Command line: [exonerate -m p2g --showtargetgff -q SPP00000009_1.0.fa -t SPP00000009_1.0.GL397305.1/GL397305.1.subseq] Hostname: [upf-OptiPlex-755] C4 Alignment: ------------ Query: SPP00000009_1.0 # Protein # Glutathione peroxidase 6 (GPx6) # Homo sapiens # Complete Target: GL397305.1:subseq(18711209,102757) dna:supercontig supercontig:Nleu1.0:GL397305.1:1:19291937:1 REF Model: protein2genome:local Raw score: 804 Query range: 0 -> 217 Target range: 43956 -> 52757 1 : MetPheGlnGlnPheGlnAlaSerCysLeuValLeuPhePheLeuValGlyPheAlaGln : 20 ||| !!:!! !.!.|||||||||||||||:!!! !.!..!!|||!.!||||||!.!! ! MetAlaArgLeuLeuGlnAlaSerCysLeuLeuSerLeuLeuLeuAlaGlyPheValPro 43957 : ATGGCCCGGCTGCTGCAGGCGTCCTGCCTGCTCTCCCTGCTCCTGGCCGGCTTCGTCCCG : 44014 21 : GlnThrLeuLysProGlnAsnArgLys >>>> Target Intron 1 >>>> ValA : 31 |||!:!! ! !! !:!!!!. !||| 5959 bp :!!| GlnSerArgGlyGlnGluLysSerLys++ ++MetA 44015 : CAGAGCCGGGGACAAGAGAAGTCGAAGgt.........................agATGG : 50006 32 : spCysAsnLysGlyValThrGlyThrIleTyrGluTyrGlyAlaLeuThrLeuAsnGlyG : 51 |||||:!! |||:!!!:!.!!|||||||||||||||||||||||||||:!!:!!|||| spCysHisGlyGlyMetSerSerThrIleTyrGluTyrGlyAlaLeuThrIleAspGlyG 50007 : ACTGCCATGGTGGCATGAGCAGCACCATTTATGAGTACGGAGCCCTCACCATTGATGGGG : 50066 52 : luGluTyrIleGlnPheLysGlnPheAlaGlyLysHisValLeuPheValAsnValAlaA : 71 |||||||||||! |||||||||!:!|||||||||:!!|||||||||||||||||||||: luGluTyrIleProPheLysGlnTyrAlaGlyLysTyrValLeuPheValAsnValAlaS 50067 : AGGAGTACATCCCCTTCAAGCAGTATGCTGGCAAATACGTCCTCTTTGTCAACGTGGCCA : 50126 72 : laTyrUnkGlyLeuAlaAlaGlnTyrPro{G} >>>> Target Intron 2 >>>> : 81 :!||| ||||||.!!!.!|||||| !{|} 1390 bp erTyr***GlyLeuThrGlyGlnTyrIle{G}++ + 50127 : GCTACTGAGGCCTGACAGGCCAGTACATT{G}gt.........................a : 51545 82 : {lu}LeuAsnAlaLeuGlnGluGluLeuLysAsnPheGlyValIleValLeuAlaPheP : 100 {||}|||||||||||||||||||||||| ! ||||||:!!:!!:!!|||!.!|||| +{lu}LeuAsnAlaLeuGlnGluGluLeuAlaProPheGlyLeuValIleLeuGlyPheP 51546 : g{AA}CTGAATGCACTACAGGAAGAGCTTGCACCATTTGGTCTGGTCATTCTGGGCTTTC : 51603 101 : roCysAsnGlnPheGlyLysGlnGluProGlyThrAsnSerGluIleLeuLeuGlyLeu{ : 120 |||||||||||||||||||||||||||||||| !|||||||||||||||! ! !|||{ roCysAsnGlnPheGlyLysGlnGluProGlyGluAsnSerGluIleLeuProThrLeu{ 51604 : CCTGCAACCAGTTTGGAAAACAGGAACCAGGAGAGAACTCAGAGATCCTTCCCACCCTC{ : 51663 121 : Ly} >>>> Target Intron 3 >>>> {s}TyrValCysProGlySerGlyPheV : 129 ||} 415 bp {|}|||||| ! ||||||.!!||||||| Ly}++ ++{s}TyrValArgProGlyGlyGlyPheV 51664 : AA}gt.........................ag{G}TATGTCCGACCAGGTGGAGGCTTTG : 52105 130 : alProSerPheGlnLeuPheGluLysGlyAspValAsnGlyGluLysGluGlnLysValP : 149 |||||!:!|||||||||||||||||||||||||||||||||||||||||||||||| !!! alProAsnPheGlnLeuPheGluLysGlyAspValAsnGlyGluLysGluGlnLysPheT 52106 : TCCCTAATTTCCAGCTCTTTGAGAAAGGGGATGTCAATGGAGAGAAAGAGCAGAAATTCT : 52165 150 : heThrPheLeuLys >>>> Target Intron 4 >>>> AsnSerCysProProTh : 159 :!|||||||||||| 386 bp ||||||||||||||||| yrThrPheLeuLys++ ++AsnSerCysProProTh 52166 : ACACTTTCCTAAAGgt.........................agAACTCCTGTCCTCCCAC : 52581 160 : rSerAspLeuLeuGlySerSerSerGlnLeuPheTrpGluProMetLysValHisAspIl : 179 ||||!!:|||||||||!!!|||..!!::|||||||||||||||||||||||||||||||| rSerGluLeuLeuGlyThrSerAspArgLeuPheTrpGluProMetLysValHisAspIl 52582 : CTCGGAGCTCCTGGGTACATCTGACCGCCTCTTCTGGGAACCCATGAAGGTTCACGACAT : 52641 180 : eArgTrpAsnPheGluLysPheLeuValGlyProAspGlyValProValMetHisTrpPh : 199 ||||||||||||||||||||||||||||||||||||||||..!|||:!!|||!.!