TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2) # Homo sapiens # Complete (448 letters) Database: S.arctica/genome.fa 15,618 sequences; 121,588,341 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value supercont1.927 of Sphaeroforma arctica JP610 89 2e-17 >supercont1.927 of Sphaeroforma arctica JP610 Length = 30644 Score = 89.4 bits (220), Expect = 2e-17 Identities = 43/78 (55%), Positives = 54/78 (69%) Frame = +2 Query: 218 NPWIIIGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKVKM 277 NPW I+GGVA V + I P++A GDV+VLTKPLGTQ+AVN HQWLD P+ W++VK Sbjct: 3908 NPWPILGGVAQSVMLATDVITPENARAGDVMVLTKPLGTQLAVNLHQWLDQPKWWDQVKH 4087 Query: 278 VVSREEVELAYQEAMFNM 295 V+ E V AY A +M Sbjct: 4088 VIDAETVIRAYNVASASM 4141 Score = 67.8 bits (164), Expect = 6e-11 Identities = 29/43 (67%), Positives = 36/43 (83%) Frame = +3 Query: 130 GLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDN 172 G+ +V TTDFFYPLV+DPY G+IACANVLSD+YA+G + DN Sbjct: 2586 GVVMVSTTDFFYPLVDDPYTQGQIACANVLSDMYAVGARKVDN 2714 Score = 57.8 bits (138), Expect = 6e-08 Identities = 27/48 (56%), Positives = 35/48 (72%) Frame = +1 Query: 298 LNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLP 345 LN A LMH NA AATD+TGFGILGH+ NLA+ Q+ +++ I +LP Sbjct: 4594 LNMVGAQLMHKHNARAATDVTGFGILGHANNLAQAQKAKLTLRIDSLP 4737 Score = 43.1 bits (100), Expect = 0.002 Identities = 24/54 (44%), Positives = 31/54 (57%) Frame = +3 Query: 353 VSKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGI 406 V K F L G SAETSGGLL+C+ A FC I + EG +A++VG+ Sbjct: 6105 VLKGGALFNLATGYSAETSGGLLMCMAPADADAFCRAIHAE---EGWKAFVVGM 6257 Database: S.arctica/genome.fa Posted date: Nov 21, 2011 7:47 PM Number of letters in database: 121,588,341 Number of sequences in database: 15,618 Lambda K H 0.320 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 51,546,282 Number of Sequences: 15618 Number of extensions: 724339 Number of successful extensions: 2642 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 624 Number of HSP's successfully gapped in prelim test: 127 Number of HSP's that attempted gapping in prelim test: 1827 Number of HSP's gapped (non-prelim): 1194 length of query: 448 length of database: 40,529,447 effective HSP length: 112 effective length of query: 336 effective length of database: 38,780,231 effective search space: 13030157616 effective search space used: 13030157616 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 67 (30.4 bits)