TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000097_1.0 # Protein # Iodothyronine deiodinase 2 (DI2) #
Mus musculus # Complete
         (264 letters)

Database: /cursos/BI/genomes/project_2013/Mustela_putorius_furo/genome
.fa 
           4245 sequences; 2,400,181,698 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|JH418057.1| Mustela putorius furo unplaced genomic scaffold s...   332   1e-89
gb|JH418017.1| Mustela putorius furo unplaced genomic scaffold s...   150   7e-35
gb|JH418061.1| Mustela putorius furo unplaced genomic scaffold s...    89   2e-28
gb|JH417975.1| Mustela putorius furo unplaced genomic scaffold s...    56   1e-06
gb|JH418311.1| Mustela putorius furo unplaced genomic scaffold s...    35   3.4  
gb|JH418183.1| Mustela putorius furo unplaced genomic scaffold s...    35   3.4  
gb|JH418114.1| Mustela putorius furo unplaced genomic scaffold s...    34   7.6  
gb|JH417993.1| Mustela putorius furo unplaced genomic scaffold s...    34   7.6  

>gb|JH418057.1| Mustela putorius furo unplaced genomic scaffold scaffold00085, whole genome
               shotgun sequence
          Length = 8401080

 Score =  332 bits (850), Expect = 1e-89
 Identities = 171/219 (78%), Positives = 183/219 (83%), Gaps = 5/219 (2%)
 Frame = +1

Query: 51      WRRMLTSEGLRCVWNSFLLDAYKQVKLGEDAPNSSVVHVSNPESGNNY---ASEKTADGA 107
               +R   + +    V++   L  + QVKLGEDAPNSSVVHV+N E  +N    A  K  DGA
Sbjct: 2565052 YRVKWSDDAFHMVFSPSFLFFHHQVKLGEDAPNSSVVHVANAEGSDNSRNGAQVKMVDGA 2565231

Query: 108     ECHLLDFASAERPLVVNFGSAT--PFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPSDGW 165
               ECHLLDFAS ERPLVVNFGSAT  PFT QLPAF +LVEEFSSVADFLLVYIDEAHPSDGW
Sbjct: 2565232 ECHLLDFASPERPLVVNFGSAT*PPFTSQLPAFSKLVEEFSSVADFLLVYIDEAHPSDGW 2565411

Query: 166     AVPGDSSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVAFERV 225
               AVPG+SSLSFEVK HRNQEDRCAAAHQLLE FSLPPQC+VVADRMDNNANVAYGVAFERV
Sbjct: 2565412 AVPGESSLSFEVKTHRNQEDRCAAAHQLLEHFSLPPQCRVVADRMDNNANVAYGVAFERV 2565591

Query: 226     CIVQRRKIAYLGGKGPFSYNLQEVRSWLEKNFSKRXILD 264
               CIVQR+KIAYLGGKGPF YNLQEVR WLEKNFSKR  LD
Sbjct: 2565592 CIVQRQKIAYLGGKGPFCYNLQEVRRWLEKNFSKR*NLD 2565708



 Score =  144 bits (363), Expect = 4e-33
 Identities = 70/75 (93%), Positives = 73/75 (97%)
 Frame = +1

Query: 1       MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRSKSTRGEWRRMLTSEGL 60
               MG+LSVDLLITLQILPVFFSNCLFLALYDSVILLKHV LLLSRSKSTRGEWRRMLTSEG+
Sbjct: 2556805 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGM 2556984

Query: 61      RCVWNSFLLDAYKQV 75
               RC+W SFLLDAYKQV
Sbjct: 2556985 RCIWKSFLLDAYKQV 2557029


>gb|JH418017.1| Mustela putorius furo unplaced genomic scaffold scaffold00045, whole genome
               shotgun sequence
          Length = 12738967

 Score =  150 bits (378), Expect = 7e-35
 Identities = 88/204 (43%), Positives = 122/204 (59%), Gaps = 2/204 (0%)
 Frame = -2

Query: 53      RMLTSEGLRCVWNSFLLDAYKQVKLGEDAPNSSVVHVSNPESGNNYASEKTADGAECHLL 112
               R+ T   L+ VW+   LD +KQ   G  APNS VV    P    N            H+L
Sbjct: 2613657 RLCTLASLKAVWHGQKLDFFKQAYEGGPAPNSEVVL---PHGFQNQ-----------HIL 2613520

Query: 113     DFASAERPLVVNFGSAT--PFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPSDGWAVPGD 170
               D+A   RPLV+NFGS T  PF  ++ AF +LV ++    DFL++YI+EAHPSDGW V  D
Sbjct: 2613519 DYARGNRPLVLNFGSCT*PPFMARMSAFHRLVTKYQRDVDFLIIYIEEAHPSDGW-VTTD 2613343

