TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Homo sapiens # Complete
         (448 letters)

Database: I.multifiliis_strain_G5/genome.fa 
           2017 sequences; 48,799,969 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplace...    82   2e-48

>gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251335, whole genome shotgun
             sequence
          Length = 70427

 Score = 81.6 bits (200), Expect(3) = 2e-48
 Identities = 48/122 (39%), Positives = 71/122 (58%)
 Frame = -1

Query: 305   LMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASGRFGLLQ 364
             LM  + A+A TD+TGFGI GHS  LA+  +  V  +I+NLP I  +A++   +  F    
Sbjct: 41075 LMKKYKANACTDVTGFGIKGHS*FLAEV*KE*VDLIINNLPTIKYVASIDGKARDFKFK* 40896

Query: 365   GTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIE 424
             G S ETSGGLLICL + +A  F SE+K +    G +  +VG V KG   + + +   + E
Sbjct: 40895 GLSPETSGGLLICLEKNKAKEFISEMKDN----GLECNLVGEVVKGKNNSIVREDASIEE 40728

Query: 425   VL 426
             ++
Sbjct: 40727 II 40722



 Score = 79.0 bits (193), Expect(3) = 2e-48
 Identities = 57/151 (37%), Positives = 73/151 (48%), Gaps = 6/151 (3%)
 Frame = -3

Query: 27    TLGRSFSNYRP-----FEPQALGLSPSWRLTGFSGMKGUGCKVPQEALLKLLAGLTRPDV 81
             T  + + N RP     + P    L  ++ LT +S +KG GCKVPQ  L K L  L     
Sbjct: 41889 TTKKPYINGRP*KRPLWNPTDHNLEKTFVLTDYSDLKG*GCKVPQADLKKYLEKL----- 41725

Query: 82    RPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALGIGMDSCVIPLRHGGLS-LVQTTDFF 140
                      G  +  + EA                   D  V P+R+     L+ T DFF
Sbjct: 41724 ---------GNAK*ITSEAP------------------DVSVRPVRNTKTKKLISTIDFF 41626

Query: 141   YPLVEDPYMMGRIACANVLSDLYAMGITECD 171
             YPLVEDPYM GRIAC NVLSDL++MG+T+ D
Sbjct: 41625 YPLVEDPYM*GRIACCNVLSDLFSMGVTDID 41533



 Score = 72.4 bits (176), Expect(3) = 2e-48
 Identities = 44/111 (39%), Positives = 63/111 (56%), Gaps = 2/111 (1%)
 Frame = -2

Query: 191   VTPLMVKGFRDXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQPNEFIMPDSAVVGDVLVL 250
             VT LM+ GF +                NPW IIGGV   V +  EF+MP++A VGD+++L
Sbjct: 41422 VTSLMI*GFDECA**VGAYVLGG*TIINPWPIIGGVGISVVEREEFLMPNNAKVGDMIIL 41243

Query: 251   TKPLGTQVAVNAHQWL--DNPERWNKVKMVVSREEVELAYQEAMFNMATLN 299
             TKPLGT +AVN   W   D  ++  K+   +S+E V+ A+ +A   M TL+
Sbjct: 41242 TKPLGT*LAVNLK*WYTKDIKDKLEKI*GFISKE*VD*AFLKAEMEMGTLS 41090


  Database: I.multifiliis_strain_G5/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 48,799,969
  Number of sequences in database:  2017
  
Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,112,639
Number of Sequences: 2017
Number of extensions: 97945
Number of successful extensions: 95
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 89
Number of HSP's gapped (non-prelim): 10
length of query: 448
length of database: 16,266,656
effective HSP length: 106
effective length of query: 342
effective length of database: 16,052,854
effective search space: 5490076068
effective search space used: 5490076068
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 64 (29.3 bits)