TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Drosophila melanogaster # Complete
         (370 letters)

Database: I.multifiliis_strain_G5/genome.fa 
           2017 sequences; 48,799,969 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplace...    98   1e-47

>gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251335, whole genome shotgun
             sequence
          Length = 70427

 Score = 98.2 bits (243), Expect(3) = 1e-47
 Identities = 52/135 (38%), Positives = 78/135 (57%)
 Frame = -3

Query: 1     MFQPEKHGLEPDFQLTKFTTHTGUSCKIPQKVLEKYLRGTEIENKNNDGYLIGSGMDCAV 60
             ++ P  H LE  F LT ++   G  CK+PQ  L+KYL     E   N   +     D +V
Sbjct: 41844 LWNPTDHNLEKTFVLTDYSDLKG*GCKVPQADLKKYL-----EKLGNAK*ITSEAPDVSV 41680

Query: 61    IPLKRHKDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIVSTSTS 120
              P++  K   LI T+DFFYP+V DP + GRIA  NVLSD++++GVT  DTV M+++ S  
Sbjct: 41679 RPVRNTKTKKLISTIDFFYPLVEDPYM*GRIACCNVLSDLFSMGVTDIDTVLMVLAVSNK 41500

Query: 121   FSEKERDVVIGLVMK 135
              +  +++V+   + K
Sbjct: 41499 MNA*QKEVIYIYIYK 41455



 Score = 73.6 bits (179), Expect(3) = 1e-47
 Identities = 50/130 (38%), Positives = 73/130 (56%)
 Frame = -1

Query: 239   YLNKNAALLMHKYQAHCATDITGFGLLGHANNLAQFQKEKVLFQINKLPIIKNVLKFSTL 298
             Y    A  LM KY+A+  TD+TGFG+ GH+  LA+  KE V   IN LP IK V      
Sbjct: 41099 YFEYFAKFLMKKYKANACTDVTGFGIKGHS*FLAEV*KE*VDLIINNLPTIKYVASIDG- 40923

Query: 299   VGQSTKFRSGRSVETSGGLLICLPADAADKFCRDFEEATNGEQKSFQIGHVTAANESDAV 358
               +  KF+ G S ETSGGLLICL  + A +F  + ++  NG + +  +G V    +++++
Sbjct: 40922 KARDFKFK*GLSPETSGGLLICLEKNKAKEFISEMKD--NGLECNL-VGEV-VKGKNNSI 40755

Query: 359   LCEDVEFIEV 368
             + ED    E+
Sbjct: 40754 VREDASIEEI 40725



 Score = 58.5 bits (140), Expect(3) = 1e-47
 Identities = 25/49 (51%), Positives = 36/49 (73%)
 Frame = -2

Query: 158   INPWCIIGGIATSVCRSEEIILPSNAQPGDVLVLTKPLGGQMAMDAHLW 206
             INPW IIGG+  SV   EE ++P+NA+ GD+++LTKPLG  +A++   W
Sbjct: 41344 INPWPIIGGVGISVVEREEFLMPNNAKVGDMIILTKPLGT*LAVNLK*W 41198


  Database: I.multifiliis_strain_G5/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 48,799,969
  Number of sequences in database:  2017
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,485,187
Number of Sequences: 2017
Number of extensions: 260993
Number of successful extensions: 739
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 62
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 657
Number of HSP's gapped (non-prelim): 126
length of query: 370
length of database: 16,266,656
effective HSP length: 105
effective length of query: 265
effective length of database: 16,054,871
effective search space: 4254540815
effective search space used: 4254540815
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)