TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000108_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Mus musculus # Complete
         (452 letters)

Database: D.fasciculatum/genome.fa 
           25 sequences; 31,019,208 total letters

Searching.........................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|328864870|gb|GL883029.1| Dictyostelium fasciculatum unplaced ...   231   6e-66
gi|328869808|gb|GL883018.1| Dictyostelium fasciculatum unplaced ...    30   4.8  

>gi|328864870|gb|GL883029.1| Dictyostelium fasciculatum unplaced genomic
              scaffold Scf_EUAJFUG02HR96I-5, whole genome shotgun
              sequence
          Length = 3408800

 Score =  231 bits (588), Expect(2) = 6e-66
 Identities = 137/320 (42%), Positives = 175/320 (54%), Gaps = 31/320 (9%)
 Frame = -2

Query: 142    FFYPLVEDPYMMGRIACANVLSDLYAMGITECDNXXXXXXXXXXXXEKEREKVTPLMIKG 201
              FF+P  +DPY+MG+IACANVLSDLY+ GI +CDN             ++R   T  MI G
Sbjct: 338566 FFFPNCDDPYLMGKIACANVLSDLYSFGIADCDNMLMILAGSTDMTPEQRTWSTRGMING 338387

Query: 202    FR------------------------DXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQQN 237
              F                         D                NPW IIGGVAT V +++
Sbjct: 338386 FNGIYLHQR**FFLLFINLFYFFYSLDNAKIAGTNITGGQTVKNPWPIIGGVATSVVKKD 338207

Query: 238    EFIMPDSAVVGDVLVLTKPLGTQVAANAHQWLDN-PEKWNKIKMVVSREEVELAYQEAMF 296
              EFIMP  AV GDVLVLTKPLGTQV ANAHQWL   PEKW +I  VV+RE++   +  A+ 
Sbjct: 338206 EFIMPVGAVAGDVLVLTKPLGTQVCANAHQWLQGFPEKWERITGVVTREQIIECWAYAVN 338027

Query: 297    NMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQKNEVSFVIHNLPXXXXXXXX 356
              +MA LNRT A +M    AHA TD+TGFG+LGHS NLAK Q   V F I  LP        
Sbjct: 338026 SMARLNRTGARMMMKHGAHACTDVTGFGLLGHSMNLAKNQNAAVYFEIDTLPIMQGMVEI 337847

Query: 357    XXXXG-RFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNR 415
                     + LL+G SAETSGGLL+ LP ++A  F  +++     +   AWI+G V+  ++
Sbjct: 337846 DATLNYNYNLLKGMSAETSGGLLVALPADKAQAFIDDMQEI---DKQPAWIIGKVKASDK 337676

Query: 416    T-----ARIIDKPRVIEVLP 430
                    + ++D   +I VLP
Sbjct: 337675 PQSENYSYLVDNLNIINVLP 337616



 Score = 39.3 bits (90), Expect(2) = 6e-66
 Identities = 27/89 (30%), Positives = 37/89 (41%)
 Frame = -1

Query: 53     RLTSFSGMKGUGCKVPQETLLKLLEGLTRPALQPPLTSXXXXXXXXXXXXXXXSTRPGPG 112
              +LT F+ +KG GCK+PQ+ LL LLEG+                              G G
Sbjct: 338813 KLTDFTKLKG*GCKIPQKELLSLLEGI------------------------------GSG 338724

Query: 113    SAFPSLSIGMDSCVIPLRHGGLSLVQTTD 141
                     IG D  V+P +   + +V TTD
Sbjct: 338723 -------IGFDCSVVPTKFNDIVMVSTTD 338658


>gi|328869808|gb|GL883018.1| Dictyostelium fasciculatum unplaced genomic
              scaffold Scf_Dfasci-G-b-285a01.f1-5, whole genome shotgun
              sequence
          Length = 1327167

 Score = 29.6 bits (65), Expect = 4.8
 Identities = 16/64 (25%), Positives = 33/64 (51%)
 Frame = -1

Query: 276    NKIKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQ 335
              N    ++ + E ++++Q+   N +TLN +   L    N  + ++     IL +S +  +Q
Sbjct: 550287 NDQTQLIQKLEEDISHQDHPSNNSTLNNSNNNLSSLINESSGSNQNNSPILSNSTDQQQQ 550108

Query: 336    QKNE 339
              QK+E
Sbjct: 550107 QKSE 550096


  Database: D.fasciculatum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 31,019,208
  Number of sequences in database:  25
  
Lambda     K      H
   0.319    0.136    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,758,960
Number of Sequences: 25
Number of extensions: 141008
Number of successful extensions: 421
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 393
Number of HSP's gapped (non-prelim): 91
length of query: 452
length of database: 10,339,736
effective HSP length: 103
effective length of query: 349
effective length of database: 10,337,161
effective search space: 3607669189
effective search space used: 3607669189
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)