.0.5.exonerate SSV------------------GGKCG-TKCHLLDFANSERPLVVNFGSATUPPFTSQLSA
SPP00000003_1.0.5.genewise ------------------------------HLLDFANSERPLVVNFGSATUPPFTSQLSA
SPP00000096_1.0 TVVCL-------------------SG-QKCNIWDFIQGSRPLVLNFGSCTUPSFLLKFDQ
SPP00000096_1.0.5.exonerate -------------------------G-TKCHLLDFANSERPLVVNFGSATUPPFTSQLSA
SPP00000096_1.0.5.genewise -------------------------G-TKCHLLDFANSERPLVVNFGSATUPPFTSQLSA
:: :: . .****:****.* .* ::
SPP00000002_1.0 FRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERF
SPP00000002_1.0.5.exonerate FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
SPP00000002_1.0.5.genewise FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
SPP00000097_1.0 FRQLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSLSFEVKKHRNQEDRCAAAHQLLERF
SPP00000097_1.0.5.exonerate FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
SPP00000097_1.0.5.genewise FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
SPP00000001_1.0 FKRLIEDFSSIADFLVIYIEEAHASDGWAFK----NNMDIRNHQNLQDRLQAAHLLLAR-
SPP00000001_1.0.5.exonerate FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
SPP00000001_1.0.5.genewise FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
SPP00000003_1.0 FQRLVTKYQRDVDFLIIYIEEAHPSDGWVT---TDSPYIIPQHRSLEDRVSAARVLQQG-
SPP00000003_1.0.5.exonerate FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
SPP00000003_1.0.5.genewise FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
SPP00000096_1.0 FKRLV--FASTADFLIIYIEEAHATDGWAFK----NNVDIRQHRSLQERVRAARMLLAR-
SPP00000096_1.0.5.exonerate FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
SPP00000096_1.0.5.genewise FSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAYQLLERF
* :*: : .***::**:***.:***. : :*:. ::* ** *
SPP00000002_1.0 SLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEKNF
SPP00000002_1.0.5.exonerate SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNF
SPP00000002_1.0.5.genewise SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNF
SPP00000097_1.0 SLPPQCQVVADRMDNNANVAYGVAFERVCIVQRRKIAYLGGKGPFSYNLQEVRSWLEKNF
SPP00000097_1.0.5.exonerate SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNF
SPP00000097_1.0.5.genewise SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNF
SPP00000001_1.0 --SPQCPVVVDTMQNQSSQLYAALPERLYIIQEGRILYKGKSGPWNYNPEEVRAVLEKLH
SPP00000001_1.0.5.exonerate SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLE---
SPP00000001_1.0.5.genewise SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLE---
SPP00000003_1.0 --APGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYD
SPP00000003_1.0.5.exonerate SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLE---
SPP00000003_1.0.5.genewise SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLE---
SPP00000096_1.0 --SPQCPVVVDTMQNQSSQLYAALPERLYVIQEGRICYKGKAGPWNYNPEEVRAVLEKLC
SPP00000096_1.0.5.exonerate SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLE---
SPP00000096_1.0.5.genewise SLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLE---
.* * :* * * *.:. *.. **: ::* * * * ** *: .*:* **
SPP00000002_1.0 SK--------R
SPP00000002_1.0.5.exonerate SK--------R
SPP00000002_1.0.5.genewise SK--------R
SPP00000097_1.0 SKR-----ILD
SPP00000097_1.0.5.exonerate SK--------R
SPP00000097_1.0.5.genewise SK--------R
SPP00000001_1.0 ----------S
SPP00000001_1.0.5.exonerate ----------Q
SPP00000001_1.0.5.genewise ----------Q
SPP00000003_1.0 EQLHGARPRRV
SPP00000003_1.0.5.exonerate ----------Q
SPP00000003_1.0.5.genewise ----------Q
SPP00000096_1.0 TPP--RHVPQL
SPP00000096_1.0.5.exonerate ----------Q
SPP00000096_1.0.5.genewise ----------Q
......................................................................................................................................................................................................................................................
