TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Drosophila melanogaster # Complete
         (370 letters)

Database: D.discoideum_AX4/genome.fa 
           6 sequences; 33,943,072 total letters

Searching......done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|93569092|gb|CM000152.2| Dictyostelium discoideum AX4 chromoso...   211   4e-58
gi|93569069|gb|CM000154.2| Dictyostelium discoideum AX4 chromoso...    29   5.2  
gi|256541943|gb|CM000151.3| Dictyostelium discoideum AX4 chromos...    28   8.9  

>gi|93569092|gb|CM000152.2| Dictyostelium discoideum AX4 chromosome 3, whole
               genome shotgun sequence
          Length = 6357299

 Score =  211 bits (537), Expect(2) = 4e-58
 Identities = 118/306 (38%), Positives = 186/306 (60%), Gaps = 6/306 (1%)
 Frame = +3

Query: 69      YLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIVSTSTSFSEKERDV 128
               +++     FF+P+V+DP   G+IA ANVLSD+Y+ G+   D + M+++ ST  + ++R  
Sbjct: 5741634 FIIFLKKKFFFPLVDDPYFQGKIACANVLSDLYSFGIEDCDNMLMLLACSTDMTAEQRQW 5741813

Query: 129     VIGLVMKGFQNSLKANGYRNTPLIIRQLKINPWCIIGGIATSVCRSEEIILPSNAQPGDV 188
                  L+++GF +     G + +     Q   NPW I+GG+ATS+ ++ E I P NA PGDV
Sbjct: 5741814 SSKLMIQGFNDQAICAGSKVSG---GQTVKNPWPIVGGVATSILKTNEFIKPVNAVPGDV 5741984

Query: 189     LVLTKPLGGQMAMDAHLWQLNQTEKYKKLLSECSDADIKETFEIAVKSMTYLNKNAALLM 248
               LVLTKPLG Q+ ++ H W L++ E+++K+ +  +  + ++ F  A  SM  LN+  A LM
Sbjct: 5741985 LVLTKPLGTQVCVNFHQW-LSKPERWEKINTITNAEECEQVFNYATLSMARLNRVGARLM 5742161

Query: 249     HKYQAHCATDITGFGLLGHANNLAQFQKEKVLFQINKLPIIKNVLKFSTLVGQSTKFRSG 308
                KY AH ATD+TGFG+LGH+ NLAQ Q   + F+I+ LPIIK++ K    +    K   G
Sbjct: 5742162 KKYNAHAATDVTGFGILGHSTNLAQNQLLPIRFEIHTLPIIKHMKKLEDHLNHPFKLLKG 5742341

Query: 309     RSVETSGGLLICLPADAADKFCRDFEEATNGEQKSFQIGHVT------AANESDAVLCED 362
                S ETSGGLLI +  + A+ FC++  E    +Q ++ IG V         +++ +++ E+
Sbjct: 5742342 TSAETSGGLLISMSRENAEAFCKEIYEIE--KQPAWIIGDVIDQSSNYDRSKNTSIILEN 5742515

Query: 363     VEFIEV 368
                + IEV
Sbjct: 5742516 PKIIEV 5742533



 Score = 32.7 bits (73), Expect(2) = 4e-58
 Identities = 23/63 (36%), Positives = 29/63 (46%)
 Frame = +1

Query: 14      QLTKFTTHTGUSCKIPQKVLEKYLRGTEIENKNNDGYLIGSGMDCAVIPLKRHKDYLLIQ 73
               +LT FT   G  CK+PQ  L   L          DG   G G DC++   K   D ++IQ
Sbjct: 5741398 RLTDFTKLKG*GCKVPQAELLSLL----------DGIGEGIGYDCSISQTK-FPDIVMIQ 5741544

Query: 74      TVD 76
               T D
Sbjct: 5741545 TTD 5741553



 Score = 30.8 bits (68), Expect = 1.8
 Identities = 14/54 (25%), Positives = 27/54 (50%)
 Frame = -3

Query: 26      CKIPQKVLEKYLRGTEIENKNNDGYLIGSGMDCAVIPLKRHKDYLLIQTVDFFY 79
               CK   K  +K  +   ++ KNN+ Y+    + C  +P K+   YL   ++ +F+
Sbjct: 1621365 CKTEYKNQKKNCKNKTLKKKNNNKYIFIKTLWCFHLPYKKKYKYLTFLSLYYFF 1621204


>gi|93569069|gb|CM000154.2| Dictyostelium discoideum AX4 chromosome 5, whole
               genome shotgun sequence
          Length = 5125352

 Score = 29.3 bits (64), Expect = 5.2
 Identities = 22/89 (24%), Positives = 40/89 (44%)
 Frame = -3

Query: 206     WQLNQTEKYKKLLSECSDADIKETFEIAVKSMTYLNKNAALLMHKYQAHCATDITGFGLL 265
               W +N  E     L   +D D++   E  + +    N+    L+ KYQ   +  +      
Sbjct: 4166145 WGIN-IESISYKLDNLNDPDLQNYDENQIYNWLE-NEEIYQLIKKYQ-QSSIIVNNNNNF 4165975

Query: 266     GHANNLAQFQKEKVLFQINKLPIIKNVLK 294
                + N L +++ + +LF   K+  IKN+LK
Sbjct: 4165974 DNINKLKEYKNQSILFNNQKIDDIKNILK 4165888



 Score = 29.3 bits (64), Expect = 5.2
 Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 3/69 (4%)
 Frame = +1

Query: 246     LLMHKYQAHCATDITGFGLLGHANNLAQFQ---KEKVLFQINKLPIIKNVLKFSTLVGQS 302
               LL+ K+  +   + T   ++ + NN +  +   K K +F+     I KN+L FST     
Sbjct: 2818282 LLIIKFNVYIIIN*THKYIIMNLNNFSLIRDLAKNKHIFKFKTDNINKNILPFSTKYEPK 2818461

Query: 303     TKFRSGRSV 311
               +KF++  S+
Sbjct: 2818462 SKFKNNESI 2818488


>gi|256541943|gb|CM000151.3| Dictyostelium discoideum AX4 chromosome 2,
               whole genome shotgun sequence
          Length = 8484197

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 13/34 (38%), Positives = 21/34 (61%)
 Frame = -2

Query: 214     YKKLLSECSDADIKETFEIAVKSMTYLNKNAALL 247
               Y++ + E  + ++KE  EI  KS+  LNK  +LL
Sbjct: 4143472 YRQEIKEYKEREVKEIKEIKEKSLAELNKKKSLL 4143371


  Database: D.discoideum_AX4/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 33,943,072
  Number of sequences in database:  6
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,155,305
Number of Sequences: 6
Number of extensions: 195002
Number of successful extensions: 700
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 570
Number of HSP's gapped (non-prelim): 367
length of query: 370
length of database: 11,314,357
effective HSP length: 102
effective length of query: 268
effective length of database: 11,313,745
effective search space: 3032083660
effective search space used: 3032083660
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)