TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase; Short=PSTK (255 letters) Database: T.congolense/genome.fa 11 sequences; 22,287,946 total letters Searching...........done Score E Sequences producing significant alignments: (bits) Value TcIL3000_10_v2.embl 32 0.24 T.congo.pschr.9 29 2.7 T.congo.pschr2 28 3.5 >TcIL3000_10_v2.embl Length = 3612457 Score = 32.3 bits (72), Expect = 0.24 Identities = 44/183 (24%), Positives = 75/183 (40%), Gaps = 50/183 (27%) Frame = +3 Query: 2 LIILTGLPSVGKSTFSKAFSKKM------------AEKNIDNIIL--------GTDLIRE 41 L++LTGLP GKST ++A ++ A +D+ I+ G+D + + Sbjct: 2047035 LVLLTGLPGAGKSTLARALQRQRSVAGEPSRLSIEAVVELDDFIIAAGAVEDGGSDTLVK 2047214 Query: 42 SF----PVWKESYEE----FIRDSNNYLIKEALEN---KFSVIVDDTNYYNSKRR--DLM 88 S W+E+Y E R+ L + N + VD Y + + L Sbjct: 2047215 STLFTPQRWREAYVESRSAIHRELRRCLTVDEGSNGRLRLVFFVDPLPYRSMRESYWKLC 2047394 Query: 89 N-IAKECD-------------TNYVTIYLKAPLNLLLKRNIERG---QKIPNEVIKNMYE 131 N +++EC + + L P+++ L+RN R Q +P VIK + + Sbjct: 2047395 NEVSRECGELRCVQAPERLNRVLMLEVRLNTPVDVCLRRNEFRSGTPQYVPPYVIKGISD 2047574 Query: 132 KFD 134 FD Sbjct: 2047575 SFD 2047583 >T.congo.pschr.9 Length = 2092392 Score = 28.9 bits (63), Expect = 2.7 Identities = 19/71 (26%), Positives = 36/71 (50%), Gaps = 4/71 (5%) Frame = +1 Query: 3 IILTGLPSVGKSTFSKAFSKKMAEKNIDNIIL-GTDLIRESF---PVWKESYEEFIRDSN 58 ++L+G+P+VGK+T + ++ E + + G D I + VW+ + E F D+ Sbjct: 415090 VLLSGIPAVGKTTMGRELVNELREDGLGWVFFSGADFIATATGKRAVWETTKEVF--DAL 415263 Query: 59 NYLIKEALENK 69 + E LE + Sbjct: 415264 EKRLDELLEQQ 415296 >T.congo.pschr2 Length = 799489 Score = 28.5 bits (62), Expect = 3.5 Identities = 12/19 (63%), Positives = 14/19 (73%) Frame = -3 Query: 5 LTGLPSVGKSTFSKAFSKK 23 + GLP+VGKSTF SKK Sbjct: 586604 IVGLPNVGKSTFFNVLSKK 586548 Database: T.congolense/genome.fa Posted date: Nov 21, 2011 7:47 PM Number of letters in database: 22,287,946 Number of sequences in database: 11 Lambda K H 0.315 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,756,329 Number of Sequences: 11 Number of extensions: 35096 Number of successful extensions: 123 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 107 Number of HSP's gapped (non-prelim): 34 length of query: 255 length of database: 7,429,315 effective HSP length: 95 effective length of query: 160 effective length of database: 7,428,270 effective search space: 1188523200 effective search space used: 1188523200 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 58 (26.9 bits)