TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000085_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Caenorhabditis elegans # Complete
         (500 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig14005  2832 2842                                                 55   6e-07
Contig19809  1437 2007                                                 54   1e-06
Contig12363  2234 2234                                                 42   1e-05
Contig13961  1573 1573                                                 43   0.003
Contig4246  8897 9149                                                  43   0.003
Contig6188  7064 14978                                                 42   0.007
Contig604  29324 51689                                                 35   0.86 
Contig3376  11693 11773                                                34   1.9  

>Contig14005  2832 2842
          Length = 2842

 Score = 55.5 bits (132), Expect = 6e-07
 Identities = 29/77 (37%), Positives = 45/77 (58%), Gaps = 1/77 (1%)
 Frame = -2

Query: 62   GFSTMTSLSGRRLALIDCPGHSGLIRAVLAASTVFDMAIVIIDVVAGI-QPQTAEHLLLA 120
            G     +L  R ++ +DCPGH  L+R +L  ++V D AI+++     + QPQTAEHL++A
Sbjct: 2838 GAGVELTLRYRHMSFVDCPGHEMLMRTMLNGASVMDTAILVVAADQKVPQPQTAEHLVVA 2659

Query: 121  SKFCPNRVIIVLNKCDL 137
                   ++ V NK DL
Sbjct: 2658 EIMGLPNILAVQNKVDL 2608


>Contig19809  1437 2007
          Length = 2007

 Score = 54.3 bits (129), Expect = 1e-06
 Identities = 26/58 (44%), Positives = 37/58 (63%)
 Frame = -1

Query: 174  YFKEEMLQNLKLAIESRIFEPKRDEEGEFLIAIDHCFAIKGQGTVLTGTVIRGVLRLN 231
            YF +E+   +   +  ++ +P+R  EG  L   DHCF IKGQGTVLTGTV++G +  N
Sbjct: 1938 YFFDEIYVAI---LAQKVAQPQRSGEGPLLFEFDHCFPIKGQGTVLTGTVLKGSISGN 1774


>Contig12363  2234 2234
          Length = 2234

 Score = 41.6 bits (96), Expect(2) = 1e-05
 Identities = 45/172 (26%), Positives = 69/172 (40%), Gaps = 19/172 (11%)
 Frame = -1

Query: 97   DMAIVII-----DVVAGIQP--QTAEHLLLASKFCPNRVIIVLNKCDLXXXXXXXXXXXX 149
            D A+++I     +  AGI    QT EH LLA      ++I+ +NK D             
Sbjct: 1040 DAAVLVIASPTGEFEAGIAKTGQTREHALLAYTLGVKQMIVAINKMDEKSVNWSQARYDE 861

Query: 150  VRKGLKSM--GVDENSPIVEMSLADGYFKEEMLQ---NLK-------LAIESRIFEPKRD 197
            + K   S    +  N   +      G+  + ML+   NL        L    +I EPKR 
Sbjct: 860  IVKETSSFVKKIGYNPEKIAFVPISGWNGDNMLEKSANLPWYKGPTLLEALDQITEPKRP 681

Query: 198  EEGEFLIAIDHCFAIKGQGTVLTGTVIRGVLRLNTEIEFPALNEKRRVKTLE 249
             +    + +   + I G GTV  G V  G+L+    + F   N    VK++E
Sbjct: 680  TDKPLRVPLQDVYKIGGIGTVPVGRVETGILKPGMIVTFAPANLSTEVKSVE 525



 Score = 29.3 bits (64), Expect(2) = 1e-05
 Identities = 24/108 (22%), Positives = 44/108 (40%), Gaps = 20/108 (18%)
 Frame = -2

Query: 14   LNLGILGHVDSGKTTLT-------------------RRIAELGSTSAFDAHATSTTTDGI 54
            +N+ ++GHVD+GK+T T                   +  AE+G  S   A          
Sbjct: 1450 INIVVIGHVDAGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKSER 1271

Query: 55   RRN-TLDLGFSTMTSLSGRRLALIDCPGHSGLIRAVLAASTVFDMAIV 101
             R  T+D+      + +   + +ID PGH   I+ ++  ++    + V
Sbjct: 1270 ERGITIDIALWKFET-AKYYITIIDAPGHRDFIKNMITGTSQVSFSFV 1130


>Contig13961  1573 1573
          Length = 1573

 Score = 43.1 bits (100), Expect = 0.003
 Identities = 21/35 (60%), Positives = 26/35 (74%)
 Frame = -2

