TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Homo sapiens # Complete
         (448 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig2813  13099 14725                                                45   7e-04
Contig84498  680 680                                                   39   0.040
Contig4295  8964 17768                                                 32   6.3  

>Contig2813  13099 14725
          Length = 14725

 Score = 45.1 bits (105), Expect = 7e-04
 Identities = 20/34 (58%), Positives = 25/34 (73%)
 Frame = -2

Query: 133  LVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMG 166
            ++ T DFF P+V+D Y  G IA AN LSD+YAMG
Sbjct: 6507 II*TVDFFTPVVDDAYTFGAIAAANSLSDVYAMG 6406



 Score = 35.0 bits (79), Expect = 0.75
 Identities = 24/91 (26%), Positives = 42/91 (46%), Gaps = 1/91 (1%)
 Frame = -1

Query: 230  VCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKVKMVVSREEVELA-Y 288
            +  P++    ++A  GD ++LTK +GT +   A                + R     A  
Sbjct: 5776 IVHPHKIWKNNTARDGDAIILTKRIGTGICTTA----------------MKRNMASPATA 5645

Query: 289  QEAMFNMATLNRTAAGLMHTFNAHAATDITG 319
            Q A+  M+ LN+ AA ++  +  H+ TD+TG
Sbjct: 5644 QAAIKTMSELNKYAAEVLLKYKVHSCTDVTG 5552


>Contig84498  680 680
          Length = 680

 Score = 39.3 bits (90), Expect = 0.040
 Identities = 17/31 (54%), Positives = 22/31 (70%)
 Frame = -2

Query: 133 LVQTTDFFYPLVEDPYMMGRIACANVLSDLY 163
           ++ T DFF P+V+D Y  G IA AN LSD+Y
Sbjct: 94  II*TVDFFTPVVDDAYTFGAIAAANSLSDVY 2


>Contig4295  8964 17768
          Length = 17768

 Score = 32.0 bits (71), Expect = 6.3
 Identities = 17/32 (53%), Positives = 19/32 (59%)
 Frame = -2

Query: 86   GRGLVGGQEEASQEAGLPAGAGPSPTFPALGI 117
            GRG   G EE +   GLPAG G SPT  +L I
Sbjct: 4669 GRGEGEGGEEGALSPGLPAGGGESPTALSLEI 4574


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 139,984,951
Number of Sequences: 297657
Number of extensions: 2069225
Number of successful extensions: 7657
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 7347
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7657
length of query: 448
length of database: 106,339,350
effective HSP length: 117
effective length of query: 331
effective length of database: 71,513,481
effective search space: 23670962211
effective search space used: 23670962211
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 69 (31.2 bits)