TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName:
Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec)
kinase; Short=PSTK
         (255 letters)

Database: I.multifiliis_strain_G5/genome.fa 
           2017 sequences; 48,799,969 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|340505368|gb|GL983824.1| Ichthyophthirius multifiliis unplace...    33   0.30 
gi|340507142|gb|GL983505.1| Ichthyophthirius multifiliis unplace...    31   1.1  
gi|340502067|gb|GL984209.1| Ichthyophthirius multifiliis unplace...    30   2.5  
gi|340503839|gb|GL984007.1| Ichthyophthirius multifiliis unplace...    28   7.3  
gi|340502239|gb|GL984200.1| Ichthyophthirius multifiliis unplace...    28   9.5  

>gi|340505368|gb|GL983824.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509250860, whole genome shotgun
             sequence
          Length = 116945

 Score = 33.1 bits (74), Expect = 0.30
 Identities = 30/81 (37%), Positives = 39/81 (48%), Gaps = 2/81 (2%)
 Frame = +1

Query: 17    SKAFSKKMAEKNIDNIILGTDLIRESFPVWKESYEEFIRDSNNYLIKEA--LENKFSVIV 74
             +K   KKM      N+ILG  L + SF + KE Y  FI+   N L  E+  L+ K SV  
Sbjct: 26170 NKIIEKKMGTTLSHNMILGD*LEQTSFNIHKECY--FIKKIENNLFGESQLLQQKESV-- 26337

Query: 75    DDTNYYNSKRRDLMNIAKECD 95
               TN Y   +  LM+   E D
Sbjct: 26338 --TNPYYILQSRLMSTKSEYD 26394


>gi|340507142|gb|GL983505.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509250274, whole genome shotgun
             sequence
          Length = 78887

 Score = 31.2 bits (69), Expect = 1.1
 Identities = 15/32 (46%), Positives = 21/32 (65%)
 Frame = +1

Query: 78    NYYNSKRRDLMNIAKECDTNYVTIYLKAPLNL 109
             N+Y SKRRD+ NI K  +T+   I+ +  LNL
Sbjct: 74386 NFYLSKRRDVENICKWMETSLSDIFKEKNLNL 74481


>gi|340502067|gb|GL984209.1| Ichthyophthirius multifiliis unplaced genomic
              scaffold scaff_1120509251248, whole genome shotgun sequence
          Length = 207743

 Score = 30.0 bits (66), Expect = 2.5
 Identities = 24/86 (27%), Positives = 42/86 (48%), Gaps = 8/86 (9%)
 Frame = -1

Query: 2      LIILTGLPSVGKSTFSKAFSKKMAEKNIDNIILGTDLIR--ESFPVWKESYEEFIRDSN- 58
              +++  G+P +GKS F +   +   +K +   I+ +D IR  E     K ++EE    +  
Sbjct: 178958 VLLPLGIPGMGKSIFFRKIKETWTKKGV*VEIISSDSIRE*EMKKEKKGNFEEKFERTGK 178779

Query: 59     --NYLIKEALENKF---SVIVDDTNY 79
                NY+ K+ +  K     VI+ D NY
Sbjct: 178778 RANYIFKKEV*EKLKENKVILLDKNY 178701


>gi|340503839|gb|GL984007.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251045, whole genome shotgun
             sequence
          Length = 67139

 Score = 28.5 bits (62), Expect = 7.3
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = +1

Query: 52    EFIRDSNNYLIKEALENKFSVIVDD 76
             + I ++NNY+    + NKFS+I+DD
Sbjct: 51613 KIINNNNNYINHININNKFSIILDD 51687


>gi|340502239|gb|GL984200.1| Ichthyophthirius multifiliis unplaced
            genomic scaffold scaff_1120509251239, whole genome
            shotgun sequence
          Length = 40045

 Score = 28.1 bits (61), Expect = 9.5
 Identities = 11/21 (52%), Positives = 16/21 (76%)
 Frame = +2

Query: 235  EKLDFEKIEKDFLDYLNKNLK 255
            +K   +K+  DFL+Y+NKNLK
Sbjct: 7976 KKFPKKKVNLDFLNYINKNLK 8038


  Database: I.multifiliis_strain_G5/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 48,799,969
  Number of sequences in database:  2017
  
Lambda     K      H
   0.315    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,549,673
Number of Sequences: 2017
Number of extensions: 251800
Number of successful extensions: 753
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 43
Number of HSP's that attempted gapping in prelim test: 573
Number of HSP's gapped (non-prelim): 280
length of query: 255
length of database: 16,266,656
effective HSP length: 101
effective length of query: 154
effective length of database: 16,062,939
effective search space: 2473692606
effective search space used: 2473692606
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 61 (28.1 bits)