Query: 171     SSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQR 230
               S  S  + +HR+ EDR +AA Q+L++ +  P C +V D M N+++ AYG  FER+ ++Q 
Sbjct: 2613342 SPYS--IPQHRSLEDRVSAA-QVLQQGA--PSCSLVLDTMANSSSSAYGAYFERLYVIQN 2613178

Query: 231     RKIAYLGGKGPFSYNLQEVRSWLE 254
                 I Y GG+GP  Y + E+R+WLE
Sbjct: 2613177 GTIMYQGGRGPDGYQVSELRTWLE 2613106


>gb|JH418061.1| Mustela putorius furo unplaced genomic scaffold scaffold00089, whole
              genome shotgun sequence
          Length = 8322713

 Score = 89.4 bits (220), Expect = 2e-16
 Identities = 67/142 (47%), Positives = 81/142 (57%), Gaps = 7/142 (4%)
 Frame = -1

Query: 50     EWRRMLT--SEGLRCVWNSFLLDAYKQVKLGEDAPNS---SVVHVSNPESGNNYASEKTA 104
              EWR MLT   E       S  ++AY+QVKLGE++P         +    +       +  
Sbjct: 256604 EWRPMLTWARECAASGKASSPINAYRQVKLGEESPVPVWCMFPILKEVTTLKMVPM*RWL 256425

Query: 105    DGAECHLLDFASAERPLVVNFGSAT--PFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPS 162
                    LL   S E  LVVNF SAT  PFT  LPAF + VEEFS VADFLLVY+DE H S
Sbjct: 256424 I*LNATLLT-GSPEHLLVVNFDSAT*PPFTS*LPAFSKRVEEFS-VADFLLVYMDETHLS 256251

Query: 163    DGWAVPGDSSLSFEVKKHRNQE 184
              + W+V GDSSLSFEVKKH+  +
Sbjct: 256250 EDWSVSGDSSLSFEVKKHQKTD 256185



 Score = 84.7 bits (208), Expect(3) = 2e-28
 Identities = 73/194 (37%), Positives = 90/194 (46%), Gaps = 28/194 (14%)
 Frame = -3

Query: 58     EGLRCVWNSFLLDAYKQV-KLGEDAPNSSVVHVSNPESGNNY---ASEKTADGAECHLLD 113
              +G+ C+W SF  +      K+G   P+SSVV+VSN E G+N    A  K  D AECHL+D
Sbjct: 256578 QGMCCIW*SFFPNQCL*AGKIG*RIPSSSVVYVSNSEGGDNIKNGAHVKMVDIAECHLID 256399

Query: 114    F---ASAERPL----VVNFGSATPFTRQLP-----------------AFRQLVEEFSSVA 149
              +   A     L    V +F       +Q                   +FR+LV    SV 
Sbjct: 256398 WQP*APTGG*L*LSHVTSFY*LAASLQQTSGRVLSG*LSVGVHG*DSSFRRLV----SVW 256231

Query: 150    DFLLVYIDEAHPSDGWAVPGDSSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADR 209
               FL V+  E  P                      EDRCAA HQLLE F LPPQCQVVA+ 
Sbjct: 256230 *FLFVF*SEETP----------------------EDRCAADHQLLEHFFLPPQCQVVAEH 256117

Query: 210    MDNNANVAYGVAFE 223
              MD+N N  Y VAFE
Sbjct: 256116 MDSNPNAVYDVAFE 256075



 Score = 50.8 bits (120), Expect(3) = 2e-28
 Identities = 24/32 (75%), Positives = 26/32 (81%)
 Frame = -2

Query: 233    IAYLGGKGPFSYNLQEVRSWLEKNFSKRXILD 264
              IAYLGG GPF YNLQE++  LEKNFSKR  LD
Sbjct: 256075 IAYLGGNGPFYYNLQEIQH*LEKNFSKRWNLD 255980



 Score = 33.9 bits (76), Expect(3) = 2e-28
 Identities = 16/29 (55%), Positives = 18/29 (62%)
 Frame = -2

Query: 34     LKHVALLLSRSKSTRGEWRRMLTSEGLRC 62
              L ++ LLLS SKS   EWR MLT  G  C
Sbjct: 256675 LGYIVLLLSHSKSADAEWRPMLTQSGGPC 256589


>gb|JH417975.1| Mustela putorius furo unplaced genomic scaffold scaffold00003, whole genome
               shotgun sequence
          Length = 31471863

