TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName:
Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec)
kinase; Short=PSTK
         (255 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig29865  1749 1749                                                 37   0.067
Contig405  28860 28940                                                 37   0.067
Contig288798  722 722                                                  31   6.3  

>Contig29865  1749 1749
          Length = 1749

 Score = 37.4 bits (85), Expect = 0.067
 Identities = 28/98 (28%), Positives = 48/98 (48%), Gaps = 7/98 (7%)
 Frame = +2

Query: 3    IILTGLPSVGKSTFSKAFSKKMAE----KNIDNIILGTDLIRESFPVWKESYEEFIRDSN 58
            I++ G P  GK++ +    KK+ +    + I   +LG D  +E    W E +E  I +  
Sbjct: 1349 ILVCGPPFSGKTSLAYLLQKKLRQNTSIRTIKFSMLGMDTTKE----WTEMWESKILEKW 1516

Query: 59   NYLIKEALENKFSVIVDD---TNYYNSKRRDLMNIAKE 93
            +Y I+++ E    +I+D+   T Y  +K   L N  KE
Sbjct: 1517 SYTIEKSKEESTYIIIDEGQMTYYDINKYAALWNFIKE 1630


>Contig405  28860 28940
          Length = 28940

 Score = 37.4 bits (85), Expect = 0.067
 Identities = 28/98 (28%), Positives = 48/98 (48%), Gaps = 7/98 (7%)
 Frame = +2

Query: 3     IILTGLPSVGKSTFSKAFSKKMAE----KNIDNIILGTDLIRESFPVWKESYEEFIRDSN 58
             I++ G P  GK++ +    KK+ +    + I   +LG D  +E    W E +E  I +  
Sbjct: 22667 ILVCGPPFSGKTSLAYLLQKKLRQNTSIRTIKFSMLGMDTTKE----WTEMWESKILEKW 22834

Query: 59    NYLIKEALENKFSVIVDD---TNYYNSKRRDLMNIAKE 93
             +Y I+++ E    +I+D+   T Y  +K   L N  KE
Sbjct: 22835 SYTIEKSKEESTYIIIDEGQMTYYDINKYAALWNFIKE 22948


>Contig288798  722 722
          Length = 722

 Score = 30.8 bits (68), Expect = 6.3
 Identities = 14/59 (23%), Positives = 30/59 (50%)
 Frame = +1

Query: 50  YEEFIRDSNNYLIKEALENKFSVIVDDTNYYNSKRRDLMNIAKECDTNYVTIYLKAPLN 108
           ++ F+   NNY +K  ++NK   ++  ++ Y   ++D+  + K     + TI    P+N
Sbjct: 535 HQFFLLHRNNYNMKIKIDNKAIQVLKSSDGYRKWKQDVNMLVKNFTQTFGTISFSLPMN 711


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.315    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,022,385
Number of Sequences: 297657
Number of extensions: 639395
Number of successful extensions: 3331
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 3254
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3325
length of query: 255
length of database: 106,339,350
effective HSP length: 111
effective length of query: 144
effective length of database: 73,299,423
effective search space: 10555116912
effective search space used: 10555116912
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 66 (30.0 bits)