TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Homo sapiens # Complete
         (448 letters)

Database: F.cylindrus/genome.fa 
           271 sequences; 80,540,407 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_21                                                           221   3e-57

>scaffold_21
          Length = 951085

 Score =  221 bits (562), Expect = 3e-57
 Identities = 131/335 (39%), Positives = 172/335 (51%), Gaps = 31/335 (9%)
 Frame = -2

Query: 122    CVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNXXXXXXXXX 181
              C I     G  ++ TTDFF+PLV+ PY+ GRI  ANVLSDLYA G+  CD          
Sbjct: 153033 CSIRETRQGHYVISTTDFFFPLVDSPYLQGRIGAANVLSDLYAEGVQHCDFVLMLLAASL 152854

Query: 182    XXXXXXREKVTPLMVKGFRDXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQPNEFIMPDS 241
                    R   T LMV+GFRD                NPW IIGGVAT V   +E++  D 
Sbjct: 152853 DMTPQDRTICTNLMVRGFRDACTEAETTITGGQTVLNPWAIIGGVATSVVTRDEYVSSDG 152674

Query: 242    AVVGDVLVLTKPLGTQVAVNAHQWLDNPER------------WNKV-------KMVVSRE 282
              A VGDV+VLTKP+GTQ+AVN H+W    E+            WNK+          ++  
Sbjct: 152673 ACVGDVIVLTKPIGTQIAVNVHEWRKRHEKKAKNGDTTGDKNWNKILEQNKNGDEFLTVS 152494

Query: 283    EVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIH 342
              E E+    A+ +M+ LNR  + LM   NAHA TD+TGFG+LGH+QNL   Q  +V   +H
Sbjct: 152493 ESEIMMHAAVRSMSKLNRFGSKLMTKHNAHAGTDVTGFGLLGHAQNLMDNQIAKVGIELH 152314

Query: 343    NLPIIAKMAAVSKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIK----------- 391
                P IA    ++     F L  G SAETSGGL+I +    A  +C+E++           
Sbjct: 152313 TFPCIANTLKINDNIMNFRLRDGYSAETSGGLMISMTEADAIAYCAELREVETVAYNYNT 152134

Query: 392    SSKYGEGHQAWIVG-IVEKGNRTARIIDKPRVIEV 425
              SS  G     WI+G +VE  +R A I +    +EV
Sbjct: 152133 SSDAGTDFGPWIIGRVVESADRKATITEDAVYLEV 152029



 Score = 43.1 bits (100), Expect = 0.001
 Identities = 18/39 (46%), Positives = 25/39 (64%)
 Frame = -1

Query: 38     FEPQALGLSPSWRLTGFSGMKGUGCKVPQEALLKLLAGL 76
              F+P ++GL   W LT FS +KG  CK+PQ  L  +L+ L
Sbjct: 153418 FDPTSVGLPEGWSLTDFSDLKG*ACKIPQNELQDMLSSL 153302


  Database: F.cylindrus/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 80,540,407
  Number of sequences in database:  271
  
Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,864,107
Number of Sequences: 271
Number of extensions: 395175
Number of successful extensions: 1215
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1192
Number of HSP's gapped (non-prelim): 61
length of query: 448
length of database: 26,846,802
effective HSP length: 110
effective length of query: 338
effective length of database: 26,816,992
effective search space: 9064143296
effective search space used: 9064143296
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)