TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|224465209|ref|NP_699167.2| L-seryl-tRNA(Sec) kinase [Homo
sapiens]
         (348 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig1754  19362 42502                                                52   6e-06
Contig21074  787 787                                                   33   1.6  

>Contig1754  19362 42502
          Length = 42502

 Score = 51.6 bits (122), Expect = 6e-06
 Identities = 20/56 (35%), Positives = 31/56 (55%)
 Frame = +2

Query: 173   GFCQLFLDCPLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPS 228
             GF Q+ + CP+E C+ RN  R   +P   I  M  + E P P  + WE +++ +PS
Sbjct: 32951 GFAQVHVTCPVEECIARNASRTNPVPESIIRDMAERFEVPAPTSHEWERHTIQVPS 33118


>Contig21074  787 787
          Length = 787

 Score = 33.5 bits (75), Expect = 1.6
 Identities = 19/44 (43%), Positives = 25/44 (56%)
 Frame = +3

Query: 23 LCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVMPDAFLAG 66
          + G P AGKSTF  AL   L QE+   + V+A D   P + L+G
Sbjct: 6  ISGAPGAGKSTFIEALGSYLTQEKHHHVAVLAVD---PSSSLSG 128


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.321    0.134    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 148,080,381
Number of Sequences: 297657
Number of extensions: 2342245
Number of successful extensions: 9849
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 9442
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 9844
length of query: 348
length of database: 106,339,350
effective HSP length: 114
effective length of query: 234
effective length of database: 72,406,452
effective search space: 16943109768
effective search space used: 16943109768
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 68 (30.8 bits)