TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Homo sapiens # Complete
         (448 letters)

Database: D.discoideum_AX4/genome.fa 
           6 sequences; 33,943,072 total letters

Searching......done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|93569092|gb|CM000152.2| Dictyostelium discoideum AX4 chromoso...   273   2e-73

>gi|93569092|gb|CM000152.2| Dictyostelium discoideum AX4 chromosome 3, whole
               genome shotgun sequence
          Length = 6357299

 Score =  273 bits (699), Expect = 2e-73
 Identities = 147/297 (49%), Positives = 181/297 (60%), Gaps = 8/297 (2%)
 Frame = +3

Query: 139     FFYPLVEDPYMMGRIACANVLSDLYAMGITECDNXXXXXXXXXXXXXXXREKVTPLMVKG 198
               FF+PLV+DPY  G+IACANVLSDLY+ GI +CDN               R+  + LM++G
Sbjct: 5741658 FFFPLVDDPYFQGKIACANVLSDLYSFGIEDCDNMLMLLACSTDMTAEQRQWSSKLMIQG 5741837

Query: 199     FRDXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQV 258
               F D                NPW I+GGVAT + + NEFI P +AV GDVLVLTKPLGTQV
Sbjct: 5741838 FNDQAICAGSKVSGGQTVKNPWPIVGGVATSILKTNEFIKPVNAVPGDVLVLTKPLGTQV 5742017

Query: 259     AVNAHQWLDNPERWNKVKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDIT 318
                VN HQWL  PERW K+  + + EE E  +  A  +MA LNR  A LM  +NAHAATD+T
Sbjct: 5742018 CVNFHQWLSKPERWEKINTITNAEECEQVFNYATLSMARLNRVGARLMKKYNAHAATDVT 5742197

Query: 319     GFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAV-SKASGRFGLLQGTSAETSGGLLIC 377
               GFGILGHS NLA+ Q   + F IH LPII  M  +    +  F LL+GTSAETSGGLLI 
Sbjct: 5742198 GFGILGHSTNLAQNQLLPIRFEIHTLPIIKHMKKLEDHLNHPFKLLKGTSAETSGGLLIS 5742377

Query: 378     LPREQAARFCSEIKSSKYGEGHQAWIVGIV-------EKGNRTARIIDKPRVIEVLP 427
               + RE A  FC EI      E   AWI+G V       ++   T+ I++ P++IEV P
Sbjct: 5742378 MSRENAEAFCKEIYEI---EKQPAWIIGDVIDQSSNYDRSKNTSIILENPKIIEVEP 5742539



 Score = 36.6 bits (83), Expect = 0.042
 Identities = 17/27 (62%), Positives = 20/27 (74%)
 Frame = +1

Query: 50      RLTGFSGMKGUGCKVPQEALLKLLAGL 76
               RLT F+ +KG GCKVPQ  LL LL G+
Sbjct: 5741398 RLTDFTKLKG*GCKVPQAELLSLLDGI 5741478


  Database: D.discoideum_AX4/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 33,943,072
  Number of sequences in database:  6
  
Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,759,350
Number of Sequences: 6
Number of extensions: 118742
Number of successful extensions: 259
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 244
Number of HSP's gapped (non-prelim): 15
length of query: 448
length of database: 11,314,357
effective HSP length: 104
effective length of query: 344
effective length of database: 11,313,733
effective search space: 3891924152
effective search space used: 3891924152
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)