||| eArgTrpAsnPheGluLysPheLeuValGlyProAspGlyThrProIleMetArgTrpHi 52642 : CCGCTGGAACTTTGAGAAGTTCCTGGTGGGGCCAGATGGTACACCCATCATGCGCTGGCA : 52701 200 : eHisGlnAlaProValSerThrValLysSerAspIleLeuGluTyrLeuLysGln : 217 !|||!:!.!! !!||||||!.!||||||! !|||||||||...|||:!!!:!!:! sHisArgThrThrValSerAsnValLysMetAspIleLeuSerTyrMetArgArg 52702 : CCACCGGACCACGGTCAGCAACGTCAAGATGGACATCCTGTCCTACATGAGGCGG : 52757 vulgar: SPP00000009_1.0 0 217 . GL397305.1:subseq(18711209,102757) 43956 52757 + 804 M 29 87 5 0 2 I 0 5955 3 0 2 M 51 153 S 0 1 5 0 2 I 0 1386 3 0 2 S 1 2 M 38 114 S 0 2 5 0 2 I 0 411 3 0 2 S 1 1 M 33 99 5 0 2 I 0 382 3 0 2 M 64 192 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2012-11-29 ##type DNA # # # seqname source feature start end score strand frame attributes # GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local gene 43957 52757 804 + . gene_id 1 ; sequence SPP00000009_1.0 ; gene_orientation + GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local cds 43957 44043 . + . GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local exon 43957 44043 . + . insertions 0 ; deletions 0 GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local splice5 44044 44045 . + . intron_id 1 ; splice_site "GT" GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local intron 44044 50002 . + . intron_id 1 GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local splice3 50001 50002 . + . intron_id 0 ; splice_site "AG" GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local cds 50003 50156 . + . GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local exon 50003 50156 . + . insertions 0 ; deletions 0 GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local splice5 50157 50158 . + . intron_id 2 ; splice_site "GT" GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local intron 50157 51546 . + . intron_id 2 GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local splice3 51545 51546 . + . intron_id 1 ; splice_site "AG" GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local cds 51547 51664 . + . GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local exon 51547 51664 . + . insertions 0 ; deletions 0 GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local splice5 51665 51666 . + . intron_id 3 ; splice_site "GT" GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local intron 51665 52079 . + . intron_id 3 GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local splice3 52078 52079 . + . intron_id 2 ; splice_site "AG" GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local cds 52080 52179 . + . GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local exon 52080 52179 . + . insertions 0 ; deletions 0 GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local splice5 52180 52181 . + . intron_id 4 ; splice_site "GT" GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local intron 52180 52565 . + . intron_id 4 GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local splice3 52564 52565 . + . intron_id 3 ; splice_site "AG" GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local cds 52566 52757 . + . GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local exon 52566 52757 . + . insertions 0 ; deletions 0 GL397305.1:subseq(18711209,102757) exonerate:protein2genome:local similarity 43957 52757 804 + . alignment_id 1 ; Query SPP00000009_1.0 ; Align 43957 1 87 ; Align 50003 30 153 ; Align 51549 82 114 ; Align 52081 121 99 ; Align 52566 154 192 # --- END OF GFF DUMP --- # -- completed exonerate analysis