 Score = 56.2 bits (134), Expect = 1e-06
 Identities = 27/49 (55%), Positives = 37/49 (75%), Gaps = 2/49 (4%)
 Frame = +2

Query: 116     SAERPLVVNFGSAT--PFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPS 162
               S  RPLV+NFGS T   F  +L  F++L+E+F S+ADFL++YI+EAH S
Sbjct: 4299521 SGNRPLVLNFGSCT*PSFLFKLDQFKRLIEDFCSMADFLIIYIEEAHAS 4299667



 Score = 49.7 bits (117), Expect = 1e-04
 Identities = 28/79 (35%), Positives = 43/79 (54%)
 Frame = +2

Query: 162     SDGWAVPGDSSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVA 221
               +DGWA   + +    ++ HRN +DR  AA  LL+R    PQC VV D M+N ++  Y   
Sbjct: 4303199 ADGWAFKNNVN----IRTHRNLQDRLRAARLLLDR---NPQCPVVVDTMENQSSQRYAAL 4303357

Query: 222     FERVCIVQRRKIAYLGGKG 240
                ER+ ++   +I Y   +G
Sbjct: 4303358 PERLYVLWEGRILYKVTRG 4303414


>gb|JH418311.1| Mustela putorius furo unplaced genomic scaffold scaffold00339, whole
              genome shotgun sequence
          Length = 1139275

 Score = 35.0 bits (79), Expect = 3.4
 Identities = 28/89 (31%), Positives = 36/89 (40%), Gaps = 1/89 (1%)
 Frame = +2

Query: 165    WAVPGDSSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVAFER 224
              WA PGDS  S  V K   Q+ +   AH L + F  P     ++    +  N A G  F R
Sbjct: 374162 WAFPGDSGTSLMVPKGCGQKQKADLAHLLHKTFPNPQPTAFLSCVRSHIGN*A*GKQFFR 374341

Query: 225    VCIVQRRKIAYLGGKGPFS-YNLQEVRSW 252
               C              PFS   L+ +RSW
Sbjct: 374342 GC--------------PFSPMRLEGIRSW 374386


>gb|JH418183.1| Mustela putorius furo unplaced genomic scaffold scaffold00211, whole
              genome shotgun sequence
          Length = 3280579

 Score = 35.0 bits (79), Expect = 3.4
 Identities = 21/46 (45%), Positives = 28/46 (60%)
 Frame = -3

Query: 6      VDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRSKSTRGEW 51
              V LLI+  I P+   N L L LYDSVI+L  + ++L R +ST   W
Sbjct: 734066 VGLLISPPIHPLSMHNVLILKLYDSVIVL--MIIILQRYRSTSEVW 733935


>gb|JH418114.1| Mustela putorius furo unplaced genomic scaffold scaffold00142, whole
              genome shotgun sequence
          Length = 5569031

 Score = 33.9 bits (76), Expect = 7.6
 Identities = 21/49 (42%), Positives = 28/49 (57%)
 Frame = +3

Query: 171    SSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYG 219
              SS+S  VK  +N     +++H+LL R + P QC V  DR   NA V YG
Sbjct: 511383 SSMSVLVKWPKN-----SSSHRLLNRSA*PLQCSVFLDRTVPNAPVCYG 511514


>gb|JH417993.1| Mustela putorius furo unplaced genomic scaffold scaffold00021, whole genome
                shotgun sequence
          Length = 19809657

 Score = 33.9 bits (76), Expect = 7.6
 Identities = 15/39 (38%), Positives = 24/39 (61%)
 Frame = +3

Query: 4        LSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLS 42
                L +DL+IT+ +L    S CLFL  Y+  +L+K +  + S
Sbjct: 16714428 LVIDLIITISLLIALISLCLFLITYNYTLLIKKIYSIFS 16714544


  Database: /cursos/BI/genomes/project_2013/Mustela_putorius_furo/geno
  me.fa
    Posted date:  Oct 24, 2012  8:21 PM
  Number of letters in database: 2,400,181,698
  Number of sequences in database:  4245
  
Lambda     K      H
   0.321    0.135    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 794,311,819
Number of Sequences: 4245
Number of extensions: 11865367
Number of successful extensions: 51760
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 119
Number of HSP's that attempted gapping in prelim test: 43094
Number of HSP's gapped (non-prelim): 22050
length of query: 264
length of database: 800,060,566
effective HSP length: 128
effective length of query: 136
effective length of database: 799,517,206
effective search space: 108734340016
effective search space used: 108734340016
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 75 (33.5 bits)
