TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000108_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Mus musculus # Complete
         (452 letters)

Database: I.multifiliis_strain_G5/genome.fa 
           2017 sequences; 48,799,969 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplace...    81   1e-47

>gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251335, whole genome shotgun
             sequence
          Length = 70427

 Score = 80.9 bits (198), Expect(3) = 1e-47
 Identities = 56/148 (37%), Positives = 71/148 (47%), Gaps = 6/148 (4%)
 Frame = -3

Query: 33    RSFSNYRP-----FEPQTLGFSPSWRLTSFSGMKGUGCKVPQETLLKLLEGLTRPALQPP 87
             + + N RP     + P       ++ LT +S +KG GCKVPQ  L K LE L        
Sbjct: 41880 KPYINGRP*KRPLWNPTDHNLEKTFVLTDYSDLKG*GCKVPQADLKKYLEKL-------- 41725

Query: 88    LTSXXXXXXXXXXXXXXXSTRPGPGSAFPSLSIGMDSCVIPLRHGGLS-LVQTTDFFYPL 146
                                     G+A    S   D  V P+R+     L+ T DFFYPL
Sbjct: 41724 ------------------------GNAK*ITSEAPDVSVRPVRNTKTKKLISTIDFFYPL 41617

Query: 147   VEDPYMMGRIACANVLSDLYAMGITECD 174
             VEDPYM GRIAC NVLSDL++MG+T+ D
Sbjct: 41616 VEDPYM*GRIACCNVLSDLFSMGVTDID 41533



 Score = 75.5 bits (184), Expect(3) = 1e-47
 Identities = 47/122 (38%), Positives = 65/122 (53%)
 Frame = -1

Query: 308   LMHTFNAHAATDITGFGILGHSQNLAKQQKNEVSFVIHNLPXXXXXXXXXXXXGRFGLLQ 367
             LM  + A+A TD+TGFGI GHS  LA+  K  V  +I+NLP              F    
Sbjct: 41075 LMKKYKANACTDVTGFGIKGHS*FLAEV*KE*VDLIINNLPTIKYVASIDGKARDFKFK* 40896

Query: 368   GTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIE 427
             G S ETSGGLLICL + +A  F SE+K +    G +  +VG V KG   + + +   + E
Sbjct: 40895 GLSPETSGGLLICLEKNKAKEFISEMKDN----GLECNLVGEVVKGKNNSIVREDASIEE 40728

Query: 428   VL 429
             ++
Sbjct: 40727 II 40722



 Score = 73.9 bits (180), Expect(3) = 1e-47
 Identities = 46/111 (41%), Positives = 63/111 (56%), Gaps = 2/111 (1%)
 Frame = -2

Query: 194   VTPLMIKGFRDXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQQNEFIMPDSAVVGDVLVL 253
             VT LMI GF +                NPW IIGGV   V ++ EF+MP++A VGD+++L
Sbjct: 41422 VTSLMI*GFDECA**VGAYVLGG*TIINPWPIIGGVGISVVEREEFLMPNNAKVGDMIIL 41243

Query: 254   TKPLGTQVAANAHQWL--DNPEKWNKIKMVVSREEVELAYQEAMFNMATLN 302
             TKPLGT +A N   W   D  +K  KI   +S+E V+ A+ +A   M TL+
Sbjct: 41242 TKPLGT*LAVNLK*WYTKDIKDKLEKI*GFISKE*VD*AFLKAEMEMGTLS 41090


  Database: I.multifiliis_strain_G5/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 48,799,969
  Number of sequences in database:  2017
  
Lambda     K      H
   0.319    0.136    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,338,156
Number of Sequences: 2017
Number of extensions: 100967
Number of successful extensions: 97
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 90
Number of HSP's gapped (non-prelim): 9
length of query: 452
length of database: 16,266,656
effective HSP length: 106
effective length of query: 346
effective length of database: 16,052,854
effective search space: 5554287484
effective search space used: 5554287484
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)