TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase; Short=PSTK (255 letters) Database: L.tarentolae/genome.fa 7267 sequences; 31,598,840 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Lt_contig3390 | | 1 to 2944 30 1.6 Lt_contig5262 | | 1 to 11693 29 3.5 Lt_contig4824 | | 1 to 14951 29 3.5 Lt_contig1459 | | 1 to 10944 28 7.8 >Lt_contig3390 | | 1 to 2944 Length = 2944 Score = 30.0 bits (66), Expect = 1.6 Identities = 12/26 (46%), Positives = 19/26 (73%) Frame = -2 Query: 220 EIRKSFLKDCKKIESEKLDFEKIEKD 245 E+R S LKDCK+ ++EK EK +++ Sbjct: 2172 ELRSSHLKDCKRAKAEKEKLEKAKEE 2095 >Lt_contig5262 | | 1 to 11693 Length = 11693 Score = 28.9 bits (63), Expect = 3.5 Identities = 12/24 (50%), Positives = 17/24 (70%) Frame = -3 Query: 2 LIILTGLPSVGKSTFSKAFSKKMA 25 +++L GLP GKST S A S+ +A Sbjct: 10038 VVVLCGLPGAGKSTLSVALSRLLA 9967 >Lt_contig4824 | | 1 to 14951 Length = 14951 Score = 28.9 bits (63), Expect = 3.5 Identities = 12/24 (50%), Positives = 17/24 (70%) Frame = -3 Query: 2 LIILTGLPSVGKSTFSKAFSKKMA 25 +++L GLP GKST S A S+ +A Sbjct: 13296 VVVLCGLPGAGKSTLSVALSRLLA 13225 >Lt_contig1459 | | 1 to 10944 Length = 10944 Score = 27.7 bits (60), Expect = 7.8 Identities = 11/19 (57%), Positives = 14/19 (73%) Frame = +3 Query: 5 LTGLPSVGKSTFSKAFSKK 23 + GLP++GKSTF SKK Sbjct: 8253 IVGLPNIGKSTFFNILSKK 8309 Database: L.tarentolae/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 31,598,840 Number of sequences in database: 7267 Lambda K H 0.315 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,453,441 Number of Sequences: 7267 Number of extensions: 34109 Number of successful extensions: 153 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of query: 255 length of database: 10,532,946 effective HSP length: 97 effective length of query: 158 effective length of database: 9,828,047 effective search space: 1552831426 effective search space used: 1552831426 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 59 (27.3 bits)