TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase; Short=PSTK (255 letters) Database: C.fasciculata/genome.fa 1089 sequences; 37,048,325 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value Cf_Contig631 | | 1 to 5605 28 5.6 Cf_Contig765 | | 1 to 162644 28 9.6 Cf_Contig153 | | 1 to 16578 28 9.6 >Cf_Contig631 | | 1 to 5605 Length = 5605 Score = 28.5 bits (62), Expect = 5.6 Identities = 12/19 (63%), Positives = 14/19 (73%) Frame = +1 Query: 5 LTGLPSVGKSTFSKAFSKK 23 + GLP+VGKSTF SKK Sbjct: 2608 IVGLPNVGKSTFFNVLSKK 2664 >Cf_Contig765 | | 1 to 162644 Length = 162644 Score = 27.7 bits (60), Expect = 9.6 Identities = 11/16 (68%), Positives = 14/16 (87%) Frame = +3 Query: 1 MLIILTGLPSVGKSTF 16 +LI+LTGLP GKS+F Sbjct: 55728 LLILLTGLPGAGKSSF 55775 >Cf_Contig153 | | 1 to 16578 Length = 16578 Score = 27.7 bits (60), Expect = 9.6 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = -2 Query: 43 FPVWKESYEEFIRDSNNYLIKEALENKFS 71 FPVWK EE ++ N ++ EA++ + + Sbjct: 7547 FPVWKHPSEERLQAHNKKILSEAMKRRMA 7461 Database: C.fasciculata/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 37,048,325 Number of sequences in database: 1089 Lambda K H 0.315 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,237,595 Number of Sequences: 1089 Number of extensions: 43698 Number of successful extensions: 146 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 122 Number of HSP's gapped (non-prelim): 44 length of query: 255 length of database: 12,349,441 effective HSP length: 99 effective length of query: 156 effective length of database: 12,241,630 effective search space: 1909694280 effective search space used: 1909694280 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 60 (27.7 bits)