BEST PAIRWISE ALIGNMENT
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=98, Nseq=2, Len=267
SPP00000097_1.0 MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRSKSTRGEWRRMLTSEGL
SPP00000097_1.0.5.genewise MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGEWRRMLTSEGL
************************************:.*:******:*************
SPP00000097_1.0 RCVWNSFLLDAYKQVKLGEDAPNSSVVHVSNPE--SGNNYASEKTADGAECHLLDFASAE
SPP00000097_1.0.5.genewise RCVWNSFLLDAYKQVKLGGEAPNSSVIHIANGNDGSGSSWKSVGGKCGTKCHLLDFANSE
****************** :******:*::* : **..: * *::*******.:*
SPP00000097_1.0 RPLVVNFGSAT--PFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSLSFE
SPP00000097_1.0.5.genewise RPLVVNFGSATUPPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFE
*********** *** **.** :******.*******************.** *. ***
SPP00000097_1.0 VKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQRRKIAYL
SPP00000097_1.0.5.genewise VKKHRNQEDRCAAAYQLLERFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYL
**************:***************** ***********:*********:*****
SPP00000097_1.0 GGKGPFSYNLQEVRSWLEKNFSKRILD
SPP00000097_1.0.5.genewise GGKGPFFYNLQEVRLWLEQNFSKR---
****** ******* ***:*****
......................................................................................................................................................................................................................................................
RESULTADOS DEL Selenoprofiles
Output_id: DI.1.selenocysteine
---------- -------------------
-Species Meleagris gallopavo -Taxid 9103
-Target /homes/users/U63748/gallopavo.fa
-Chromosome (-) 5
-Program genewise
-Query name gi|156547239|ref|NP_989445.2| deiodinase, iodothyronine, type II [Gallus gallus]
-Query range 1-264 length:264 coverage: 1.0
-Profile range 1-464 length:486 coverage: 0.95 sec_position: [257]
-Average sequence identity with profile: 0.3288 (ignoring gaps: 0.4283)
-State kept
------- alignment -------
Query MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGEWRRMLTSEGLRCVWNSFLLDAYKQ <---Intron---> VKLGGEAPNS
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| < 11227bp > ||||||||||
Target MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGEWRRMLTSEGLRCVWNSFLLDAYKQ VKLGGEAPNS
agctaggtcaaccatcgtttatctcgctgtgaccacagctcactatgcggtcaacatggcctgtaatccggtac gacggggcat
tgttgtatttctattctttcagtttctaacttttaatttttggcaccggagggttccagtggtgagtttacaaa tatggaccac
gtgatgtggcgtgcggctcctccctggtctgccggcgggtgcctgtgctggggggcggggcccgccccgcccgg cataaatacc
Query SVIHIAKGNDGSNSSWKSVGGKCGTKCHLLDFANSERPLVVNFGSATUPPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKH
|||||| ||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Target SVIHIANGNDGSGSSWKSVGGKCGTKCHLLDFANSERPLVVNFGSATUPPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKH
agacagagaggagaataagggatgaatcccgtgatgcccggatgtgatcctaacctgtaacgggttggggtctgtagggcctggtggcgatctttggaac
gttatcagaaggggggagtggaggcagattatcacagctttatgcccgcctcgatcctgattaatcgtcattttataacaccaggcccgtcccctataaa
taccactctccctccggttttgtgcgcttgttcctggagtttccattaagcacggtcccgggagcttgtctggccctgtcaatcgttttccctctatggc
*
Query RNQEDRCAAAHQLLERFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNFSKR
||||||||||/|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Target RNQEDRCAAAYQLLERFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNFSKR
aacggctgggtcccgctttccctcggggtagaagaggtggttgcgtagcacaagtcggagctttaccggccttgcataaa
gaaaaggcccaattagtctccagattcagtaaacatcagtctagtgttagaatcatggagcttaataatgtgtaaatgag
gtgatatattcacgacccgatgtaggtccgcctttgctttatgaactggaaacccgatacctccttggtttgggacccaa
------- positions -------
Exon 1 42327599 42327820
Exon 2 42315802 42316371
--------- SECIS ---------
None found
--------- 3' seq --------
Total sequence length available downstream >= 3000
Sequence until first stop codon:
TGA
*
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