TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|8923460|ref|NP_060316.1| tRNA selenocysteine 1-associated
protein 1 [Homo sapiens]
         (287 letters)

Database: I.multifiliis_strain_G5/genome.fa 
           2017 sequences; 48,799,969 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|340502518|gb|GL984173.1| Ichthyophthirius multifiliis unplace...    47   3e-05
gi|340502067|gb|GL984209.1| Ichthyophthirius multifiliis unplace...    41   0.002
gi|340506643|gb|GL983583.1| Ichthyophthirius multifiliis unplace...    31   1.4  
gi|340500259|gb|GL984386.1| Ichthyophthirius multifiliis unplace...    28   8.8  

>gi|340502518|gb|GL984173.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251212, whole genome shotgun
             sequence
          Length = 78614

 Score = 46.6 bits (109), Expect = 3e-05
 Identities = 28/78 (35%), Positives = 50/78 (64%), Gaps = 1/78 (1%)
 Frame = -1

Query: 98    LFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFVKFTDELEQKRALTE 156
             ++VG+L   +++  L E F   YPS  G K+++D  T +S+G+GFVKF++  E  +A+ E
Sbjct: 30170 VYVGELELGINE**LAEHFRVKYPSVIGSKIIIDP*TKMSRGFGFVKFSNP*EGLKAI*E 29991

Query: 157   CQGAVGLGSKPVRLSVAI 174
               G++  G K +++S A+
Sbjct: 29990 MNGSLFRG-KYIKVSNAV 29940



 Score = 38.5 bits (88), Expect = 0.008
 Identities = 29/123 (23%), Positives = 46/123 (37%), Gaps = 66/123 (53%)
 Frame = -3

Query: 4     SLWMGDLEPYMDENFISRAFATMGET-------------------------------VMS 32
             +LW+GD+EP+M+E ++   F  +G+T                               V+S
Sbjct: 30876 TLWIGDIEPWMNEKYLEDVFNKVGKTLN**YYRFIIYLNYLPILY*KIK*IK*LKGRVVS 30697

Query: 33    VKIIRNRLTGIPA-----------------------------------GYCFVEFADLAT 57
             +K+IRN+  G+P+                                   GYCF+EFA+   
Sbjct: 30696 IKLIRNKDNGLPSGNYIFYFR*IKIYV*IIIFIYIKYIYIYIYIYIYIGYCFIEFANRD* 30517

Query: 58    AEK 60
             AEK
Sbjct: 30516 AEK 30508


>gi|340502067|gb|GL984209.1| Ichthyophthirius multifiliis unplaced genomic
              scaffold scaff_1120509251248, whole genome shotgun sequence
          Length = 207743

 Score = 40.8 bits (94), Expect = 0.002
 Identities = 47/183 (25%), Positives = 86/183 (46%), Gaps = 6/183 (3%)
 Frame = -1

Query: 3      ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAE--- 59
              A++++G ++  + +  +   F+  G  V++V + ++++TG   GY FVEF     A+   
Sbjct: 116615 ATIYVGGID*KVTQEVLWELFS*CG-IVVNVHLPKDKITGEHQGYGFVEFKSEEDADYSI 116439

Query: 60     KCLH--KINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFV 117
              K +H  K+ GKP+         K+N A+  K+       ++F+G+L   + +  L++ F 
Sbjct: 116438 KIMHLVKLYGKPI---------KVNKAS*DKRT*EVGA-NIFIGNLD*SITE**LHDTF- 116292

Query: 118    KVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPK 176
                +      ++V D     SKGY FV + D  E   A          GSK + +  A  K
Sbjct: 116291 S*FGLIISRRIVRDPDNDESKGYAFVSY-DNFEAADAAINTMNG*FFGSKKINV*YAFKK 116115

Query: 177    ASR 179
               S+
Sbjct: 116114 DSK 116106


>gi|340506643|gb|GL983583.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509250402, whole genome shotgun
             sequence
          Length = 74831

 Score = 31.2 bits (69), Expect = 1.4
 Identities = 17/67 (25%), Positives = 29/67 (43%), Gaps = 2/67 (2%)
 Frame = -2

Query: 5     LWMGDLEPYMDENFISRAFATMGETVMS--VKIIRNRLTGIPAGYCFVEFADLATAEKCL 62
             ++MG L  Y+ +  I +   T G+       K        +  GYCF E+ D    ++ +
Sbjct: 34495 IYMGGLPTYLKDPEIKKLCETFGKLKYFNLAK**NENKEWVSKGYCFFEYEDKEVTDRAI 34316

Query: 63    HKINGKP 69
               +NG P
Sbjct: 34315 KALNGLP 34295


>gi|340500259|gb|GL984386.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251425, whole genome shotgun
             sequence
          Length = 90605

 Score = 28.5 bits (62), Expect = 8.8
 Identities = 12/45 (26%), Positives = 22/45 (48%)
 Frame = +2

Query: 30    VMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKINGKPLPGAT 74
             + +  ++++  T    GY F EF D+   + C+  +NG    G T
Sbjct: 73022 IKAFHLVKDN*TNQSKGYAFFEFKDINVTD*CV*SLNGLSYAGRT 73156


  Database: I.multifiliis_strain_G5/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 48,799,969
  Number of sequences in database:  2017
  
Lambda     K      H
   0.317    0.135    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,728,961
Number of Sequences: 2017
Number of extensions: 101906
Number of successful extensions: 127
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 102
Number of HSP's gapped (non-prelim): 27
length of query: 287
length of database: 16,266,656
effective HSP length: 102
effective length of query: 185
effective length of database: 16,060,922
effective search space: 2971270570
effective search space used: 2971270570
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)