Query: 14  LNLGILGHVDSGKTTLTRRIAELGSTSAFDAHATS 48
           +N+GILGHVDSGKT+L R I+ L ST+A D    S
Sbjct: 141 VNVGILGHVDSGKTSLARAISTLLSTAALDKSPAS 37


>Contig4246  8897 9149
          Length = 9149

 Score = 43.1 bits (100), Expect = 0.003
 Identities = 19/49 (38%), Positives = 35/49 (71%)
 Frame = +1

Query: 187  IESRIFEPKRDEEGEFLIAIDHCFAIKGQGTVLTGTVIRGVLRLNTEIE 235
            ++++I +PKR  +  FL+ I+  FAI G+GTV+TG + +G L++  +I+
Sbjct: 5431 LDNKIPQPKRPIDKPFLMPIEDIFAITGRGTVVTGRLEQGTLKVGEQIQ 5577



 Score = 40.8 bits (94), Expect = 0.016
 Identities = 28/92 (30%), Positives = 41/92 (44%), Gaps = 22/92 (23%)
 Frame = +3

Query: 68   SLSGRRLALIDCPGHSGLIRAVLAA-----STVFDM-----------------AIVIIDV 105
            S   R  A IDCPGH   I+ ++       S +F +                 AI+++  
Sbjct: 4686 STENRHYAHIDCPGHQHYIKNMITGAAQV*SCLFSILLSLAHPLFFFVLQMEGAILVVSA 4865

Query: 106  VAGIQPQTAEHLLLASKFCPNRVIIVLNKCDL 137
              G Q QT EH++L+ +     V+I LNK DL
Sbjct: 4866 PDGPQEQTREHIILSREVGVPHVVIFLNKMDL 4961


>Contig6188  7064 14978
          Length = 14978

 Score = 42.0 bits (97), Expect = 0.007
 Identities = 41/168 (24%), Positives = 65/168 (38%), Gaps = 26/168 (15%)
 Frame = -3

Query: 72   RRLALIDCPGHSGLIRAVLAASTVFDMAIVIIDVVAGI---------------------- 109
            R ++ +DCPGH  L+  +L  + V D A+++I    G+                      
Sbjct: 4992 RHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGELGVRRDIRTRKEKLHLTQY*YYAGN 4813

Query: 110  ----QPQTAEHLLLASKFCPNRVIIVLNKCDLXXXXXXXXXXXXVRKGLKSMGVDENSPI 165
                QPQT+EHL          +II+ NK DL            + K +K +    +SP 
Sbjct: 4812 ESCPQPQTSEHLAAIEIMKLKHIIILQNKIDLIKDTAASEQYQQILKFIKGL----SSPC 4645

Query: 166  VEMSLADGYFKEEMLQNLKLAIESRIFEPKRDEEGEFLIAIDHCFAIK 213
              +    GY K   L   K  I   +    RD +   ++ +  CF +K
Sbjct: 4644 YLIGYL-GYSKSITLSVRKFCIIRTL--DHRDSQPLIVLTV-FCFLLK 4513


>Contig604  29324 51689
          Length = 51689

 Score = 35.0 bits (79), Expect = 0.86
 Identities = 24/89 (26%), Positives = 41/89 (46%), Gaps = 10/89 (11%)
 Frame = -1

Query: 58   TLDLG---FSTMTSLSGRRLALIDCPGHSGLIRAVLAASTVFDMAIVIIDVVAG------ 108
            T+++G   F T T    +R  ++D PGH   +  ++  +   D+AI++I    G      
Sbjct: 1112 TVEVGRAHFETKT----KRYTILDAPGHKNYVPNMIGGAAQADVAILVISARKGEFETGF 945

Query: 109  -IQPQTAEHLLLASKFCPNRVIIVLNKCD 136
                QT EH  L        +++V+NK D
Sbjct: 944  EKGGQTREHARLVKTIGVKYLLVVINKMD 858


>Contig3376  11693 11773
          Length = 11773

 Score = 33.9 bits (76), Expect = 1.9
 Identities = 14/19 (73%), Positives = 16/19 (84%)
 Frame = +2

Query: 15   NLGILGHVDSGKTTLTRRI 33
            N+GI  H+DSGKTTLT RI
Sbjct: 9638 NIGISAHIDSGKTTLTERI 9694


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.322    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 161,261,317
Number of Sequences: 297657
Number of extensions: 2169426
Number of successful extensions: 10616
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 9474
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10342
length of query: 500
length of database: 106,339,350
effective HSP length: 118
effective length of query: 382
effective length of database: 71,215,824
effective search space: 27204444768
effective search space used: 27204444768
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 70 (31.6 bits)