TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase; Short=PSTK (255 letters) Database: S.arctica/genome.fa 15,618 sequences; 121,588,341 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value supercont1.4674 of Sphaeroforma arctica JP610 32 2.0 supercont1.553 of Sphaeroforma arctica JP610 29 9.9 >supercont1.4674 of Sphaeroforma arctica JP610 Length = 2338 Score = 31.6 bits (70), Expect = 2.0 Identities = 11/46 (23%), Positives = 25/46 (54%) Frame = +2 Query: 72 VIVDDTNYYNSKRRDLMNIAKECDTNYVTIYLKAPLNLLLKRNIER 117 +++DD YY S R+++ +A YV +++ + ++R+ R Sbjct: 1220 ILIDDNFYYRSMRKEIYQLADIMSIGYVQVWIVCDTEVAVRRDARR 1357 Score = 30.4 bits (67), Expect = 4.5 Identities = 14/35 (40%), Positives = 22/35 (62%), Gaps = 1/35 (2%) Frame = +1 Query: 120 KIPNEVIKNMYEKFDTPGTK-YAWDLPDITVDTTE 153 ++ EVI+ M +FDTP +AW+ I +DTT+ Sbjct: 2071 RVGAEVIQRMSARFDTPDPHGFAWEKHTICIDTTD 2175 >supercont1.553 of Sphaeroforma arctica JP610 Length = 60770 Score = 29.3 bits (64), Expect = 9.9 Identities = 15/43 (34%), Positives = 23/43 (53%) Frame = +2 Query: 61 LIKEALENKFSVIVDDTNYYNSKRRDLMNIAKECDTNYVTIYL 103 ++K L VI D NY SK +++I CD Y+T+Y+ Sbjct: 42956 IVKHGLHVSHEVIQDIFNYSESKA--IISITIFCDNVYITVYM 43078 Database: S.arctica/genome.fa Posted date: Nov 21, 2011 7:47 PM Number of letters in database: 121,588,341 Number of sequences in database: 15,618 Lambda K H 0.315 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 22,004,199 Number of Sequences: 15618 Number of extensions: 231023 Number of successful extensions: 690 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 482 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 174 Number of HSP's gapped (non-prelim): 544 length of query: 255 length of database: 40,529,447 effective HSP length: 107 effective length of query: 148 effective length of database: 38,858,321 effective search space: 5751031508 effective search space used: 5751031508 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 64 (29.3 bits)