TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000056_1.0 # Protein # Selenophosphate synthetase 2 (SPS2) # Anopheles gambiae # Complete (369 letters) Database: S.arctica/genome.fa 15,618 sequences; 121,588,341 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value supercont1.927 of Sphaeroforma arctica JP610 72 3e-12 supercont1.404 of Sphaeroforma arctica JP610 30 7.8 >supercont1.927 of Sphaeroforma arctica JP610 Length = 30644 Score = 71.6 bits (174), Expect = 3e-12 Identities = 35/87 (40%), Positives = 50/87 (57%) Frame = +2 Query: 156 GSIAENPWCVIGGAASAVCHRSELIMPYNAQPGDALVLTKPLGTQLATNAYIWMGEQQSD 215 G NPW ++GG A +V +++I P NA+ GD +VLTKPLGTQLA N + W+ Q Sbjct: 3893 GQSVMNPWPILGGVAQSVMLATDVITPENARAGDVMVLTKPLGTQLAVNLHQWL--DQPK 4066 Query: 216 SWAQLRERFTVADIEQTYRIALESMSR 242 W Q++ + + Y +A SM R Sbjct: 4067 WWDQVKHVIDAETVIRAYNVASASMKR 4147 Score = 62.4 bits (150), Expect = 2e-09 Identities = 30/56 (53%), Positives = 38/56 (67%) Frame = +1 Query: 235 IALESMSRLNKTGAELMKKYGAHAATDVTGFGLYGHAENLASHQTADVDFHLDTLP 290 I + + RLN GA+LM K+ A AATDVTGFG+ GHA NLA Q A + +D+LP Sbjct: 4570 IYCQYLLRLNMVGAQLMHKHNARAATDVTGFGILGHANNLAQAQKAKLTLRIDSLP 4737 Score = 59.3 bits (142), Expect = 2e-08 Identities = 24/47 (51%), Positives = 38/47 (80%) Frame = +3 Query: 76 LVQSVDFFYPLIDDPFMLGKIALANVVSDVFAVGATEIDQIKLIVTA 122 +V + DFFYPL+DDP+ G+IA ANV+SD++AVGA ++D + +I+ + Sbjct: 2595 MVSTTDFFYPLVDDPYTQGQIACANVLSDMYAVGARKVDNMLMILAS 2735 Score = 37.0 bits (84), Expect = 0.083 Identities = 24/72 (33%), Positives = 33/72 (45%), Gaps = 2/72 (2%) Frame = +3 Query: 279 TADVDFHLDTLPIIKNVREIAETLGRGA--KLLAGKAVETSGGLLICLPREAAAGFCEEY 336 T VD + +IK + L GA L G + ETSGGLL+C+ A FC Sbjct: 6039 TTSVDSVYYPISVIKGTIAVDRVLKGGALFNLATGYSAETSGGLLMCMAPADADAFCRAI 6218 Query: 337 RRCTKHEAWIVG 348 +A++VG Sbjct: 6219 HAEEGWKAFVVG 6254 Score = 30.4 bits (67), Expect = 7.8 Identities = 13/20 (65%), Positives = 15/20 (75%) Frame = +3 Query: 4 PESYGLSHDFRLTKFSTLRG 23 P YGL DFRLT +STL+G Sbjct: 1524 PTVYGLPTDFRLTDYSTLKG 1583 >supercont1.404 of Sphaeroforma arctica JP610 Length = 77350 Score = 30.4 bits (67), Expect = 7.8 Identities = 12/38 (31%), Positives = 22/38 (57%) Frame = -3 Query: 267 LYGHAENLASHQTADVDFHLDTLPIIKNVREIAETLGR 304 +YG + +H V++HL PI+ +++E E +GR Sbjct: 16112 VYGCVQRCRAHDIQTVEWHLAVEPIMVHIKESLEAIGR 15999 Database: S.arctica/genome.fa Posted date: Nov 21, 2011 7:47 PM Number of letters in database: 121,588,341 Number of sequences in database: 15,618 Lambda K H 0.319 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 46,833,158 Number of Sequences: 15618 Number of extensions: 642920 Number of successful extensions: 2148 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 573 Number of HSP's successfully gapped in prelim test: 93 Number of HSP's that attempted gapping in prelim test: 1428 Number of HSP's gapped (non-prelim): 1021 length of query: 369 length of database: 40,529,447 effective HSP length: 111 effective length of query: 258 effective length of database: 38,795,849 effective search space: 10009329042 effective search space used: 10009329042 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 66 (30.0 bits)