TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Drosophila melanogaster # Complete
         (370 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig2813  13099 14725                                                42   0.004
Contig84498  680 680                                                   38   0.068
Contig189120  1515 1515                                                31   8.4  

>Contig2813  13099 14725
          Length = 14725

 Score = 42.4 bits (98), Expect = 0.004
 Identities = 21/34 (61%), Positives = 25/34 (73%)
 Frame = -2

Query: 71   LIQTVDFFYPMVNDPELLGRIALANVLSDVYAVG 104
            +I TVDFF P+V+D    G IA AN LSDVYA+G
Sbjct: 6507 II*TVDFFTPVVDDAYTFGAIAAANSLSDVYAMG 6406



 Score = 40.0 bits (92), Expect = 0.018
 Identities = 25/81 (30%), Positives = 43/81 (53%)
 Frame = -1

Query: 181  SNAQPGDVLVLTKPLGGQMAMDAHLWQLNQTEKYKKLLSECSDADIKETFEIAVKSMTYL 240
            + A+ GD ++LTK +G  +           T   K+ ++  + A      + A+K+M+ L
Sbjct: 5746 NTARDGDAIILTKRIGTGIC----------TTAMKRNMASPATA------QAAIKTMSEL 5615

Query: 241  NKNAALLMHKYQAHCATDITG 261
            NK AA ++ KY+ H  TD+TG
Sbjct: 5614 NKYAAEVLLKYKVHSCTDVTG 5552



 Score = 26.9 bits (58), Expect(2) = 4.2
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = -2

Query: 229  TFEIAVKSMTYLNKNAALLMHKYQAHCATDIT 260
            T +  +K+M+ LNK AA ++ KY+     D+T
Sbjct: 5484 TAQEVIKTMSELNKYAAEVLLKYKVSSGADVT 5389



 Score = 23.5 bits (49), Expect(2) = 4.2
 Identities = 10/30 (33%), Positives = 17/30 (56%)
 Frame = -3

Query: 261  GFGLLGHANNLAQFQKEKVLFQINKLPIIK 290
            GFGL GH   +A+     V    +++P+I+
Sbjct: 5324 GFGLAGHMWEMAKSSDLSVHLYFDQIPLIE 5235


>Contig84498  680 680
          Length = 680

 Score = 38.1 bits (87), Expect = 0.068
 Identities = 19/31 (61%), Positives = 22/31 (70%)
 Frame = -2

Query: 71  LIQTVDFFYPMVNDPELLGRIALANVLSDVY 101
           +I TVDFF P+V+D    G IA AN LSDVY
Sbjct: 94  II*TVDFFTPVVDDAYTFGAIAAANSLSDVY 2


>Contig189120  1515 1515
          Length = 1515

 Score = 31.2 bits (69), Expect = 8.4
 Identities = 17/50 (34%), Positives = 24/50 (48%)
 Frame = -3

Query: 161 WCIIGGIATSVCRSEEIILPSNAQPGDVLVLTKPLGGQMAMDAHLWQLNQ 210
           WC++ GI    C     +LPS      +L L+ P        +HL+QLNQ
Sbjct: 373 WCLLEGIVAKSCPLTFFLLPSYLSLSLLLYLSSPY-----PSSHLFQLNQ 239


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 121,307,616
Number of Sequences: 297657
Number of extensions: 1623988
Number of successful extensions: 5262
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 5088
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5262
length of query: 370
length of database: 106,339,350
effective HSP length: 115
effective length of query: 255
effective length of database: 72,108,795
effective search space: 18387742725
effective search space used: 18387742725
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 68 (30.8 bits)