TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000108_1.0 # Protein # Selenophosphate synthetase 2 (SPS2) # Mus musculus # Complete (452 letters) Database: S.arctica/genome.fa 15,618 sequences; 121,588,341 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value supercont1.927 of Sphaeroforma arctica JP610 87 7e-17 >supercont1.927 of Sphaeroforma arctica JP610 Length = 30644 Score = 87.4 bits (215), Expect = 7e-17 Identities = 41/78 (52%), Positives = 53/78 (67%) Frame = +2 Query: 221 NPWIIIGGVATVVCQQNEFIMPDSAVVGDVLVLTKPLGTQVAANAHQWLDNPEKWNKIKM 280 NPW I+GGVA V + I P++A GDV+VLTKPLGTQ+A N HQWLD P+ W+++K Sbjct: 3908 NPWPILGGVAQSVMLATDVITPENARAGDVMVLTKPLGTQLAVNLHQWLDQPKWWDQVKH 4087 Query: 281 VVSREEVELAYQEAMFNM 298 V+ E V AY A +M Sbjct: 4088 VIDAETVIRAYNVASASM 4141 Score = 67.8 bits (164), Expect = 6e-11 Identities = 29/43 (67%), Positives = 36/43 (83%) Frame = +3 Query: 133 GLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDN 175 G+ +V TTDFFYPLV+DPY G+IACANVLSD+YA+G + DN Sbjct: 2586 GVVMVSTTDFFYPLVDDPYTQGQIACANVLSDMYAVGARKVDN 2714 Score = 58.9 bits (141), Expect = 3e-08 Identities = 28/48 (58%), Positives = 35/48 (72%) Frame = +1 Query: 301 LNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQKNEVSFVIHNLP 348 LN A LMH NA AATD+TGFGILGH+ NLA+ QK +++ I +LP Sbjct: 4594 LNMVGAQLMHKHNARAATDVTGFGILGHANNLAQAQKAKLTLRIDSLP 4737 Score = 41.2 bits (95), Expect = 0.006 Identities = 22/47 (46%), Positives = 29/47 (61%) Frame = +3 Query: 363 FGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGI 409 F L G SAETSGGLL+C+ A FC I + EG +A++VG+ Sbjct: 6126 FNLATGYSAETSGGLLMCMAPADADAFCRAIHAE---EGWKAFVVGM 6257 Database: S.arctica/genome.fa Posted date: Nov 21, 2011 7:47 PM Number of letters in database: 121,588,341 Number of sequences in database: 15,618 Lambda K H 0.319 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 49,771,989 Number of Sequences: 15618 Number of extensions: 640966 Number of successful extensions: 2076 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 538 Number of HSP's successfully gapped in prelim test: 62 Number of HSP's that attempted gapping in prelim test: 1425 Number of HSP's gapped (non-prelim): 890 length of query: 452 length of database: 40,529,447 effective HSP length: 113 effective length of query: 339 effective length of database: 38,764,613 effective search space: 13141203807 effective search space used: 13141203807 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 67 (30.4 bits)