TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000108_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Mus musculus # Complete
         (452 letters)

Database: F.cylindrus/genome.fa 
           271 sequences; 80,540,407 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_21                                                           218   2e-56

>scaffold_21
          Length = 951085

 Score =  218 bits (554), Expect = 2e-56
 Identities = 129/335 (38%), Positives = 170/335 (50%), Gaps = 31/335 (9%)
 Frame = -2

Query: 125    CVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNXXXXXXXXX 184
              C I     G  ++ TTDFF+PLV+ PY+ GRI  ANVLSDLYA G+  CD          
Sbjct: 153033 CSIRETRQGHYVISTTDFFFPLVDSPYLQGRIGAANVLSDLYAEGVQHCDFVLMLLAASL 152854

Query: 185    XXXEKEREKVTPLMIKGFRDXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQQNEFIMPDS 244
                  ++R   T LM++GFRD                NPW IIGGVAT V  ++E++  D 
Sbjct: 152853 DMTPQDRTICTNLMVRGFRDACTEAETTITGGQTVLNPWAIIGGVATSVVTRDEYVSSDG 152674

Query: 245    AVVGDVLVLTKPLGTQVAANAHQWLDNPEK------------WNKI-------KMVVSRE 285
              A VGDV+VLTKP+GTQ+A N H+W    EK            WNKI          ++  
Sbjct: 152673 ACVGDVIVLTKPIGTQIAVNVHEWRKRHEKKAKNGDTTGDKNWNKILEQNKNGDEFLTVS 152494

Query: 286    EVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQKNEVSFVIH 345
              E E+    A+ +M+ LNR  + LM   NAHA TD+TGFG+LGH+QNL   Q  +V   +H
Sbjct: 152493 ESEIMMHAAVRSMSKLNRFGSKLMTKHNAHAGTDVTGFGLLGHAQNLMDNQIAKVGIELH 152314

Query: 346    NLPXXXXXXXXXXXXGRFGLLQGTSAETSGGLLICLPREQAARFCSEIK----------- 394
                P              F L  G SAETSGGL+I +    A  +C+E++           
Sbjct: 152313 TFPCIANTLKINDNIMNFRLRDGYSAETSGGLMISMTEADAIAYCAELREVETVAYNYNT 152134

Query: 395    SSKYGEGHQAWIVG-IVEKGNRTARIIDKPRVIEV 428
              SS  G     WI+G +VE  +R A I +    +EV
Sbjct: 152133 SSDAGTDFGPWIIGRVVESADRKATITEDAVYLEV 152029



 Score = 41.6 bits (96), Expect = 0.003
 Identities = 17/39 (43%), Positives = 23/39 (58%)
 Frame = -1

Query: 41     FEPQTLGFSPSWRLTSFSGMKGUGCKVPQETLLKLLEGL 79
              F+P ++G    W LT FS +KG  CK+PQ  L  +L  L
Sbjct: 153418 FDPTSVGLPEGWSLTDFSDLKG*ACKIPQNELQDMLSSL 153302


  Database: F.cylindrus/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 80,540,407
  Number of sequences in database:  271
  
Lambda     K      H
   0.319    0.136    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,211,430
Number of Sequences: 271
Number of extensions: 364766
Number of successful extensions: 1054
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1036
Number of HSP's gapped (non-prelim): 56
length of query: 452
length of database: 26,846,802
effective HSP length: 110
effective length of query: 342
effective length of database: 26,816,992
effective search space: 9171411264
effective search space used: 9171411264
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 66 (30.0 bits)